Raman spectroscopy and microspectrophotometry of reactive dyes on cotton fibres: Analysis and detection limits

A collaborative study on Raman spectroscopy and microspectrophotometry (MSP) was carried out by members of the ENFSI (European Network of Forensic Science Institutes) European Fibres Group (EFG) on different dyed cotton fabrics. The detection limits of the two methods were tested on two cotton sets with a dye concentration ranging from 0.5 to 0.005% (w/w). This survey shows that it is possible to detect the presence of dye in fibres with concentrations below that detectable by the traditional methods of light microscopy and microspectrophotometry (MSP). The MSP detection limit for the dyes used in this study was found to be a concentration of 0.5% (w/w). At this concentration, the fibres appear colourless with light microscopy. Raman spectroscopy clearly shows a higher potential to detect concentrations of dyes as low as 0.05% for the yellow dye RY145 and 0.005% for the blue dye RB221. This detection limit was found to depend both on the chemical composition of the dye itself and on the analytical conditions, particularly the laser wavelength. Furthermore, analysis of binary mixtures of dyes showed that while the minor dye was detected at 1.5% (w/w) (30% of the total dye concentration) using microspectrophotometry, it was detected at a level as low as 0.05% (w/w) (10% of the total dye concentration) using Raman spectroscopy. This work also highlights the importance of a flexible Raman instrument equipped with several lasers at different wavelengths for the analysis of dyed fibres. The operator and the set up of the analytical conditions are also of prime importance in order to obtain high quality spectra. Changing the laser wavelength is important to detect different dyes in a mixture.     

Clinical and forensic examinations of glycemic marker 1,5-anhydroglucitol by means of high performance liquid chromatography tandem mass spectrometry

Postmortem diagnosis of diabetes and a diabetic coma can be difficult because of the lack of characteristic morphological findings. 1,5-Anhydroglucitol (1,5-AG), the 1-deoxy form of glucose, competes with glucose for reabsorption in the kidneys. Therefore, diabetics with a permanent hyperglycemia show significantly lower serum concentrations of 1,5-AG than non-diabetics. A liquid chromatography-mass spectrometric method for the determination of 1,5-AG in serum and postmortem blood was developed and validated according to international guidelines. Linearity was given between 1μg/ml and 50μg/ml. Recovery rates ranged between 70.8% and 89.8%, the limit of quantification of the procedure was 0.20μg/ml, limit of quantification was 0.55μg/ml. Serum of 199 diabetics and 116 non-diabetics and femoral blood of 31 diabetic and 27 non-diabetic deceased was measured. Average concentrations were significantly (p<0.001) higher in non-diabetics compared to diabetics ante and postmortem. Seven of the diabetics may have died because of a hyperglycemic coma indicated by a sum formula of Traub>450mg/dl. 1,5-AG average concentrations in these deceased were not significantly different to diabetics which did not die because of a diabetic coma. Concentrations of 1,5-AG give a hint for not well controlled diabetes antemortem and postmortem and can be assumed as an additional and alternative information postmortem to the measurement of HbA1c or fructosamine.     

Help-seeking in a national sample of victimized Latino women: the influence of victimization types

The current study aimed to examine formal and informal help-seeking responses to interpersonal victimization among a national sample of Latino women. In addition, an examination of help-seeking by victimization type was undertaken. Data came from the Sexual Assault Among Latinas (SALAS) study that obtained help-seeking rates among a victimized subsample of Latino women (n = 714; 35.7% of a national sample). Results show a majority (76.6%) of the victimized participants engaged in some form of help-seeking with informal resources (68.9%) more often used than formal (32.5%). Medical attention was the type of formal help-seeking sought most often among victimized women who were injured (34.7%), and parents were the most common source of informal help-seeking (26.6%). However, logistic regression analyses show that help-seeking responses were significantly affected by type of victimization. Latino women who experienced childhood victimization were significantly less likely to engage in formal and informal help-seeking. Latino women who experienced stalking were significantly less likely to engage in formal help-seeking. Victimization with a weapon was significantly related to increased odds of formal help-seeking. Thus, women respond to violence in a way that is shaped by the dynamics of the victimization experience. Practice implications include the need to increase knowledge and availability of formal help-seeking venues.     

Reproducibility of facial soft tissue thicknesses for craniofacial reconstruction using cone-beam CT images

The purpose of this study was to evaluate the reproducibility of the soft tissue (ST) thicknesses at 31 landmarks using the cone-beam computed tomography (CBCT) images obtained from 20 adult subjects. Four observers carried out ST thickness measurements using Skull Measure software, and the inter- and intra-observer error rates were evaluated. Only five of 31 landmarks showed significant differences in recorded ST thickness between the observers. When excluding inexperienced observers, only one landmark showed a significant difference between the observers. Regarding the intra-observer reproducibility, the ST thickness measurements at three landmarks showed low correlation coefficients. The results of this study indicate that CBCT images can be used to measure ST thickness with high reproducibility. However, some landmarks need to be redefined to reliably measure ST thickness on CBCT images.     

