Comparison of ethyl glucuronide in hair with phosphatidylethanol in whole blood as post-mortem markers of alcohol abuse

Ethyl glucuronide (EtG) is a direct metabolite of ethanol and has been used as a marker of alcohol abuse in both urine and hair. This study investigated the value of EtG testing in post-mortem hair for diagnostic improvement of alcohol abuse in forensic medicine. Material from 70 consecutive medico-legal autopsies was collected in accordance with the recommendations on ethics by the Swedish National Board of Forensic Medicine. A method for determination of EtG in hair samples was developed using ultra performance liquid chromatography/electrospray tandem mass spectrometry (UPLC/ESI-MS/MS; LOQ, 2.5 pg/mg). The result of the EtG analysis was compared with the findings of phosphatidylethanol (PEth) in femoral whole blood, as measured by high performance liquid chromatography with an evaporative light-scattering detector (HPLC-ELSD; LOQ, 0.22 micromol/l). Evaluation of liver histology and anamnestic evidence of alcohol abuse of the deceased were taken in consideration for the interpretation. Measurable levels of EtG were present in 49 of the 70 autopsy cases whereas PEth was present in 36. Thirty-nine cases had EtG levels above the cutoff limit (> or = 30 pg/mg) compared with 29 for PEth (> or = 0.7 micromol/l). Fifteen cases had EtG as exclusive indicator for alcohol abuse compared with four cases for PEth. These findings suggest that measurements of EtG in hair may provide improved diagnostic information on alcohol abuse, due to a long retrospective time-window for detection and stability of EtG in hair in the decaying cadaver. However, an EtG level below the cutoff does not completely exclude previous alcohol abuse.     

Territorialising identity,authority and conflict in Africa: an introduction

In much of Africa, the simultaneous contestation over sub-national demarcations, political identities and the locus of authority has activated a territorial politics. In such contexts of uncertainty, state rulers and other social forces advance competing notions of the relevant and legitimate boundaries of territories and identities. This collection of articles examines how, across the continent, struggles over territory are linked to divergent understandings of identity and authority, with significance for territorial integrity, national identity and conflict.     

Tactical Voting in Norway: Context,Motives and Occurrence

Hitherto, tactical voting has not been a topic in Norwegian electoral research, despite the fact that tactical considerations have been publically discussed both by politicians and citizens for years. The complexity of the electoral system is partly to blame. Norwegian voters experience a number of tactical situations that give rise to rather different dilemmas, and hence several tactical motives. These need to be mapped and analysed separately. A set of survey questions has been especially designed for the present study to record these motives. Special attention has also been paid to the political sophistication of voters, campaign messages encouraging tactical voting, and the restraining effects of habitual voting and negative attitudes towards tactical voting. These factors may modify the inclination to tactical electoral behaviour. The web survey designed for the present project was conducted immediately following the Norwegian parliamentary election of 2013 (N = 2,278). Of the voters in the survey, 18.3 percent reported casting a tactically motivated vote. The 4 percent threshold on the distribution of compensatory seats, the competition for the last district seats and the composition of government coalitions triggered tactical voting. Tactical voters do not stand out as more politically sophisticated than other voters. Rather than calculating the expected utility for each party, they seem to rely on campaign information from the political parties and the media when voting tactically. For the habitual voters and voters with a strong dislike of tactical voting, the propensity for tactical voting is well below average.     

Unintentional Nonhunting Firearm Deaths in Sweden, 1983–2012

We examined the association between unintentional nonhunting firearm deaths and changes in firearm legislation in Sweden. There were 43 fatalities during the study time frame 1983–2012, representing 46% of all unintentional firearm deaths during the same period. The victims were predominantly young males (mean age 25 years). Slightly more than half of the deaths were caused by another person and were inflicted at close range. The main cause of the incidents was human error. The majority of the involved firearms were legal; however, most victims killed with illegal firearms were under the influence of alcohol and/or drugs at the time. The death rate decreased significantly following the introduction of the hunter's examination in 1985. Education and training associated with the hunter's examination was at least partially responsible for the decline in fatalities after 1985. Future prevention should target the availability of illegal firearms.     

Service development accounts for an even smaller share of European R&D investments than we may think

The Journal of Technology Transfer - In academic research on private R&D investments, sectoral differences are generally analyzed on the basis of firms’ classification according to...     

Y-STR DNA amplification as biological evidence in sexually assaulted female victims with no cytological detection of spermatozoa

Identification of spermatozoa is the biological evidence most often sought in specimens from rape victims. Absence of spermatozoa usually terminates biological investigations, and the victim's testimony can be contested. We assessed the utility and reliability of PCR amplification using Y-chromosomal STR polymorphisms in specimens from female victims of sexual assault with negative cytology.One hundred and four swabs without spermatozoa detected by cytology were collected from 79 alleged sexually assaulted female victims and amplification of Y-STR and of amelogenin was performed.Overall, Y-chromosome was detected and evidenced sexual penetration in 28.8% of swabs. In the population of victims examined more than 48 h after the sexual assault, Y-STR were still evidenced in 30% of the cases. These results show that swabs should be taken from victims for Y-chromosome DNA typing even after long delays between sexual assault and medical examination.     

Liquid chromatography/photodiode array detection for determination of strychnine in blood: a fatal case report

An original liquid chromatography method with photodiode-array detection (DAD) is presented for the determination of strychnine in blood. This sensitive method allows the use of only 0.1 ml of sample. The strychnine was isolated from blood using a liquid-liquid extraction procedure and chloroquine as an internal standard. The limits of detection (LOD) and quantification were 0.06 and 0.5 mg/l, respectively. The recovery was 94% and the coefficients of variation (CV) ranged from 5.9 to 10.8%. A fatal case of strychnine poisoning is presented, with a lethal blood concentration of 25 mg/l.