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661.
Ann Marie Gross Amy A Liberty Megan M Ulland Jacquelyn K Kuriger 《Journal of forensic sciences》2008,53(1):125-134
Y-chromosomal short-tandem repeat (Y-STR) amplification has been used in forensic casework at the Bureau of Criminal Apprehension (BCA) Forensic Science Laboratory since 2003. At that time, two separate amplifications were required to type the SWGDAM recommended loci (DYS19, DYS385a/b, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS438, and DYS439). The Yfiler kit coamplifies these loci as well as DYS437, DYS448, DYS456, DYS458, DYS635, and Y GATA H4. The Yfiler kit was validated following the internal validations outlined in the SWGDAM revised validation guidelines. Our studies show that 0.125 ng of male DNA will generate a complete 17 locus profile and that as little as 0.06 ng of male DNA yields an average of nine loci. In the male-male mixtures, a complete profile from the minor component was detected up to 1:5 ratio; most of the alleles of the minor component were detected at a 1:10 ratio and more than half the alleles of the minor component were detected at a 1:20 ratio. Complete YSTR profiles were obtained when 500 pg male DNA was mixed with female DNA at ratios up to 1:1000. At ratios of 1:5000 and 1:10,000 (male DNA to female DNA) inhibition of the YSTR amplification was evident. The YSTR results obtained for the adjudicated case samples gave significantly more information than the autosomal results. Our studies demonstrate that the Yfiler kit is extremely sensitive, does not exhibit cross-reactivity with female DNA, successfully types male DNA in the presence of overwhelming amounts of female DNA and is successful in typing actual forensic samples from adjudicated cases. 相似文献
662.
663.
Collaboration Processes: Inside the Black Box 总被引:6,自引:1,他引:5
Social science research contains a wealth of knowledge for people seeking to understand collaboration processes. The authors argue that public managers should look inside the "black box" of collaboration processes. Inside, they will find a complex construct of five variable dimensions: governance, administration, organizational autonomy, mutuality, and norms. Public managers must know these five dimensions and manage them intentionally in order to collaborate effectively. 相似文献
664.
Ann Nevile 《Australian Journal of Public Administration》2000,59(2):63-74
This article explores the major influences on pay‐TV policy‐making in Australia from the late 1970s, when the issue was first discussed in the context of proposals to establish a domestic communications satellite, up until the introduction of pay‐TV in Australia in 1995, thereby placing current developments in the pay‐TV industry in context. The article argues that among larger broadcasting and telecommunications issues, pay‐TV has never been ‘the main game’. For both Coalition and Labor governments, the expansion of existing free‐to‐air television services in regional Australia and the introduction of competition in telecommunications have been much more important policy priorities than the introduction of pay‐TV. Furthermore, because pay‐TV policy‐making largely took place in this wider policy context, the structure of the broadcasting and telecommunications industries also shaped pay‐TV policy outcomes. 相似文献
665.
Juries, criminologists, prosecutors, law enforcement officials, and the lay public like to know the motive for a murder—especially when there are unusual features such as multiple victims, extensive injuries, or unexplainable acts to the bodies. However, many times the motive is not readily available from the murderer because he does not confess or he does not consciously know why he killed. The following case describes the analysis of multiple motives in a triple spree homicide committed in a very short time frame. Robbery was the primary motive for the first victim and sexual homicide was the motive for the second and third victims. 相似文献
666.
Pam Willson Judith McFarlane Ann Malecha Dorothy Lemmey 《Journal of Police and Criminal Psychology》2001,16(1):40-50
To evaluate the perceived effectiveness of domestic violence assistance offered by a special unit of an urban police department,
90 women having filed assault, stalking, or harassment charges on an intimate were interviewed. Threats of abuse, physical
abuse, and stalking were measured—86% of the women reported experiencing severe abuse and 60% of the women had been stalked.
On follow-up interview—41% of the women reported the abuse had stopped, while 7% said the abuse had decreased. Half of the
women had used information provided by the unit, with 86% of the women considering police services received as being helpful.
Authors' Note: Pam Willson, Ph.D., is a post-doctoral research associate at Texas Woman's University in Houston. Judith McFarlane, Dr.
PH, currently holds the Parry Chair in Health Promotion and Disease Prevention at the University. Ann Malecha, Ph.D., is assistant
professor of nursing at the University. Dorothy Lemmey, Ph.D., is associate professor of Nursing at Lakeland College. 相似文献
667.
668.
Ann Marie Gross M.S. ; Amy A. Liberty M.S. ; Megan M. Ulland B.S. ; Jacquelyn K. Kuriger M.S. 《Journal of forensic sciences》2008,53(1):125-134
Abstract: Y-chromosomal short-tandem repeat (Y-STR) amplification has been used in forensic casework at the Bureau of Criminal Apprehension (BCA) Forensic Science Laboratory since 2003. At that time, two separate amplifications were required to type the SWGDAM recommended loci (DYS19, DYS385a/b, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS438, and DYS439). The Yfiler™ kit coamplifies these loci as well as DYS437, DYS448, DYS456, DYS458, DYS635, and Y GATA H4. The Yfiler™ kit was validated following the internal validations outlined in the SWGDAM revised validation guidelines. Our studies show that 0.125 ng of male DNA will generate a complete 17 locus profile and that as little as 0.06 ng of male DNA yields an average of nine loci. In the male–male mixtures, a complete profile from the minor component was detected up to 1:5 ratio; most of the alleles of the minor component were detected at a 1:10 ratio and more than half the alleles of the minor component were detected at a 1:20 ratio. Complete YSTR profiles were obtained when 500 pg male DNA was mixed with female DNA at ratios up to 1:1000. At ratios of 1:5000 and 1:10,000 (male DNA to female DNA) inhibition of the YSTR amplification was evident. The YSTR results obtained for the adjudicated case samples gave significantly more probative information than the autosomal results. Our studies demonstrate that the Yfiler™ kit is extremely sensitive, does not exhibit cross-reactivity with female DNA, successfully types male DNA in the presence of overwhelming amounts of female DNA and is successful in typing actual forensic samples from adjudicated cases. 相似文献
669.
Ann Woodward 《Public administration review》2009,69(1):25-28
In "Beyond Cognition: Affective Leadership and Emotional Labor," Professors Newman, Guy, and Mastracci further the discussion of contemporary leadership by exploring leadership transitions in public agencies that serve vulnerable populations, such as the abused elder, the neglected child, or the court-involved teenager. 相似文献
670.
Ann Reyes Robbins 《Juvenile & family court journal》2008,59(1):3-15
This Essay considers the emerging research in the area of dual‐jurisdiction children, often referred to as “crossover kids”—those currently or previously involved in maltreatment proceedings who have also committed delinquent acts. Part I describes the development of the juvenile courts in the early twentieth century. Part II of this Essay questions the need to “track” children along one legal path or another and points to the pitfalls of providing services to some children through a criminal justice paradigm instead of treating all children through a social work paradigm. Finally, Part III advocates a redesign of the juvenile court—a return to its roots—to better enable a court to consider the needs of the whole child, in context with the needs of her/his family. 相似文献