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821.
822.
When Abusive Head Trauma (AHT) is suspected in a child, a dilated eye examination by an ophthalmologist is an essential part of the medical workup, as the presence and pattern of retinal hemorrhages can have a high positive predictive rate for abusive head injury. This article proposes to review the clinical presentation, pathophysiology, natural history, sequelae, and differential diagnosis of retinal hemorrhages and other ocular lesions associated with AHT. 相似文献
823.
When cases of suspected abusive head trauma are adjudicated in courts of law, one of the theories often presented by defense experts is that a normal, healthy infant or child suddenly neurologically deteriorates or dies several weeks to months after birth or minor injury because a preceding subdural hematoma spontaneously rebleeds after a minor traumatic event. This article reviews the underlying scientific basis of subdural hematoma rebleeding as it applies to this courtroom theory. 相似文献
824.
Richard Thorogate Joana C.S. Moreira Sue Jickells Margherita M.P. Miele Barbara Daniel 《Forensic Science International: Genetics Supplement Series》2008,2(4):363-371
Full DNA profiles can be generated from just a few cells; however these profiles can be contaminated from other cell types present at the crime scene. We report here on the development of an immunofluorescent technique to spatially locate human-specific blood in situ and also on the ability of this technique to detect individual leukocytes and the DNA contained within them. Four monoclonal mouse anti-human antibodies were evaluated; anti-glycophorin A to detect erythrocytes and anti-CD45, anti-myeloperoxidase (MPO) and anti-histone H1 to detect the nucleated leukocytes. Each antibody was labeled with either Alexa Fluor 488 or 568 for direct application to blood smears which allowed the simultaneous detection of erythrocytes and leukocytes. Furthermore, because histones are DNA binding proteins, the application of anti-histone H1 allowed the detection of DNA within a blood smear. Importantly it was found that full DNA profiles could be achieved after using this method with similar peak area ratios compared to untreated cells. The fluorescent antibodies were found to be human-specific with the exception of anti-histone H1 due to its conserved sequence. However, used in combination with anti-CD45 or anti-MPO the location of DNA from human-specific leukocytes could be detected. The technique was also tested on older blood stains and was still found to be sensitive and cell-specific after 4 months. Following the optimization of the methodology, the fluorescent antibodies were applied to short lengths of black cotton fibres covered with human blood spots. Although the background fluorescence from the cotton was found to be high, erythrocytes and even individual leukocytes could easily be detected, indicating that this technique could be used to detect extremely minute amounts of blood. Used in combination with laser capture microdissection (LCM), this method could be used to pick off individual leukocytes for LCN DNA techniques. 相似文献
825.
Barbara Haak Andrea Porsche Kai Vollack Peter Zimmermann Werner Pflug 《Forensic Science International: Genetics Supplement Series》2008,1(1):35-36
In order to cope with the demanding workload for DNA profiling of forensic casework samples a concept for a semi-automated processing system was developed at the Landeskriminalamt (Office of Criminal Investigation) Baden-Württemberg, Germany [K. Vollack, et al., Implementation of a semi-automated processing system for DNA profiling of forensic casework samples, this issue]. The applied magnetic bead extraction method is based on ChargeSwitch® Technology (CST) from Invitrogen and was established on a liquid handling workstation Freedom EVO® 150 from Tecan. 相似文献
826.
The new intramarital distributions of 190 young parents in their third month of first parenthood were studied in a multifaceted distributive and procedural justice framework. For a large majority, justice was an important dimension in their intramarital distributions of household labor, child care, leisure time, breadwinning. Justice ratings of various principles in general were more liberal than the reported practice, in women even more than in men. The practice reported was mainly traditional, with a gender-segregated distribution of labor. A pattern of marked consistencies and inconsistencies between the principles rated as just and factual practice emerged. Inconsistencies occurred more often in women than in men. 相似文献
827.
Barbara Potthast-Jutkeit 《The History of the Family》2013,18(2):115-121
The historical study of the family started later in the “Third World” than in Europe and North America, and the link between colonialism and family structures in the colonized or formerly colonized countries has not been explored thoroughly. In this issue we have chosen examples from Africa, Latin America, and the Caribbean in part because the importance of people of African background in both continents provided a certain link. The main connection, though, was the fact that all examined cultures had to cope with Christian-European family norms and with the values of the colonizers. The aims, forms, and historical circumstances of the colonial situation were quite different in all the cases examined. When changing conditions made traditional kinship bonds less reliable or less workable, people turned to alternative institutions such as gynaegamy or gender groups. If there is one conclusion to be drawn from all the examined cases, it is that colonized societies had the ability to use a variety of family forms as they adjusted to new situations. 相似文献