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A totally automated procedure has been developed for the detection and quantitation of morphine and codeine in urine case samples. The samples were initially screened for these drugs by a Syva EMIT Toxicology System (ETS). A Zymate laboratory robotic system confirms positive samples from Syva ETS by performing the hydrolysis, extraction, and derivatization of morphine and codeine. The derivatized morphine and codeine were detected using gas chromatography/mass spectrometry (GC/MS). Enzymatic hydrolysis conditions were experimentally optimized during method development. The automation of these procedures has proven to be reliable and efficient. 相似文献
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Maeda H Fujita MQ Zhu BL Yukioka H Shindo M Quan L Ishida K 《Forensic science international》2002,127(1-2):82-87
We report a possibly first forensic autopsy case of death following a spontaneous recovery from cardiopulmonary arrest (CPA) after clinical declaration of death: 'Lazarus phenomenon'. A 65-year-old male with congenital deafness and dumbness was found unconscious in his room at a public home. During pre-hospital and clinical resuscitation including defibrillation and medications for about 35 min, CPA persisted under electrocardiographic (ECG) monitoring and therefore, his death was pronounced. However, about 20 min later, a police officer who had been called for the postmortem investigation found the patient moving in the mortuary. The patient subsequently showed typical ECG signs and laboratory findings of early inferior wall myocardial infarction and died 4 days later. The forensic autopsy, due to alleged medical negligence, revealed myocardial infarction with thrombotic occlusion of the right coronary artery and secondary hypoxic brain damage. The present case and the related clinical literature suggest that, especially in cases of acute myocardial infarction in elderly patients, a careful observation to confirm death after discontinuation of resuscitation is recommended to provide appropriate medical care, irrespective of the quality or duration of advanced life supporting efforts. 相似文献
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Robert Turrisi James Jaccard Susan Q. Kelly Christina M. O'Malley 《Journal of youth and adolescence》1993,22(2):147-169
The present research examined factors that could be used to improve campaigns geared toward having adolescents prevent their friends from driving while intoxicated. Three areas were examined: (1) adolescents' ability to make accurate judgments of their friends' drunkenness using information about the number of drinks consumed and the time to consume, (2) their perceptions of the consequences that could ensue if they were to attempt to prevent their friends from driving while intoxicated, and (3) their knowledge of viable strategies if they were to attempt such interventions with their friends. The results show that adolescents have perceptual biases when using information about number of drinks and time to consume when making judgments of drunkenness, and that intervention attempts with friends are likely to result in confrontations. The implications of these findings for the timing and content of educational efforts is discussed.This research was supported in part by Grant AA0687502 from the National Institute of Alcohol Abuse and Alcoholism, and by the Center for Applied Research at the State University of New York at Albany.Received Ph.D. from University at Albany. Research interest is in social and cognitive psychology.Received Ph.D. from University of Illinois. Research interest is in social and cognitive psychology.Research interest in developmental psychology.Received B.S. from Siena College. Research interest is in social and cognitive psychology. 相似文献
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In situ hybridization with Digoxingenin labeled probes were used to build the relationship between incised cutaneous wounding intervals and the expression of splicing fibronectin variants (total, E III A and E III B Variants) in rats. The results showed that all forms of FN variants increased notably with the wounding time going on. E III A and E III B Variants were expressed abundantly 30 and 60 minutes after injury, while few in normal skin. 相似文献
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