A duplex real-time qPCR assay for the quantification of human nuclear and mitochondrial DNA in forensic samples: implications for quantifying DNA in degraded samples

A duplex real-time qPCR assay was developed for quantifying human nuclear and mitochondrial DNA in forensic samples. The nuclear portion of the assay utilized amplification of a approximately 170-190 bp target sequence that spans the repeat region of the TH01 STR locus, and the mitochondrial portion of the assay utilized amplification of a 69 bp target sequence in the ND1 region. Validation studies, performed on an ABI 7000 SDS instrument using TaqMan detection, demonstrated that both portions of the duplex assay provide suitable quantification sensitivity and precision down to 10-15 copies of each genome of interest and that neither portion shows cross-reactivity to commonly encountered non-human genomes. As part of the validation studies, a series of DNase-degraded samples were quantified using three different methods: the duplex nuclear-mitochondrial qPCR assay, the ABI Quantifiler Human DNA Quantification Kit qPCR assay, which amplifies and detects a 62 bp nuclear target sequence, and slot blot hybridization. For non-degraded and moderately degraded samples in the series, all three methods were suitably accurate for quantifying nuclear DNA to achieve successful STR amplifications to yield complete profiles using the ABI AmpFlSTR Identifiler kit. However, for highly degraded samples, the duplex qPCR assay provided better estimates of nuclear template for STR amplification than did either the commercial qPCR assay, which overestimated the quantity of STR-sized DNA fragments, leading to an increased proportion of undetected alleles at the larger STR loci, or slot blot hybridization, which underestimated the quantity of nuclear DNA, leading to an increased proportion of STR amplification artifacts due to amplification of excess template.     

Drug smuggling using clothing impregnated with cocaine

A case study is presented where a woman travelling from South America to the Republic of Ireland was detained at Dublin Airport and articles of clothing she had in her luggage were found to be impregnated with cocaine. The study shows that the amount of powder recovered from the garments was approximately 14% of the total weight of the garments. The cocaine was in the form of cocaine hydrochloride and the purity was approximately 80%. An examination of the garments under filtered light highlighted the areas exposed to cocaine and indicated that the method of impregnation was by pouring liquid containing cocaine onto the clothing.     

Hedging Against China: Japanese Strategy Towards A Rising Power

Debates about how states deal with rising powers have been mainly concentrated on a continuum comprising on balancing and bandwagoning strategies. While theory has principally offered realist and liberal explanations, Japanese behavior vis-à-vis China does not match with them. Japan is not powerful enough to balance against China but remains too strong to bandwagon. Accordingly, Tokyo is pursuing a mixed strategy of both containment and engagement, which may be better described as a hedging strategy against Beijing. This article analyzes which strategies states can adopt when dealing with a rising power and proposes a framework to analyze Japan’s recent policy towards China based on Kuik’s analysis. We argue that Japan’s hedging strategy towards China is consistent with how middle-power states deal with rising power.     

Congresses versus caudillos: the untold history of democracy in Latin America,with special emphasis on New Granada (Colombia), 1830–60. A new research agenda

SUMMARY

Since their origins, congresses played significant roles in the emerging states of Latin America following independence from Spain. Yet their protagonism has been overshadowed by the so-called caudillos, the strongmen who seem to have dominated the politics of the region during most of the nineteenth century. This article argues that congresses were central political actors in Latin America during the century and it does so by examining their various functions. Congresses served to form governments, to define the legislative agenda and to limit the power of the executive. Congress was the institution around which political parties and their leaders were formed, while the practices of representative government developed.     

Militancy and parliamentary representation in Chile, 1849–79. Notes for a prosopographical study of the chamber of deputies

SUMMARY

This article provides a political and social characterization of Chilean deputies in the second half of the nineteenth century. It covers 11 parliamentary elections ranging from 1849, the first election under a relatively recognizable party system to 1879, the last election before the consequences of the War of the Pacific (1879–84) altered the local political landscape. The study of parliamentarians is based on a prosopography derived from the analysis of 405 deputies, and in this case is limited to three criteria: place of birth, profession or occupation, and age of entry into parliament. The latter criterion is a key one, because the authors focus only on members who joined congress for the first time over a period of 30 years. One of the main hypotheses of this article suggests that from the 1861 election congress experienced changes in its composition, allowing the entrance of actors who had hitherto not been part of congress.     

Lifestyle Preferences and Strategies of Spanish Working Mothers: A Matter of Choice?

There is a rich debate surrounding the causes of women’s heterogeneous work–life orientations and strategies. This article focuses on the debate between postmodern individualistic approaches and gender approaches that emphasise the pervasiveness of socio-structural factors. After offering a profile of Spanish women in the twenty-first century, we present the results of qualitative research based on 30 semi-structured interviews with working mothers from different social backgrounds. We discuss Hakim’s ‘preference theory’ by exposing how social class shapes Spanish working mothers’ work–life attitudes and behaviour and how these further change over the life cycle or as a result of changing contexts.     

Reviews

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