Short tandem repeat (STR) genotyping of keratinised hair. Part 2. An optimised genomic DNA extraction procedure reveals donor dependence of STR profiles

A feasibility study of short tandem repeat (STR) genotyping of telogen phase hairs in particular, and hair shaft in general, is presented. A number of extraction procedures in common use were investigated and the quantities of nuclear DNA (nuDNA) delivered were quantified via a real-time PCR assay. The extracts were subjected to two variations on AmpFlSTR Profiler Plus PCR amplification strategies (extended cycles, two rounds of PCR) and the genotypes compared. Nuclear DNA was found to persist in human hair shafts, albeit at very low levels. Full Profiler Plus profiles consistent with the hair donor were obtained from 100 mg hair shaft samples (bleached and unbleached). These were, however, mixed profiles, indicating low copy number (LCN) contamination in the extracts. Single telogen hair clubs and single hair shafts delivered partial profiles with usually only one allele of heterozygous loci. Telogen phase hairs yielded the same amount of nuDNA (and no more) as hair shafts (either anagen or telogen). Whether hair shafts dissolved or not in lysis buffer had no effect on either the quantitated yield of DNA or on the chance of obtaining a correct genotype. These results provide evidence that genomic DNA resides on the exterior of the hair shaft and we use this information to suggest an optimal procedure for nuDNA extraction from keratinised hair samples: soaking hairs in simple digestion buffers containing Tris-HCl, a salt and a chelating agent without prior cleaning of the hair shafts. It is proposed that cleaning removes most of the recoverable DNA. This procedure was applied to obtain genotypes from 3 cm hair shafts which matched reference profiles from the donors at up to 9 out of 10 AmpFlSTR Profiler Plus STR loci. When the genotyping success was measured by counting the number of matches between the two dominant alleles at each locus for each extract with the reference DNA profile of the hair donor, the success was found to be highly dependent on the donor. The number of matching alleles varied between not less than 10 for one donor to no more than two for another donor. These results may well be linked to the environmental experience of the hairs from each donor prior to removal.     

Criminal and civil community-based "detention": some parallels

Over the last 20 years, governments in Australia and elsewhere have increasingly embraced the notion of community-based treatment of the mentally ill in preference to detention in a mental health facility. At the same time, governments have also embraced the notion of community-based treatment and punishment of criminal offenders in preference to detention in a custodial facility. This article examines the use of Community Treatment Orders (CTOs) within the Victorian mental health regime, and the use of Intensive Correction Orders (ICOs) within the Victorian criminal justice regime. It is argued that a number of striking similarities can be found in the respective legislative schemes and policy considerations.     

Acculturation, partner violence, and psychological distress in refugee women from Somalia

This study examined the relations among acculturation, domestic violence, and mental health in 62 married refugee women from Somalia. Refugees from Somalia constituted the largest group of refugees entering the United States in 2005, and little is known about the presence of domestic violence in this group. The results showed that women who reported greater ability to speak English also reported more experiences of partner psychological abuse and physical aggression. Experiences of more psychological abuse and physical aggressions also predicted more psychological distress. Implications for future research and psychological services are addressed.     

Police Empathy and Victim PTSD as Potential Factors in Rape Case Attrition

This exploratory study investigated whether rape victims’ subjective perceptions of whether to proceed with legal action were associated with their experience of disclosing to the police during their initial interview. Specifically, the study investigated associations between symptoms of PTSD, shame and self-blame post-rape, subjective perceptions of police empathy and subjective perception of victims’ intentions to take the case to court. Participants (N = 22) were found to have elevated levels of PTSD severity, shame and self-blame. Police empathy was positively correlated with victims’ ratings of likelihood of taking the case to court, and negatively correlated with PTSD severity and shame. These preliminary findings suggest that training police officers how to respond more empathically to psychologically distressed rape victims may potentially help reduce victim attrition rates.     

China's Exceptionalism and the Challenges of Delivering Difference in Africa

This article explores the notion of ‘China's exceptionalism’ in Africa, a prominent feature in Beijing's current continental and bilateral engagement. ‘China's exceptionalism’ is understood as a normative modality of engagement that seeks to structure relations such that, though they may remain asymmetrical in economic content they are nonetheless characterised as equal in terms of recognition of economic gains and political standing (mutual respect and political equality). This article considers the burden that the central Chinese government has assumed through its self-construction and mobilisation of a position of exceptionalism and, concurrently, the imperatives that flow from such rhetorical claims of distinctiveness in terms of demonstrating and delivering difference as a means to sustain the unity and coherence of these rhetorical commitments.