狂犬病病毒SRV-9株对小鼠脑神经原代细胞的感染及毒力观察

为建立一种狂犬病病毒(RV)感染原代神经细胞的体外培养方法,分离培养了新生小鼠脑神经原代细胞,并进行了RV感染试验,利用免疫荧光技术和免疫细胞化学试验检测了神经细胞中的RV及其对被感染细胞的影响.结果显示,RV可感染分离的原代神经细胞,并可以检测到针对RV的特异性荧光.表明,建立了可培养RV的原代神经细胞的制备方法,并...     

Youth organisations as learning organisations: exploring special contributions and challenges

Youth and youth organisations are becoming valuable development partners, but little knowledge about their characteristics as learning organisations exists. This article presents perceptions of youth workers on this topic. These were gathered via an online survey and through research done by a youth network. Knowledge, skills and attitudes for active citizenship are facilitated by youth organisations as emergent learning spaces where peer-to-peer learning and experiential methods are central. Youth organisations adapt existing toolboxes and develop their own tools and knowledge that are more relevant to their needs. Support for youth organisations should take into account these special ways of learning.

Organisations de jeunes en tant qu'organisations qui apprennent : étude des contributions spéciales et des défis

Les jeunes et les organisations de jeunes sont en passe de devenir des partenaires de développement très utiles, mais il n'existe guère de connaissances sur leurs caractéristiques en tant qu'organisations qui apprennent. Cet article présente les manières dont les personnes qui travaillent avec les jeunes perçoivent ce sujet. Ces perceptions ont été recueillies au moyen d'un questionnaire en ligne et de recherches menées par un réseau de jeunes. Les connaissances, compétences et attitudes pour la citoyenneté active sont facilitées par des organisations de jeunes en tant que nouveaux espaces propices à l'apprentissage, dont les piliers sont l'apprentissage entre pairs et les méthodes expérientielles. Les organisations de jeunes adaptent les boîtes à outils existantes et mettent au point leurs propres outils et connaissances qui pourvoient mieux à leurs besoins. Le soutien apporté aux organisations de jeunes devrait tenir compte de ces façons spéciales d'apprendre.

Organizações de jovens enquanto organizações de aprendizado: Explorando contribuições especiais e desafios

Os jovens e as organizações de jovens estão se tornando valiosos parceiros de desenvolvimento, mas existe pouco conhecimento sobre suas características enquanto organizações de aprendizado. Este artigo apresenta percepções de jovens trabalhadores sobre este tópico. Elas foram reunidas através de um levantamento online e através de pesquisa realizada por uma rede de jovens. Conhecimento, habilidades e atitudes para uma cidadania ativa são promovidos por organizações de jovens como espaços de aprendizado emergentes, onde o aprendizado entre pares e métodos experimentais são centrais. As organizações de jovens adaptam caixas de ferramentas existentes e desenvolvem suas próprias ferramentas e conhecimento que sejam mais relevantes para suas necessidades. O suporte a organizações de jovens deve levar em conta estas formas especiais de aprendizado.

Organizaciones juveniles como organizaciones de aprendizaje: un examen de sus aportes y retos

La juventud y las organizaciones de jóvenes se han convertido en sectores importantes del desarrollo pero se conocen poco sus cualidades como organizaciones de aprendizaje. Este ensayo analiza qué opinan los trabajadores jóvenes sobre este tema basándose en encuestas en línea e investigaciones realizadas por una red de jóvenes. Las organizaciones juveniles cuentan con los conocimientos, herramientas y actitudes para que pueda ejercerse una ciudadanía activa, siendo a la vez espacios emergentes de aprendizaje que se centran en el aprendizaje entre pares y donde los métodos vivenciales son fundamentales. Las organizaciones juveniles adaptan las metodologías que ya existen y generan metodologías y conocimientos propios, más adaptados a sus necesidades. Las instancias que apoyen a las organizaciones juveniles deberán tomar en cuenta estas formas especiales de aprendizaje.     

中国韩国学研究的现状、特征与趋势——基于1998～2010年CSSCI数据

当前,韩国学研究已成为国内重要国别研究领域。本文运用文献计量学的方法,对1998～2010年间CSSCI数据库收录的韩国学论文数量与被引频次数量、合作论文比重与性质、作者机构分布、发文前10个单位分布、第一作者所属地区分布、发文期刊数量及载文量前10个期刊分布等进行统计,从各学科论文、作者及期刊三个方面解析国内韩国学研究现状与特征,把握其发展趋势,为国内韩国学研究的发展提供借鉴,推动国内韩国学研究更好地服务于我国经济社会发展。     

The influence of functional and geographical diversity in collaboration on product innovation performance in SMEs

Collaboration in innovation is considered an effective strategy to overcome barriers to innovate in SMEs. However, resource constraints and risk of knowledge leakage force management in SMEs to be selective with whom and where to collaborate. Still little is known about interactions between types of partners and their geographical location. This paper sheds new light on the relation between functional and geographical diversity in innovation partners and new-to-the-market and new-to-the-firm innovation performance in SMEs. Using data from two waves (2008 and 2010) of the Community Innovation Survey for Belgium, a positive relation is found between market partners and innovation new-to-the-firm, and between science and global partners and innovation new-to-the-market. A larger diversity in type and in geographical spread of partners is found to be positively associated with innovation new-to-the-market. Cooperation with a diverse set of market partners enhances new-to-the-firm innovation. More diversity in science partners enhances the balance between new-to-the-firm and new-to-the-market innovation. Diversity among international partners enhances new-to-the-firm and new-to-the-market innovation, but not the balance between both. A cooperation strategy balancing functional with geographical partner diversity enhances the balance between new-to-the-firm versus new-to-the-market innovation.     

汗潜指印的STR分型检测

目的探索汗潜指印的荧光STR复合扩增检测的方法。方法采用Chelex-100和Microco-100浓缩柱,提取汗潜指印中DNA,STR复合扩增荧光电泳检测。结果 105例汗潜指纹STR分型可明确判读5个以上基因座的占30.3％,个体之间的差异、捺印指印时用力大小以及指印遗留在客体上时间的长短均影响检测成功率。结论该汗潜指印的DNA提取方法步骤简单,方法较为稳定,使单枚汗潜指印可望获得DNA分型。     

鸡毒霉形体弱毒疫苗的研究——鸡毒霉形体弱毒F株免疫效力测定

以点眼的方法,用鸡毒霉形体F株的培养物对1、3和20日龄鸡进行了免疫接种试验,经过致病性株R株的攻击,根据气囊损伤保护率计算,免疫接种鸡可获得85％以上的保护,在攻击后14天内,免疫接种鸡比对照鸡体重增加明显。免疫力持续至少7个半月以上。F株的冻干培养物仍保持着良好的免疫作用。试验结果表明:鸡毒霉形体F株是个优良的疫苗株,可用于生产鸡毒霉形体活疫苗。     

A new staining method for constriction marks in skin

A modified Poley's acid fuchsin-methyl green stain was used to demonstrate ligature marks in tissues taken at autopsy. This stain was compared in 30 cases with haematoxylin-eosin, Mallory's trichrome and Ogata's picro-indigocarmine stains. Using the modified Poley method, it was possible to demonstrate the compression mark in corpses even when post-mortem changes were advanced. The method was found useful in evaluating the cases when the force used was minimal or when the mark was atypical using the usual haematoxylin-eosin method.