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271.
Kacinko SL Barnes AJ Kim I Moolchan ET Wilson L Cooper GA Reid C Baldwin D Hand CW Huestis MA 《Forensic science international》2004,141(1):41-48
Oral fluid is an interesting alternative matrix for drug testing in many environments, including law enforcement, workplace drug testing, and drug treatment facilities. Performance characteristics of the FDA-cleared, qualitative, Cozart RapiScan Opiate Oral Fluid Drug Testing System (Opiate Cozart RapiScan System or Opiate CRS) were compared to the semi-quantitative Cozart Microplate EIA Opiate Oral Fluid Kit (Opiate ELISA) and to gas chromatography/mass spectrometry (GC/MS). The following oral fluid opiate cutoffs were evaluated: the GC/MS limit of quantification (LOQ) of 2.5 mg/l; 15 microg/l currently used for oral fluid testing in the United Kingdom (UK); 30 microg/l (Opiate CRS cutoff); and 40 microg/l, the proposed Substance Abuse and Mental Health Services Administration (SAMHSA) cutoff. Subjects provided informed consent to participate in this IRB-approved research and resided on the closed research ward throughout the study. Three oral codeine doses of 60 mg/70 kg were administered over a 7-day period. After a 3-week break, subjects received three doses of 120 mg/70 kg within 7 days. Oral fluid specimens (N = 1273) were analyzed for codeine (COD), norcodeine (NCOD), morphine (MOR) and normorphine (NMOR) by GC/MS with an LOQ of 2.5 microg/l for all analytes. MOR and NMOR were not detected in any sample; 26.5% of the specimens were positive for COD and 13.7% for NCOD. Opiate CRS uses a preset, qualitative cutoff of 10 microg/l; this is equivalent to 30 microg/l in undiluted oral fluid as the oral fluid collection process involves a 1:3 dilution with buffer. Sensitivity, specificity, and efficiency of Opiate CRS compared to Opiate ELISA were 98.6, 98.1, and 98.2% at a 30 microg/l cutoff and 99.0, 96.2, and 96.6% at a 40 microg/l cutoff. Compared to the much lower GC/MS LOQ of 2.5 microg/l, sensitivity, specificity and efficiency were 66.8, 99.3 and 90.7%. Increasing the GC/MS cutoff to the current UK level yielded performance characteristics of 81.5% (sensitivity), 99.3% (specificity), and 95.4% (efficiency). Using a GC/MS cutoff identical to the preset Opiate CRS cutoff yielded sensitivity, specificity, and efficiency of 88.5, 99.2, and 97.5%, respectively. At the proposed SAMSHA confirmation cutoff of 40 microg/l, sensitivity increased with little change in specificity and efficiency (91.3% sensitivity, 98.9% specificity, and 97.5% efficiency). Oral fluid is a suitable matrix for detecting drugs of abuse. Opiate CRS, with a 30 microg/l cutoff, is sufficiently sensitive, specific and efficient for oral fluid opiate analysis, performing similarly to Opiate ELISA at the same cutoff, and having performance characteristics >91% when compared to GC/MS at the proposed SAMHSA cutoff. 相似文献
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Successful extraction of human genomic DNA from serum and its application to forensic identification
Ravard-Goulvestre C Crainic K Guillon F Paraire F Durigon M de Mazancourt P 《Journal of forensic sciences》2004,49(1):60-63
We report here on the successful extraction of human genomic DNA from a serum sample in a forensic case. The extracted DNA was successfully used for the identification of remains presumably immersed for more than three weeks for which the only comparison sample was a 250-microL serum aliquot kept frozen in a laboratory. The analysis made it possible to identify a second victim as the daughter of the first. 相似文献
275.
Expert Decision Making in Burglars 总被引:1,自引:0,他引:1
276.
This article examines the sequential, temporal, and interactional aspects of sexual assaults using sequential analysis. Fourteen statements taken from victims of bedroom-based assaults were analyzed to provide a comprehensive account of the behavioral patterns of individuals in sexually charged conflict situations. The cases were found to vary in the sexual severity of assault, distinguishing a variety of motivations and behavioral repertoires of offender and victim. Two quite distinct styles of offense were identified: multiple and single, which may have very different implications for research into rapist taxonomies and rape prevention strategies. 相似文献
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Over the last decade, legal recognition of same-sex relationships in Canada has accelerated. By and large, same-sex cohabitants are now recognised in the same manner as opposite-sex cohabitants, and same-sex marriage was legalised in 2005. Without diminishing the struggle that lesbians and gay men have endured to secure this somewhat revolutionary legal recognition, this article troubles its narrative of progress. In particular, we investigate the terms on which recent legal struggles have advanced, as well as the ways in which resistance to the legal recognition has been expressed and dealt with. We argue that to the extent that feminist critiques of marriage, familial ideology, and the privatisation of economic responsibility are marginalised, conservative and heteronormative discourses on marriage and family are reinforced. Our case studies include two pivotal moments in the quest for legislative recognition of same-sex relationships: the Hearings of the Canadian House of Commons Standing Committee on Justice and Human Rights on Bill C-23, The Modernization of Benefits and Obligations Act, in 2000 and the hearings on Same-Sex Marriage in 2003. We find that the debates operated within a narrow paradigm that bolstered many existing hierarchies and exacerbated conditions for those who are economically disadvantaged. 相似文献
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280.
Claire Annesley Isabelle Engeli Francesca Gains 《European Journal of Political Research》2015,54(3):525-542
This article investigates the factors that drive governments to pay attention to gender equality issues and place them upon executive agendas. In line with studies of the dynamics of issue attention, which demonstrate the importance of investigating variability in the attention policy makers give to issue demands across policy domains, this article argues that policy issues related to gender equality are multidimensional and patterns in executive attention vary across the different types of gender issues. Multidimensionality of gender equality issues reflects different dynamics in agenda‐setting as different issues invoke contrasting constellations of political representation, institutional friction and veto points. To investigate this variation, this article proposes a twofold distinction between class‐based and status‐based gender equality issues and assesses the validity of three sets of explanations for when gender issues succeed in reaching executive agendas: women in politics, party ideology and economic performance. Drawing on governmental attention datasets from the Comparative Agendas Project, a systematic comparative quantitative analysis of the determinants of gender equality issue attention in five Western European countries is conducted. The main findings confirm that the mechanisms through which different types of gender equality issues gain executive attention differ according to the kind of the gender equality demand. Costly class‐based gender equality issues are more likely to receive executive attention when the economy is performing well, when there is a strong presence of Social Democrats and when there is a high proportion of female MPs. In contrast, economic performance, party politics and women's parliamentary presence do not seem to exert any impact on status‐based issues. Instead, critical actors in the government seem to be the strongest driver for attention over this second type of gender equality issue. This study contributes a gendered dimension to the policy agendas scholarship, adding theoretical and empirical depth to the understanding of how non‐core issues secure their place on full governmental agendas. By focusing on how to secure governmental attention for gender equality issues, the article makes a major contribution to understanding the initial genesis of gender equality policies. 相似文献