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271.
Stewart JE Aagaard PJ Pokorak EG Polanskey D Budowle B 《Journal of forensic sciences》2003,48(3):571-580
Laser-induced detection of fluorescent labeled PCR products and multi-wavelength detection (i.e., multicolor analysis) enables rapid generation of mtDNA sequencing profiles. Traditionally, polyacrylamide slab gels have been used as the electrophoretic medium for mtDNA sequencing in forensic analyses. Replacement of slab gel electrophoresis with capillary electrophoresis (CE) can facilitate automation of the analytical process. Automation and high throughput can be further enhanced by using multicapillary electrophoretic systems. The use of the ABI Prism 3100 Genetic Analyzer (ABI 3100, Applied Biosystems, Foster City, CA) as well as the ABI Prism 310 Genetic Analyzer (ABI 310, Applied Biosystems, Foster City, CA) were evaluated for mtDNA sequencing capabilities and compared with sequencing results obtained on the platform currently in use in the FBI Laboratory (the ABI Prism 377 DNA Sequencer, ABI 377, Applied Biosystems, Foster City, CA). Various studies were performed to assess the utility of the ABI 3100, as well as the ABI 310 for mtDNA sequencing. The tests included: comparisons of results obtained among the ABI 3100, the ABI 310 and the ABI 377 instruments; comparisons of results obtained within and between capillary arrays; evaluation of capillary length; evaluation of sample injection time; evaluation of the resolution of mixtures/heteroplasmic samples; and evaluation of the sensitivity of detection of a minor component with reduced template on the ABI 3100. In addition, other studies were performed to improve sample preparation; these included: comparison of template suppression reagent (TSR, Applied Biosystems, Foster City, CA) versus formamide; the use of Performa DTR Gel Filtration Cartridges (Edge BioSystems Inc., Gaithersburg, MD) versus Centri-Sep Spin Columns (Princeton Separations, Adelphia, NJ) for product purification after cycle sequencing; and sample stability after denaturation. The data support that valid and reliable results can be obtained using either capillary electrophoresis instrument, and the quality of sequencing results are comparable to or better than those obtained from the ABI 377 instrument. 相似文献
272.
Preliminary research using on-line supercritical fluid extraction/gas chromatography-mass spectrometry (SFE/GC-MS) has shown that the natural and artificial surface components of human scalp hair are reproducible and differentiable. Therefore, these components may be useful for individualization or determining demographic characteristics or both. However, it is not known how the efficiency and selectivity of on-line SFE/GC-MS compares to other extraction methods. In this study, ultrasound, Soxhlet, and pressurized-fluid extraction were used to extract 1 mg to 1.3 g portions of a composite hair sample taken from an Asian male between the ages of 10 and 18. Percent extractables ranged from 0.9% to 5.6%, depending on the solvent used, and tended to increase with solvent polarity. Chemical analysis using GC/MS showed that the extracts contained large proportions of free fatty acids, squalene, cholesterol, and various wax esters. Finally, comparisons to SFE/GC-MS showed that this method possesses adequate efficiency, no observable differences in selectivity, and greater potential for miniaturization. 相似文献
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International Journal for the Semiotics of Law - Revue internationale de Sémiotique juridique - 相似文献
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Four sets of reforms of the National Health Service are employed to illustrate the changing character of policy making in this sector over a thirty year period, from the production of a carefully developed technocratic blueprint for its organization to the promulgation of a series of bright ideas accompanied by incentives for local actors to develop them into concrete organizational arrangements consonant with these ideas. We term this latter approach ‘manipulated emergence’ and relate it to the literatures of organizational culture and of post-Fordism. The approach adopted by the 1997 Labour government is largely, though not wholly consistent with this, and it remains to be seen whether the high-water mark of manipulated emergence has passed. 相似文献
276.
Population displacements in the aftermath of the Chechen conflict may undermine a complex political balance existing among the extraordinarily numerous ethnic groups in the neighbouring Republic of Dagestan. The destabilisation of Dagestan threatens to bring ethnic conflict to virtually the only state in the Caucasus to have avoided it thus far. This article considers the basis of ethnic accommodation in Dagestan, the policies and migratory patterns that have placed it at risk, and the regional context in which these have occurred. 相似文献
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Multidimensional scaling analysis was used to examine adolescents' cognitive appraisals of major and daily stressful events. The desirability of events was the only salient feature for early adolescents. Middle and late adolescents also appraised events in terms of their desirability; in addition, the amount of impact that events exerted on their lives and the generality of the causes of events were salient dimensions for these age groups. These results suggest increased complexity with age in adolescents' cognitive appraisals of stressful events. Correlational analyses also indicated that perceptions of personal coping capabilities are associated with high event desirability, high frequency of occurrence, stable causes, and decreased ability of others to be of help in coping.This research served as a portion of the doctoral dissertation of the first author and was supported in part by an institutional award from the University of Vermont to the second author.Received his Ph.D. from University of Vermont. Research interests include stressful events during adolescence.Assistant Professor of Psychology at the University of Vermont where he is investigating stress and coping among children and adolescents. Received his Ph.D from UCLA in 1980. 相似文献