The development of reduced size STR amplicons as tools for analysis of degraded DNA

New multiplex PCR sets of commonly used short tandem repeat (STR) markers have been developed to produce PCR products that are reduced in size when compared to standard commercial STR kits. The reduction in size of these amplicons can facilitate the examination and analysis of degraded DNA evidence by improving amplification efficiency. This "miniSTR" approach will permit current forensic practitioners to use STR markers and instrumentation already present in their laboratories and to generate genotyping data that is directly comparable to reference samples and searchable through the FBI's Combined DNA Index System (CODIS) databases. This paper discusses the development of these new primer sets and presents some initial results in the analysis of degraded and aged DNA samples. A method for removal of problematic fluorescent dye artifacts is also described. Comparison studies in over 100 samples have verified that these miniSTR primers can provide fully concordant results to commercial STR kits and can provide improved signal from degraded DNA specimens. These miniplex sets should prove valuable in the analysis of samples where allele dropout and reduced sensitivity of larger STR alleles occurs.     

Reapportionment: A study in comparative government

The periodic redrawing of electoral boundaries is a necessity in any equitable single-member representative system. It is approached in very different ways in the United States and in Britain. In America, against a background of continuous intervention by the courts, party politics and legislative fixing are endemic. Britain (and the other established democracies of the Commonwealth) have all turned increasingly to neutral commissions that are in theory (and, on the whole, in practice) blind to considerations of party advantage. This article explores how this contrast has developed. The reasons behind the profound differences in procedure offer unexpectedly rich insights into comparative government.     

When marketing models clash with democracy

The application of marketing models in political and public sector contexts is examined. The assumptions in marketing of positive outcomes of (i) rapid responses to consumer concerns, (ii) the extension of choice and customisation in product development, and (iii) the application of market research techniques are considered in turn. This analysis suggests that in the political context, responding rapidly to public opinion is not necessarily a sound reaction; extending choice and customisation of products may not best serve public welfare, and applying market research techniques may not provide for the best system for policy decisions. The features of liberal representative democracy, particularly the role of deliberation, informed assent and accountability, have been neglected. Speed of response has been emphasised to the cost of democratic filters and checks on public opinion; enhanced choice, enabled by mass customisation, presents problems of social fragmentation; and the application of market research is no substitute for political discourse and engagement. Copyright © 2003 Henry Stewart Publications     

The Spreading of Innovation: State Adoptions of Energy and Climate Change Policy

The American states are engaged in a variety of policy efforts to mitigate climate change and alter energy usage. While a number of studies have considered the reasons for adoption of renewable energy and climate change policies, they typically consider only one policy in isolation. This study examines policy adoption of 14 energy and climate change programs in a pooled event history analysis. Our primary research questions consider average effects of horizontal policy diffusion, while also identifying factors that vary across policy type. We offer a method of testing whether predictors vary across policies and use this test to incorporate interactions by policy in the statistical analysis. Our results indicate that many of the primary drivers of adoption are political in nature, including state ideology, environmental interest group membership, and diffusion via ideologically similar neighbors. In addition, we find that given policy heterogeneity, a number of determinants vary by policy type, though differences are in magnitude rather than direction.     

Uses of the NIST 26plex STR assay for human identity testing

Ongoing work at the U.S. National Institute of Standards and Technology has focused on the characterization of 26 autosomal STR loci for human identity testing. These 26 loci are in addition to the existing 13 U.S. core loci and those found in PowerPlex16 and Identifiler commercial STR typing kits. The amplification of the 26 loci has been optimized for degraded extracts in unique miniplex panels and also for reference samples as a single reaction 26plex assay. A study has been performed comparing genotypes obtained with the 26plex primers to those with miniplex panels for allele drop out and concordance. The forensic utility of the 26plex assay was evaluated for situations where additional loci are beneficial. The utility of this large multiplex was also tested in a case involving DNA extracted from degraded bone samples. The 26plex can serve as a low-cost assay (compared to commercially available kits) useful for both sorting comingled remains and providing additional markers for increased statistical support for samples that require “non-trio” family references for human identification.     

Rethinking Prison Disciplinary Processes: A Potential Future for Restorative Justice

Abstract

The movement for restorative justice (RJ) has struggled with marginalization on the soft end of the criminal justice system where the threat of net widening and iatrogenesis looms large. To realize the full potential of RJ as an alternative philosophy of justice, restorative practices need to expand beyond the world of adolescent and small-level offenses into the deeper end of the justice system. Disciplinary hearings inside of adult prisons may be a strategic space to advance this expansion. This article presents findings from a study of prison discipline in four U.K. prisons. The findings strongly suggest that in their current form such disciplinary proceedings are viewed by prisoners as lacking in legitimacy. Although modeled after the adversarial system of the criminal court, the adjudications were instead universally derided as “kangaroo courts” lacking the basic elements of procedural justice. Based on these findings, we argue that RJ interventions may offer a viable redress to these problems of legitimacy which, if successful, would have ramifications that extend well beyond the prison walls.     

Antisocial Beliefs and Attitudes in Pre-adolescent and Adolescent Youth: the Development of the Antisocial Beliefs and Attitudes Scales (ABAS)

A focus on antisocial beliefs and attitudes has informed both the assessment and treatment literature, and practice, in youth justice service provision. This study attempts to broaden the construct of antisocial beliefs and attitudes and reports on the psychometric development of the Antisocial Beliefs and Attitudes Scale (ABAS). The ABAS is a new instrument for measuring antisocial thinking in older children and adolescents, which is developmentally-sensitive, broad-based, and assesses domains grounded in the empirical literature on childhood conduct problems and delinquency. Along with a self-reported measure of antisocial behavior, the ABAS was administered to 425 school children aged 10–18 (M = 14.18; SD = 2.31). A meaningful factor structure emerged from our instrument with two of the three factors, Rule Non-Compliance and Peer Conflict, predicting self-reported antisocial behavior while demonstrating sensitivity to age and gender. These findings are encouraging in our aim to “bridge” the measurement gap, by developing a reliable and valid measure of antisocial thinking applicable to older children and adolescents.