Ideological sources of American movements

He is retired and lives in Hawaii.     

An improved method for post-PCR purification for mtDNA sequence analysis

Mitochondrial DNA (mtDNA) analysis of forensic samples typically is performed when the quantity and quality of DNA are insufficient for nuclear DNA analysis or when maternal relatives may be the only reference source. Many of the steps required in the analytical process are both lengthy and labor intensive. Therefore, improvements in the process that reduce labor without compromising the quality of the data are desirable. The current procedure requires purification of the amplicons by centrifugal filtration after PCR and prior to cycle sequencing. Because this method requires several manipulations to perform, alternate cleanup procedures were investigated. These include the use of 1) Qiagen QlAquick PCR Purification columns, 2) Concert Rapid PCR Purification columns, and 3) ExoSAP-IT reagent. When the yield of purified amplicons, quality of the sequence profile, and ease of assay were evaluated, the use of ExoSAP-IT reagent for post-amplification purification was chosen to replace the filtration method.     

Conflict in the home environment of adolescents from divorced families: A longitudinal analysis

The purpose of this study was to examine conflict in the home environment of adolescents from divorced families across a 3-year period. Adolescents from intact (106) and mother-custody divorced (60) families participated. Interparental conflict and parent-adolescent relations were assessed. Results indicated that adolescents from divorced families did not experience more conflictual home environments than those from intact families. In fact, interparental relations after the first year and parent-adolescent relations in general appeared to be less conflictual in divorced families. Limitations and implications of the findings are discussed.