西尼罗病毒E蛋白的原核表达及其多克隆抗体的制备

利用PCR技术扩增西尼罗病毒(WNV)E蛋白基因并将其插入原核表达载体pET-30a(+),构建重组质粒pET-30a(+)-WNV-E,并转化至大肠杆菌BL21(DE3)感受态细胞中,经IPTG诱导目的蛋白表达,对诱导条件进行优化,利用His镍柱亲和层析法纯化目的蛋白,纯化后的蛋白经SDS-PAGE与Westernblot鉴定正确后,免疫BALB/c小鼠制备多克隆抗体并进行间接免疫荧光鉴定。结果显示:WNV E蛋白在大肠杆菌中以包涵体形式被成功表达,蛋白最佳诱导条件为37℃,0.8 mmol/L IPTG诱导5 h,经间接免疫荧光鉴定多克隆抗体能特异性识别WNV E蛋白。本研究成功表达并纯化了WNV E蛋白,证明该蛋白具有良好的免疫原性,为WNV抗原、抗体检测方法的建立和新型疫苗的研发及相关研究奠定了基础。     

新冠疫情防控常态化下加强创新集体协商工作的思考

由于国外新冠肺炎疫情的快速蔓延以及国内疫情的起伏,使得我国新冠疫情防控呈现出常态化和长期化的趋势。后疫情时期,构建和谐的企业劳动关系,助力企业复工复产和健康发展是工会组织的当务之急。集体协商制度是市场经济国家构建和谐企业劳动关系的根本制度和主要手段,工会组织要积极引导劳动关系双方在集体协商制度框架下主张各自的权利和寻求平衡点。     

Rechtliche Einordnung von Grünbrücken bei Aus- und Neubauten sowie bestehenden Bundesfernstraßen

Natur und Recht - Angesichts der enormen Zerschneidungswirkung von Bundesfernstraßen werden Grünbrücken in der Bundesrepublik vermehrt errichtet, sodass sich die Frage der...     

改制企业与劳动者的确认劳动关系纠纷属民事诉讼受案范围——以谭艮生诉明达公司、石柱县制鞋厂确认劳动关系纠纷案为例

确认劳动关系纠纷是平等主体之间的民事争议,具有民事可诉性,属于人民法院民事诉讼的受案范围。无论是政府及其所属主管部门主导的企业改制还是企业自主改制,均不改变发生在改制企业与劳动者之间的确认劳动关系纠纷的民事争议属性,也不改变其民事可诉性。因此,发生在改制企业与劳动者之间的确认劳动关系纠纷,亦应属于人民法院民事诉讼的受案范围。     

Discrimination of phenethylamine regioisomers and structural analogues by Raman spectroscopy

In this study, the Raman spectra of 21 phenethylamines were obtained using far‐red excitation (785 nm). The distinguishing ability of Raman for phenethylamines, especially for phenethylamine regioisomers and structural analogues, was investigated. Here, the evaluation of a cross section of Raman spectra demonstrated that all types of phenethylamines were distinguishable, even for certain structural analogues with high spectrum similarity. Raman exhibited high distinguishing ability for phenethylamine regioisomers that differ in the substitution position of halogen, methoxy, alkyl, or other substituted groups; as well as for structural analogues containing different groups, such as furanyl, 2,3‐dihydrofuranyl, halogen, and alkyl substituted at the same position. The Raman spectra for homologues with differences in only a methyl group were found to be highly similar; however, their spectra demonstrated small but detectable differences. Four analogue mixtures and 59 seized samples were also analyzed to study the practical use of the Raman method in forensic field. 95% of the seized samples were correctly identified, which significantly validated the ability of Raman method in identifying the correct isomers. Accordingly, this study provides a non‐destructive, high‐throughput and minimal sample preparation technique for the discrimination of phenethylamines.     

社区治理共同体建设——新时代社区治理制度化的理论逻辑与实现路径

社区治理共同体是社会治理共同体的基础单元,社区治理共同体建设的制度化程度直接关乎社会治理现代化的制度化水平和总体质量.进入新时代,推动社区治理共同体的制度建设,必须坚持和完善党对社区治理工作的全面领导,发挥党组织的政治引领、组织引领、能力引领、机制引领作用.同时,构建政府负责下简约高效的基层社会管理体制,通过政府向社会力量购买服务机制实现社区公共服务的社会协同供给,以民主协商的方式实现社区治理的公众参与.还要以法治为保障、以科技为支撑,提升和推动社区治理和服务的法治化水平和信息化建设,进而建设人人有责、人人尽责、人人享有的社区治理共同体.     

Fiscal slack or environmental pressures: which matters more for technological innovation assimilation? A configurational approach

Abstract

Prior studies suggest that fiscal slack may positively affect technological innovation in the public sector but yield inconsistency between theoretical expectations and empirical findings. To address this research gap, this study develops a configurational explanation combining fiscal slack and environmental pressures by employing fs-QCA in the context of Chinese provincial governments’ online public service provision. The findings reveal that the role of fiscal slack is environmentally dependent on explaining technological innovation and that environmental pressures seem to be more important. That is, fiscal slack is likely irrelevant to a high degree of technological innovation assimilation, while the demanding environments of multiple environmental pressures matter. Relative fiscal scarcity may be somewhat relevant to a low degree of technological innovation assimilation only when combined with a more relaxed environment. Several theoretical and practical implications are discussed.