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Optimization and validation of a fully automated silica-coated magnetic beads purification technology in forensics 总被引:6,自引:0,他引:6
Nagy M Otremba P Krüger C Bergner-Greiner S Anders P Henske B Prinz M Roewer L 《Forensic science international》2005,152(1):13-22
Automated procedures for forensic DNA analyses are essential not only for large-throughput sample preparation, but are also needed to avoid errors during routine sample preparation. The most critical stage in PCR-based forensic analysis is DNA isolation, which should yield as much highly purified DNA as possible. The extraction method used consists of pre-treatment of stains and samples, cell lysis using chaotropic reagents, binding of the DNA to silica-coated magnetic particles, followed by elution of the DNA. Our work focuses mainly on sample preparation, obtaining the maximum possible amount of biological material from forensic samples, and the following cell lysis, to create a simple standardized lysis protocol suitable for nearly all forensic material. After optimization and validation, the M-48 BioRobot((R)) workstation has been used for more than 20,000 routine lab samples. There has been no evidence of cross contamination. Resulting DNA from as small as three nuclear cells yield reliable complete STR amplification profiles. The DNA remains stable after 2 years of storage. 相似文献
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234.
Anders Lidström 《Political studies》2006,54(4):865-888
Commuting has become an increasingly important feature of modern life. Theories of public participation, such as the civic voluntarism model, claim that commuting is likely to reduce the time available for political activism. Based on data from an American context, Robert Putnam in Bowling Alone has concluded that this is exactly what happens. However, empirical studies based on European data on how commuting may affect political participation are rare. This article aims to address this question with regard to Swedish city-regions. Is there also a negative relationship between commuting and citizen participation in Sweden?
The analysis is based on survey data for 7,200 citizens from seven Swedish city-regions belonging to three different size categories. The relationship between commuting and several different forms of public participation is investigated, controlling for the variables suggested by the civic voluntarism model. The analysis indicates that there are no signs of a negative relationship and some aspects of participation are actually positively linked to commuting. These findings suggest that the civic voluntarism model needs to be revised, at least in a European context. The article ends with a discussion about how differences between Sweden and the US can be accounted for and what the more general consequences for democracy may be. 相似文献
The analysis is based on survey data for 7,200 citizens from seven Swedish city-regions belonging to three different size categories. The relationship between commuting and several different forms of public participation is investigated, controlling for the variables suggested by the civic voluntarism model. The analysis indicates that there are no signs of a negative relationship and some aspects of participation are actually positively linked to commuting. These findings suggest that the civic voluntarism model needs to be revised, at least in a European context. The article ends with a discussion about how differences between Sweden and the US can be accounted for and what the more general consequences for democracy may be. 相似文献
235.
Torfinn Beer MD Anders Eriksson MD PhD Carl Johan Wingren MD PhD 《Journal of forensic sciences》2023,68(2):518-523
Fatal intoxications with opioids are known to be associated with an increased lung weight, as well as with brain and pulmonary edema and urinary retention. However, there is evidence to suggest that fatal intoxications with non-opioid substances are also associated with increased lung weight; however, the latter aspect has not been comprehensively analyzed. To determine to what extent opioid and non-opioid substances are associated with increased lung and brain weight, we studied these organs in cases where the cause of death was attributed to intoxication with a single agent. Using data from cases autopsied at the National Board of Forensic Medicine (NBFM) in Sweden from 2009 through 2019 where the cause of death was attributed to a single substance, we created models of combined lung weight and brain weight. The models used age and sex as predictors as well as nested varying effects for the specific intoxicant and category of intoxicant. Suicidal hanging with negative toxicology cases served as controls. The population majority was male among both intoxications (68%) and controls (83%). The most common single substance group was opioids. All tested substances were associated with heavier lungs than controls, with the largest effect in the opioid group. Our findings show that several substances are associated with increased lung weight and that among intoxication deaths there is no difference in expected brain weight between substances. Hence, heavy lungs, without a reasonable explanation, should prompt a broad toxicological screening. 相似文献
236.
Recent research has illustrated that demographic diversity influences the outcomes of public sector organizations. Most studies have focused on workforce diversity; by comparison, little is known about how managerial diversity affects organizational outcomes. This article focuses on gender diversity in the top management teams of public organizations and its relationship to financial performance. Theory suggests that management diversity can be a positive asset for organizations, allowing for the use of more diverse knowledge and human skill sets. Results of this study, however, suggest that organizations may only be able to leverage these advantages if they have a supporting management structure. In a longitudinal study of top management teams in Danish municipalities, the authors find that gender diversity in top management teams is associated with higher financial performance, but only in municipalities with a management structure that supports cross‐functional team work. These results are interpreted in light of existing theory, and implications are suggested. 相似文献
237.
This study analysed and determined factors associated with performance in collective action of a forest under participatory forest management in Southern Ethiopia. Data were collected in 22 user groups and a performance index was computed using indicators including forest cover change, households' perception of the change and satisfaction in their livelihoods, management activity, and length of overdue rent payment. The results show that heterogeneity, level of forest dependence and geographical variables such as altitude and distance to town are important variables that may affect the performance of user groups. The findings indicate the importance of taking into account the needs of members of user groups, differences among local people in dependence on forest income, and differences in values attached to the forest in the design of participatory forest management. 相似文献
238.
John Loughlin Professor of Politics Anders Lidstrom Chris Hudson 《Local Government Studies》2013,39(3):351-366
Local authorities have played an important role in the Swedish model of the Welfare State. This state is characterised by high levels of welfare provision paid for through general taxation, the rates of which are very high and the application of uniform standards across the entire country based on the principles of equity and fairness. The main form of income for local authorities is a Local Income Tax which is paid by 85% of the population. In the 1980s and 1990s, Sweden went through an economic crisis which resulted in significant changes to the tax system, although the Local Income Tax was retained. Subsequent changes have followed a pendulum process, with deregulation of local government finances in the 1990s being followed by greater regulation in the following years. Today, Sweden is conducting a major debate about the role and functions of different levels of government in the light of social, economic and political changes in the international scene. 相似文献
239.
Samantha Mann Aldert Vrij Dominic J. Shaw Sharon Leal Sarah Ewens Jackie Hillman Par Anders Granhag Ronald P. Fisher 《Legal and Criminological Psychology》2013,18(2):324-340
Background. We examined the effect of a second interviewer's demeanour on cues to deception. We predicted that a supportive demeanour would be the most beneficial for eliciting verbal cues to deceit, as it would encourage truth tellers, but not liars, to say more. In addition, we examined the extent to which interviewees deliberately made eye contact with the interviewers. Liars take their credibility less for granted than truth tellers, and therefore have a greater drive to be convincing. Liars are thus more likely to monitor the interviewer to determine if the interviewer appears to believe them. Method. Participants appeared before two interviewers: the first asked all the questions and the second remained silent. The second interviewer exhibited either a supportive, neutral, or a suspicious demeanour. Results. Truth tellers provided significantly more detail than liars, but only in the supportive second interviewer condition. The effect of a second interviewer's demeanour on detail was perhaps remarkable given that the interviewees hardly looked at the second interviewer (less than 10% of the time). Liars displayed more deliberate eye contact (with the first interviewer) than truth tellers did. Conclusions. A supportive second interviewer has a positive effect on interviewing. We discuss this finding in the wider contexts of investigative interviewing and interviewing to detect deception. 相似文献
240.