Comparison of two methods for isolating DNA from human skeletal remains for STR analysis

Abstract: The quality and efficiency of a standard organic DNA isolation method and a silica‐based method using the QIAGEN Blood Maxi Kit were compared to obtain human DNA and short tandem repeats (STRs) profiles from 39 exhumed bone samples for paternity testing. DNA samples were quantified by real‐time PCR, and STR profiles were obtained using the AmpFlSTR^® Identifiler^® PCR amplification kit. Overall, the silica‐based method recovered less DNA ranging from 0 to 147.7 ng/g (average 7.57 ng/g, median = 1.3 ng/g) than did the organic method ranging from 0 to 605 ng/g (average 44.27 ng/g, median = 5.8 ng/g). Complete profiles (16/16 loci tested) were obtained from 37/39 samples (95%) using the organic method and from 9/39 samples (23%) with the silica‐based method. Compared with a standard organic DNA isolation method, our results indicate that the published silica‐based method does not improve neither the quality nor the quantity of DNA for STR profiling.     

Situating forensic traces in time

Situating events and traces in time is an essential problem in investigations. To date, among the typical questions issued in forensic science, time has generally been unexplored. The reason for this can be traced to the complexity of the overall problem, addressed by several scientists in very limited projects usually stimulated by a specific case. Considering that such issues are recurrent and transcending the treatment of each trace separately, the formalisation of a framework to address dating issues in criminal investigation is undeniably needed. Through an iterative process consisting of extracting recurrent aspects discovered from the study of problems encountered by practitioners and reported in the literature, common mechanisms were extracted and provide understanding of underlying factors encountered in forensic practise. Three complementary approaches are thus highlighted and described to formalise a preliminary framework that can be applied for the dating of traces, objects, persons and indirectly events.     

Fatal tolperisone poisoning: autopsy and toxicology findings in three suicide cases

Tolperisone (Mydocalm) is a centrally acting muscle relaxant with few sedative side effects that is used for the treatment of chronic pain conditions. We describe three cases of suicidal tolperisone poisoning in three healthy young subjects in the years 2006, 2008 and 2009. In all cases, macroscopic and microscopic autopsy findings did not reveal the cause of death. Systematic toxicological analysis (STA) including immunological tests, screening for volatile substances and blood, urine and gastric content screening by GC-MS and HPLC-DAD demonstrated the presence of tolperisone in all cases. In addition to tolperisone, only the analgesics paracetamol (acetaminophen), ibuprofen and naproxen could be detected. The blood ethanol concentrations were all lower than 0.10 g/kg. Tolperisone was extracted by liquid-liquid extraction using n-chlorobutane as the extraction solvent. The quantification was performed by GC-NPD analysis of blood, urine and gastric content. Tolperisone concentrations of 7.0 mg/l, 14 mg/l and 19 mg/l were found in the blood of the deceased. In the absence of other autopsy findings, the deaths in these three cases were finally explained as a result of lethal tolperisone ingestion. To the best of our knowledge, these three cases are the first reported cases of suicidal tolperisone poisonings.     

Social learning and self-control: assessing the moderating potential of criminal propensity

Social learning theory is one of the most prominent general theories of crime. Yet recent research has called into question its applicability to all offenders. Specifically, the influence of antisocial peers has been found to exert a stronger effect among those individuals evincing higher levels of criminal propensity (deemed social amplification), whereas other components of the theory have either not been shown to interact with criminal propensity or not been tested. This study examines several social learning theory components to determine whether its influence is dependent on an individual's level of self-control. Results suggest little support for the social amplification hypothesis as the components of social learning theory were found to operate similarly across individuals regardless one's level of self-control. Implications for criminological theory are discussed.     

公证活动中证明责任的分配及转换

在公证活动中,对事实和权利的确认是以对支持事实和权利的证据的辨析和确认为前提的。而对证据的辨析和确认则是以证据的提供和获取为前提。公证证据收集是指在办理公证过程中,公证员依职责要求当事人提供证据或公证员依职责直接调取核实的证据的行为。公证活动中待证的事实有的发生于过去,而有的则发生在现在,加之公证活动参与主体的多元化和证据提供行为的主体性,使公证活动中不仅存在着证明责任,而且还涉及证明责任在主体之间分担问题。因此,证明责任的分配是公证证明活动的首要环节,是构建公证证明活动体系的框架性支柱。     

我国社会主义法治建设的路向展望——1982年宪法实施30周年的思考

1982年宪法实施30年来,我国社会主义法治建设取得了举世瞩目的成就。当前我们极有必要汲取30年来的得失,及时把握新时期我国社会主义法治建设的宏观走向。主要包括:我国社会主义法治建设应当融入善治理想,奠定善治基调;在顶层设计上坚持并规范党的领导,强化政党法治建设;在主题精神上,弘扬法治生活,让法治成为公民的生活方式。