Quantification of human mitochondrial DNA using synthesized DNA standards

Successful mitochondrial DNA (mtDNA) forensic analysis depends on sufficient quantity and quality of mtDNA. A real-time quantitative PCR assay was developed to assess such characteristics in a DNA sample, which utilizes a duplex, synthetic DNA to ensure optimal quality assurance and quality control. The assay's 105-base pair target sequence facilitates amplification of degraded DNA and is minimally homologous to nonhuman mtDNA. The primers and probe hybridize to a region that has relatively few sequence polymorphisms. The assay can also identify the presence of PCR inhibitors and thus indicate the need for sample repurification. The results show that the assay provides information down to 10 copies and provides a dynamic range spanning seven orders of magnitude. Additional experiments demonstrated that as few as 300 mtDNA copies resulted in successful hypervariable region amplification, information that permits sample conservation and optimized downstream PCR testing. The assay described is rapid, reliable, and robust.     

Globalisation with Growth and Equity: can we really have it all?

The purpose of US foreign assistance has shifted in the wake of 2001, and Washington has resurrected practices previously associated with police aid during the Cold War. In particular, the Bush administration has broadened the remit of the United States Agency for International Development (usaid) in such a way as to make it a quasi-security agency. The consequences of this could be significant for both usaid and democratic-style police assistance programmes more generally, for today's threat-driven policies are part of a trend which in the past has had worrying consequences. Using the critical variable of public policing (which is illustrated by reference to developments in Kenya), I argue that using usaid to improve the counter-terrorist capacity of Africa's police in the pursuit of US national security objectives is a seriously flawed strategy.     

How best to open up local democracy? A randomised experiment to encourage contested elections and greater representativeness in English parish councils

Interventions aimed at increasing the supply and representativeness of elected officials range from facilitative to the formally authorised. This paper reports on a field experiment aimed at testing the effect of facilitative approaches at the local level based on a collaboration between parish councils and the research team. We randomly allocated 818 parish council clerks across five counties in Southern England, either to receive information and the opportunity for member training for recruitment, or not to receive this contact. We investigated the effect of this intervention on political recruitment. Despite evidence of an effect on use of social media, our results suggest that there are significant institutional and structural barriers to participation in local politics that cannot easily be overcome using facilitative measures.