Highly reliable quantification of proteins such as members of the HSP70 superfamily based on the grey scale index via immune detection stained bands on a Western blot

The HSP70 superfamily is a reliable biomarker for hyperthermia, hypothermia and hypoxia. The Enzyme-linked Immunosorbent Assay (ELISA) respectively immunohistochemically staining methods are the typically used techniques for the quantification of those proteins. As the costs for reagents and devices as well as the work schedule of these methods are immense it was the goal of our study to develop an easy and reliable method to quantify the concentration of specific proteins. We established a procedure to measure the relative concentration of proteins fixed on ROTI(?) PVDF membranes via Western blot, calculating the relative protein concentration in dependency to the grey scale index of the normalized and digitalized pictures of the bands on the blots.     

Adult-onset antisocial behavior trajectories: associations with adolescent family processes and emerging adulthood functioning

Guided by conceptual and empirical work on emerging adulthood, this study investigated the role of closeness to mother and father and behavioral autonomy during adolescence on the development of adult-onset antisocial behavior. Using data from the National Longitudinal Study of Adolescent Health (Add Health), we identified four aggressive (abstainer, adolescent-limited, adult-onset, chronic) and three nonaggressive (adolescent-limited, adult-onset, chronic) trajectories. Members of the aggressive adult-onset trajectory reported higher levels of paternal closeness during adolescence compared to the members of the aggressive chronic trajectory. Maternal closeness and behavioral autonomy did not differentially predict trajectory membership. In addition, members of the adult-onset trajectories were less likely to be employed or in a romantic relationship and reported decreased physical health during emerging adulthood compared to members of all other trajectories.     

Stranger rape: classifying Spanish sexual offences using multiple correspondence and cluster analyses

We have analysed the information in 342 police reports of stranger sexual offences recorded in 2010. We have carried out a multiple correspondence analysis and a cluster analysis using modus operandi variables to identify differential profiles in these types of sexual offences. We have come up with three profiles of stranger sexual offences, which concur in the two techniques used. By analysing the personal variables of the offenders with such profiles, we have found differences in terms of the offender’s country of origin and age. We will discuss the consequences of these results on the police investigation of stranger sexual offences.     

Economic crisis and lawmaking. The impact of crisis on legislative agenda in Italy

Major economic crises are focal events that often drive changes in various aspects of political systems. Although extensive work has been done to investigate the effect of exogenous shocks on political phenomena such as government termination, public opinion and policy outcomes, the impact of major crises on the process of policymaking has so far received scarce attention. Building on existing literature on policy agendas and legislative organization, this paper explores how the Eurozone crisis has affected the legislative agenda of the Italian parliament. The data used include information on the 1,110 bills submitted to parliament during Legislature XVI (2008–2013). Our analysis shows that, with the worsening of the crisis, bill proposals related to macroeconomic issues become increasingly more likely to enter the legislative agenda, displacing legislation dealing with other topics. Our argument is corroborated by a comparison between Legislature XVI and a pre-crisis legislature (2001–2006), as in the latter term the legislative agenda follows different patterns.     

Patent-based investment funds: from invention to innovation

In the context of entrepreneurial ecosystems the transformation of inventions to innovations is a crucial measure of success. Yet, the exploitation of inventions stemming from academic or corporate research is not as extensive as we would wish leaving room for improvements. Drawing on the knowledge spillover theory of entrepreneurship we investigate the phenomenon of patent-based investment funds as a new type of intermediary in the knowledge spillover process, which could facilitate the transformation from invention to innovation. Using a qualitative research design we analyze data from 21 expert interviews and complementary archival data. We find common characteristics of funds’ activities which decrease knowledge filters and fill the financing gap in the early stages of technology development. We propose a classification of commercialization strategies and link them to a specific set of invention characteristics. Our insights contribute to the knowledge spillover theory of entrepreneurship and to the knowledge filter model by providing empirical evidence for the division of labor between knowledge creator and commercialization agent. This adds to our view on entrepreneurial ecosystems as we shed light on different players in the transition process from invention to innovation and thus enhance our understanding of the multifaceted aspects in such an ecosystem. In addition, we refine the literature on patent-based investment funds by providing a classification of the entire commercialization spectrum used by funds and add to extant theorizing on how the nature of a technology determines its commercialization.

