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111.
A collaborative exercise was carried out by the Spanish and Portuguese ISFG Working Group (GEP-ISFG) in order to evaluate the performance of two Y-chromosome STR PCR tetraplexes, which include the loci DYS461, GATA C4, DYS437 and DYS438 (GEPY I), and DYS460, GATA A10, GATA H4 and DYS439 (GEPY II). The participating laboratories were asked to type three samples for the eight markers, using a specific amplification protocol. In addition, two control samples, with known haplotypes, were provided. The results obtained by the 13 different participating laboratories were identical, except for two laboratories that failed to type correctly the same two samples for GATA C4. By sequence analyses, two different GATA C4 allele structures were found. One control sample (allele 21) and two questioned samples (allele 22, correctly typed by all the laboratories, and allele 25) presented the following repeat structure: (TCTA)4(TGTA)2(TCTA)2(TGTA)2(TCTA)n, but different from the one found for allele 26 in one sample included in this exercise, as well as in the second control sample (allele 23), namely (TCTA)4(TGTA)2(TCTA)2(TGTA)2(TCTA)2(TGTA)2(TCTA)n. The collaborative exercise results proved that both Y-tetraplexes produce good amplification results, with the advantage of being efficiently typed using different separation and detection methodologies. However, since GATA C4 repeat presents a complex structure, with alleles differing in sequence structure, efficient denaturing conditions should be followed in order to avoid typing errors due to sizing problems.  相似文献   
112.
Allele frequencies for the 15 autosomic STR loci included in the PowerPlex 16 System kit (Promega) were estimated from a sample of 111 unrelated individuals living in Neuquen province, southwest of Argentina. Population showed to be in HWE.  相似文献   
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A large literature has studied the trend of greaterpolarization between Democrats and Republicans in Congress.This paper empirically examines the extent to which inflationand unemployment explain cyclical movements ofpolarization over time. An informal application of thestandard Downsian spatial competition model of partiesgenerates the following relationships, ceteris paribus: (1)inflation should be associated with policy convergence, (2)unemployment should be associated with polarization, (3) theeffect of unemployment on polarization should be larger inmagnitude than the effect of inflation on convergence, and (4)the effect of unemployment on polarization should be strongerin the House than in the Senate. We estimate the relationshipbetween vote records and business cycle conditions over the1947–1999 period using a GLS model with varying lags. Ourresults are broadly consistent with these business cyclehypotheses of polarization, though greater support is found inHouse data than in Senate data.  相似文献   
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This paper examines theeffect of political competition in localelections in regional allocation of publicinvestment. The study employs data onMexican elections covering the period 1990–95, characterisedby an increase in electoral competition and coupled withincreasing demands for decentralisationthroughout the states. Empirical evidencesupports the hypothesis that regionalallocation of public investment by centralgovernment was driven by `politicalopportunism' and `local pork-barrelpolitics'. A positive relationship wasfound between the regional allocation ofpublic investment and support for thecentral ruling party. This might indicatethat local spending inefficiencies werepartially explained by the specific supportfor the incumbent party.  相似文献   
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This article examines the circumstances under which civilians, using protests as a mechanism, alter the strategic use of violence by armed actors (rebels and state forces). By examining the civil war in Colombia between 1988 and 2005, this study finds that combatants decrease their attacks against the population when civilians protest against the enemy. Combatants interpret such demonstrations as costly signals of loyalty. Furthermore, when insurgents are the target of the protests, insurgents increase repression against civilians as rebels get stronger. In contrast, state forces (and paramilitaries) compensate for their weakness in the area by multiplying civilian victims. Both state forces and rebels, however, are likely to decrease violence against civilians when civilians protest against both parties in contested zones. In such contexts, armed actors are likely to refrain from retaliation because any violence might drive noncombatants toward the enemy.  相似文献   
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Development cooperation is a foreign policy tool marked by deep-seated conflicts of interest and dilemmas of particular relevance to second-tier and non-nuclear countries that aim to change their international status and role. Building on the concept of ‘graduation dilemma’, this article compares specific dilemmas that Brazil and South Africa face in their foreign policies concerning the development cooperation agenda at three levels: the domestic, the regional and the global level. The research question guiding this analysis is: how does the graduation dilemma manifest in relation to Brazil and South Africa’s role in development cooperation?  相似文献   
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Drawing on four years of fieldwork in Ethiopia and Uganda, this paper addresses gaps in knowledge about the mechanisms linking agricultural exports with poverty reduction, the functioning of rural labour markets, and the relevance to the lives of the poorest people of Fairtrade. Statistical analysis of survey evidence, complemented by qualitative research, highlights the relatively poor payment and non-pay working conditions of those employed in research sites dominated by Fairtrade producer organisations. We conclude that Fairtrade is not an effective way to improve the welfare of the poorest rural people.  相似文献   
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