Correlation of methamphetamine results and concentrations between head, axillary, and pubic hair

This study was designed to compare the qualitative results and concentrations of methamphetamine (MA) and its metabolite amphetamine (AP) in head hair and hair collected from different parts of the body (axillae and pubis). Hair from subjects (N = 14) suspected MA users was simultaneously collected. Hair preparation involved washing step, fine cutting, overnight extraction, derivatization by the trifluoroacetic anhydride, and gas chromatography/mass spectrometry (GC/MS) using selective ion monitoring. In this study, we found a good correlation of the qualitative results for MA between head hair and hair on other parts of the body, but there were some differences in concentrations of MA and AP. Namely, the concentrations of MA and AP were higher in axillary and pubic hair than in head hair.     

Population data for the AmpFl STR Identifiler PCR Amplification Kit in China Han in Jilin Province, China

Allele frequencies for the fifteen short tandem repeats (STR) loci D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D7S820, D19S433, THO1, D16S539, CSF1PO, TPOX, D2S1338 (AmpFl STR Identifiler PCR Amplification Kit, Applied Biosystems) were determined in a population sample of unrelated China Han in Jilin Province, China. Statistical analyses were performed using Powerstats version 1.2 and GEP-ISFGWG. The results showed that all the loci met Hardy-Weinberg expectation.     

The prevalence of MDMA/MDA in both hair and urine in drug users

The prevalence and age distribution of 3,4-methylenedioxymethamphetamine (MDMA) and 3,4-methylenedioxyamphetamine (MDA) in hair samples by gas chromatography/mass spectrometry (GC/MS) were studied. The recoveries obtained from hair were 97% and 99% for MDMA and MDA, respectively. The inter- and intra-assay precision and accuracy were determined. Out of 791 hair samples, 44 (5.6 %) contained MDMA and/or MDA. Out of these 44 subjects, urinalyses from 35 were negative for both MDMA and MDA, while only 9 were positive. We also evaluated concentrations of MDMA and MDA, and the metabolite-to-parent drug ratios. This study showed that the abuse of MDMA or MDA was found principally among young adults and male abusers. We found the epidemiology of ecstasy users in Korea between March 2002 and April 2003.     

Study on the application of parent-of-origin specific DNA methylation markers to forensic genetics

In paternity test, especially in motherless cases, the allele inherited from father (obligatory gene, OG) often cannot be determined. The paternity exclusion probability (PE) of a genetic marker is reduced considerably. Therefore, it is necessary to develop a new technique, by which the parental origin of alleles can be determined without genealogical analysis. In this paper, we explored the possibility of using parent-of-origin specific DNA methylation markers to determine the parental origin of alleles, choosing the imprinted single nucleotide polymorphism (SNP) locus rs220028 (A/G) as a model system. We typed the SNP by mutagenically separated PCR (MS-PCR). The frequencies of alleles were A = 0.5085, G = 0.4915; the unbiased heterozygosity was 0.5020. In order to discriminate between the maternal allele and paternal allele, post-digestion MS-PCR, a novel PCR based methylation analysis and SNP typing technique was developed and performed on 18 heterozygous children, and the methylated maternal allele was detected specifically. As a pilot study on the use of epigenetic markers in forensic genetics, our results demonstrated the feasibility of using parent-of-origin specific DNA methylation markers to determine the parental origin of alleles.     

从统战角度看中国素朴民本思想

统战须做人的工作,应了解中国素朴民本思想。其合理立意,“君民”互动,“信”“诚”“礼”“刑”与“民”之关系,民本的目的性、主体性,“君”“民”区别对待等理论对统战都有启示、借鉴意义。     

论城市牛皮癣的综合治理

"城市牛皮癣"不仅影响了城市环境的美观整洁,严重污染了市容市貌,也使一些制贩假证件、假文凭、私刻假印章、兜售假发票等不法活动日益猖獗,扰乱了正常的社会秩序,侵害了国家、集体的公共利益和有关单位的合法权益。要彻底根除"城市牛皮癣",还都市靓丽形象,必须标本兼治,综合运用行政、法律、舆论、教育等手段,动员城管、公安、电信、市民等一齐动手,举全社会之力,打一场围剿"城市牛皮癣"的"人民战争"。     

公安院校“两课”教学的困境与改革探索

公安院校"两课"教学在学生政治思想素质形成过程中发挥着重要作用,但也还存在许多不足之处.通过对云南警官学院"两课"教学的调研,发现"两课"教学的现存问题.本文拟从改进"两课"教学的基本方法进行探索,以达到提高"两课"教学效果为目的.     

试论“砸碎三个锁链”思想到“三个文明”建设思想的发展

150多年前,革命导师马克思提出了"失去的是锁链,得到的将是整个世界"的论断,这一论断具有丰富的内容.如何完成无产阶级的历史使命,砸碎政治、经济和精神三个锁链,这既是一个理论性问题,又是一个实践性问题,为此,各国无产阶级开始了各自的探索.中国共产党提出的"三个文明"建设的思想,是对马克思"砸碎三个锁链"思想的丰富和发展.     

以人为本理念在公安"窗口"规范化建设实践中的运用

公安"窗口"是人民警察执法为民的前沿阵地.近年来,由于各级领导的高度重视与狠抓落实以及广大民警的共同努力,"窗口"规范化建设成效显著.但我们在检查中也发现了存在的一些问题.公安机关要强化以人为本的理念,有效推进"窗口"的规范化建设,以不断提高人民群众的满意率.     

论“其他组织”在我国民事法律主体体系中的地位

“其他组织”是我国法的特有概念 ,它现实是且应当是法定的第三民事主体。应当在我国民事基本法的总则编主体制度中对其作出规定。基于其与法人 (组织 )的对立统一 ,应改称为“非法人组织”。非法人组织的民事责任 ,应根据其目的上的营利性与否 ,进行区别对待