适配体技术研究现状与法庭科学应用展望

适配体技术是生物科技研究中的新兴发展技术之一,具有广阔的应用前景。本文从适配体技术的发展、研究方法、特性以及应用领域进行综述,并对该技术在法庭科学中的应用做出展望,以推动我国法庭科学领域对适配体技术的研究和应用。     

法医学二代测序STR分型准确度与测序深度的关联性评估

目的利用实验数据对法医学二代测序STR分型测序深度与分型结果准确度的关联性进行评估。方法使用商业化基因组DNA制备单一来源和混合的DNA样本,以Thermo Fisher公司的25重早期测试试剂盒进行目的STR片段扩增,每种扩增产物分别使用4种不同的序列标签平行建库,并控制标记每一种序列标签的文库上机量依次占一张Ion 318芯片的1/4、1/8、1/16、1/32。经Ion PGMTM基因测序仪测序,以及Ion Torrent SuiteTM软件进行数据分析;同时对庞敬博等人发表的基于相同试剂盒和测序仪检测的95名中国汉族无关个体的6928条等位基因、影子峰和噪音序列进行测序深度统计分析,寻找测序深度与STR分型准确度的关联性。结果各基因座测序深度随文库上样量减少而呈明显下降趋势。对于单一来源样本,每张芯片上样不超过8个均一化文库可实现全部基因座的完整分型;对于1∶20比例的混合DNA,每张芯片上样不超过4个均一化文库时,未发现微量组分的等位基因丢失。人群数据测序深度统计显示,该体系基因座间存在不均衡性,有必要针对各基因座分别设定分析阈值参数。结论测序深度与法医学STR分型结果的准确性密切相关,各基因座最低测序深度与平均测序深度的比值可作为设定分析阈值的重要参考指标。本研究确定的单张芯片上样数量仅适用于本实验体系,但相关实验设计和方案可供其他实验体系开展类似工作参考。     

Managing policy‐making in the local climate governance landscape: The role of network administrative organizations and member cities

Trans‐municipal networks (TMNs) have reshaped the landscape around local government action on global climate policy. Past research has focused on why cities join TMNs and the impact that membership has on local action. This study considers a potential reverse effect: namely, that cities' membership choices position them to influence TMNs' overall priorities. In considering this, we emphasize the role of network administrative organizations (NAOs) and posit that the multiple climate‐related networks, which share members and operate in overlapping geographic and issue spaces, are bound together as part of a meta‐network. We utilize social network analysis and data on membership in four climate‐related TMNs to examine the factors that shape how cities can influence meta‐network priorities. We find that cities with local vulnerabilities tend to be located in network positions that enable them to influence the meta‐network's overall priorities.     

VNTR D17530位点多态性在亲子鉴定中的应用

运用微量热启动PCR技术,对20例正常家系的遗传学分析,证实D17530位点的遗传符合孟德尔遗传规律,表现为简单的共显性遗传。同时,对100例亲子鉴定案例进行回顾性分析研究,证明D17S30位．在多态性可应用于我国法医学亲子鉴定。根据D17S30位点基因频率估算的排除概率（74．04％）与其实际排除能力（80．00％）无显著差异。在15倒排除亲子关系的案例中,有2例由D17S30位点单独取得排除证据。D17S30位点是法医学上有重要意义的遗传学标记,可用于亲子鉴定。     

Polymorphism analysis of D6S366 STR locus

The polymorphic STR locus D6S366 was analyzed using PCR and PAG electrophoresis and 7 alleles were detected from 200 unrelated individuals in Wuhan. The allete distribution of the locus was in accordance with Hardy-Weinberg equilibrium. The H, Dp, P, E, and PIC of the D6S366 were calculated in this paper.     

Participatory numbers for integrating knowledge and actions in development

This article discusses the opportunities and challenges associated with the use of quantitative participatory methods and participatory numbers for integrating locals’ and outsiders’ knowledge, as well as actions from the bottom-up and top-down, in development. It places emphasis on the contribution of quantitative participatory methods and participatory numbers to foster dialogue between a wide range of stakeholders, for example, local people, NGOs, government agencies and scientists, who usually seldom directly engage with each other. The article draws on case studies that discuss remittances and disasters in Samoa, and disaster risk reduction in the Philippines.     

浅论对侦查行为的法律监督

我国现行侦查程序缺乏有效的法律监督．检察院对侦查程序的提前介入没有立法上的保障,法院无权对侦查机关的行为进行审查和控制,犯罪嫌疑人无权与侦查机关进行平等的对话和抗争。因此,从基本理念和基本结构上对我国侦查程序进行反思,有必要限制侦查机关的权力,保护被追诉方的权利,并赋予法院对侦查行为的司法审查权。     

浅析不动产预告登记制度——兼论我国预告登记制度的完善

预告登记是起源德国普鲁士法的一项古老制度,其旨在保护以不动产物权变动为目的的债权请求权,因其在保障不动产交易安全、促进交易效率等方面发挥了非常重要的作用,后被许多国家和地区采用。我国于2007年10月1日施行的《中华人民共和国物权法》正式确立了不动产预告登记制度,但是它在实践中还存在许多不足,亟待解决。     

论马克思自然概念的环境伦理学意蕴

文章认为,将马克思主义理解为人类中心主义,并批评马克思对自然的态度,是对马克思主义与人类中心论的双重误解;文章分析了马克思自然概念的相关论述,阐明了马克思自然概念的双重含义,澄清了在人本主义自然观视野中人与自然的辩证统一的关系;人与自然辩证统一关系思想超越了传统的人类中心论与西方环境伦理学的非人类中心论,为认识与构建人与自然的和谐关系奠定了基础。以此为基础,本文初步呈现了马克思环境伦理思想的核心观点,为推进中国的环境伦理学奠定哲学基础。