     

Choosing Healthplans All Together: a deliberative exercise for allocating limited health care resources

CHAT (Choosing Healthplans All Together) is an exercise in participatory decision making designed to engage the public in health care priority setting. Participants work individually and then in groups to distribute a limited number of pegs on a board as they select from a wide range of insurance options. Randomly distributed health events illustrate the consequences of insurance choices. In 1999-2000, the authors conducted fifty sessions of CHAT involving 592 residents of North Carolina. The exercise was rated highly regarding ease of use, informativeness, and enjoyment. Participants found the information believable and complete, thought the group decision-making process was fair, and were willing to abide by group decisions. CHAT holds promise as a tool to foster group deliberation, generate collective choices, and incorporate the preferences and values of consumers into allocation decisions. It can serve to inform and stimulate public dialogue about limited health care resources.     

Perianal and vulvar Crohn's disease presenting as suspected abuse

Misdiagnosis of sexual abuse may arise in cases of vulvar and/or perianal diseases such as lichen sclerosus et atrophicus, Behcet's syndrome, bullous diseases, contact dermatitis, or neoplastic lesions. We present the case of a 7-year-old girl who was referred by her general paediatrician to the local hospital for perianal fissures and swelling of the labia majora. A report to the judicial authorities was made, for suspected sexual abuse, and the patient was transferred to the department of paediatric surgery. Here, histopathologic examination of vulvar and rectal biopsies revealed multiple non-caseating and non-confluent epithelioid-gigantocellular granulomas, consistent with a diagnosis of Crohn's disease, with cutaneous involvement of the genitalia. In cases of suspected sexual abuse, examination of children should be performed by a specialist in legal medicine in collaboration with a gynaecologist or paediatric surgeon. If the patient is hospitalised and the question of protection does not arise, physicians should exclude dermatological diseases before reporting to the judicial authorities.     

Medical study: aspiring parents, genotypes and phenotypes: the unexamined myth of the perfect baby

Although many have argued that assisted reproductive technologies ("ARTs") attract those with a desire to genetically engineer their offspring, this claim has yet to be verified. To address this question, we surveyed three groups: the general public, people enrolling in an in vitro fertilization ("IVF") program, and pregnant couples. We asked subject which traits they would select in their children if it were possible to use a magic wand to do so and to value genetic relatedness. In our sample, the potential parents who were using ARTs were less likely to express a desire to select traits in their offspring than were the general public, and just as likely as the pregnant couples, Those using ARTs , however, place greater importance on having genetically related children than the others. Thus, the widely held view that reproductive technology is utilized by those most likely to favor genetic engineering is falsified by out findings.     

Predictions of Future Dangerousness in Capital Murder Trials: Is It Time to “Disinvent the Wheel?”

Although recent U.S. Supreme Court decisions regarding the death penalty (e.g., Atkins v. Virginia, 2002) have renewed interest in mental health issues, one topic that has not received much attention recently is the ongoing use of expert testimony to support claims that defendants represent a continuing threat to society. In this article, we (a) review prior research relevant to determining the accuracy of clinical predictions that capital defendants will commit future acts of criminal violence; (b) summarize new data from current and former death row inmates in Texas that bolster the claim that such predictions are gross overestimates of risk; and (c) review extant research addressing the potential utility of various risk assessment instruments that increasingly are being used to reinforce clinical predictions in capital trials. Despite significant recent advances in the field of risk assessment, clinical assertions that a defendant is likely to commit future violent acts appear to be highly inaccurate and ethically questionable at best. Moreover, available research offers little support for the claim that the accuracy of these predictions will be appreciably improved by relying on more structured risk assessment measures that have some demonstrated predictive validity in other contexts.Portions of this article were written while the first author was a member of the Department of Psychology at Sam Houston State University. The prison inmate data reported in this study also are described in a report by the Texas Defender Service, available at: