A novel fluorescence-based method in forensic science for the detection of blood in situ

Full DNA profiles can be generated from just a few cells; however these profiles can be contaminated from other cell types present at the crime scene. We report here on the development of an immunofluorescent technique to spatially locate human-specific blood in situ and also on the ability of this technique to detect individual leukocytes and the DNA contained within them. Four monoclonal mouse anti-human antibodies were evaluated; anti-glycophorin A to detect erythrocytes and anti-CD45, anti-myeloperoxidase (MPO) and anti-histone H1 to detect the nucleated leukocytes. Each antibody was labeled with either Alexa Fluor 488 or 568 for direct application to blood smears which allowed the simultaneous detection of erythrocytes and leukocytes. Furthermore, because histones are DNA binding proteins, the application of anti-histone H1 allowed the detection of DNA within a blood smear. Importantly it was found that full DNA profiles could be achieved after using this method with similar peak area ratios compared to untreated cells. The fluorescent antibodies were found to be human-specific with the exception of anti-histone H1 due to its conserved sequence. However, used in combination with anti-CD45 or anti-MPO the location of DNA from human-specific leukocytes could be detected. The technique was also tested on older blood stains and was still found to be sensitive and cell-specific after 4 months. Following the optimization of the methodology, the fluorescent antibodies were applied to short lengths of black cotton fibres covered with human blood spots. Although the background fluorescence from the cotton was found to be high, erythrocytes and even individual leukocytes could easily be detected, indicating that this technique could be used to detect extremely minute amounts of blood. Used in combination with laser capture microdissection (LCM), this method could be used to pick off individual leukocytes for LCN DNA techniques.     

Digital Restructuring: Gender,Class and Citizenship in the Information Society in Canada

‘Digital restructuring’ denotes a phenomenon integral to but also distinct within economic and political restructuring broadly conceived. The concept of restructuring can be modified with ‘digital’ to forefront the important technological dimension of global restructuring, as well as to indicate developments associated with the new ‘information economy’. Digital technology and digitization have been integral to the scope and speed of the global economic and political restructuring of recent decades. They have constituted the technological conditions for some of the more characteristic aspects of this process; from the flexiblization or outright shedding of labour, to the mobility of production and capital and the globalization of trade and financial markets. This paper seeks to debunk much of the corporate and state mythology of digital restructuring currently in circulation by drawing upon the analyses of digital technology and restructuring advanced by critical scholars and progressive social movements, and to highlight the dangers to progressive political movements and discourses posed by the very nature of these representations.     

AN EXPLORATORY STUDY OF SUPERVISED ACCESS AND CUSTODY EXCHANGE SERVICES

An exploratory outcome study to investigate the ability of a supervised access and custody exchange center to function as a safe visitation enforcement mechanism of the court was accomplished as part of a larger study investigating child well-being. During a 6-month period of program participation, frequency and consistency of noncustodial parents' access to children dramatically increased and interparental conflict significantly decreased, demonstrating that supervised visitation and custody exchange centers that function in partnership with family court during interim court processes can address the unmet needs of family court and high-conflict domestic disputant as well.     

Histoire/histoires: special issue in honour of claire duchen

Claire Duchen died on 15 March 2000 after a long and very brave struggle with ovarian cancer. She died aged 45, but already with an established reputation as a leading scholar of French feminism. As Siân Reynolds wrote in her obituary for The Guardian newspaper, Claire was central to a global network of feminist scholars as a writer, translator, research coordinator, and for over 10 years, from 1987–1997, regional editor for Africa and Europe of Women's Studies International Forum. Her particular areas of specialism, in which she published widely were French Studies, especially French feminisms on which she published two well-known books, Women's Studies, and contemporary French and European Women's History, on which she published her last book this year.² She was respected as a scholar and valued as a colleague and a friend by many women and men around the world.     

Literatur

Ohne Zusammenfassung     

Impacts of abstinence education on teen sexual activity, risk of pregnancy, and risk of sexually transmitted diseases.

This paper examines the impacts of four abstinence-only education programs on adolescent sexual activity and risks of pregnancy and sexually transmitted diseases (STDs). Based on an experimental design, the impact analysis uses survey data collected in 2005 and early 2006 from more than 2,000 teens who had been randomly assigned to either a program group that was eligible to participate in one of the four programs or a control group that was not. The findings show no significant impact on teen sexual activity, no differences in rates of unprotected sex, and some impacts on knowledge of STDs and perceived effectiveness of condoms and birth control pills