Forensic comparison of soils by bacterial community DNA profiling

This preliminary investigation has shown that a soil microbial community DNA profile can be obtained from the small sample of soil recovered from the sole of a shoe, and from soil stains on clothing. We have also shown that these profiles are representative of the site of collection and therefore could potentially be used as associative evidence to prove a link between suspects and crime scenes. Soil community profiles were obtained using the T-RFLP fingerprinting method that uses fluorescent primer technology and semi-automated analysis techniques similar to those used in human DNA profiling in forensic laboratories.     

Comparison of GC-MS and EIA results for the analysis of methadone in oral fluid

The purpose of these studies was to evaluate the performance characteristics of the Cozart Microplate Enzyme Immunoassay (EIA) for the determination of methadone in oral fluid from patients in a drug misuse treatment program. Oral fluid specimens were collected using the Cozart RapiScan Collection system from 198 donors who were receiving treatment for their addiction and were monitored for drug misuse. Oral fluid specimens were also collected from forty volunteer donors who were not drug users. The specimens were analyzed in the laboratory by EIA and then analysed for methadone and its main metabolite EDDP by gas chromatography-mass spectrometry (GC-MS). A total of 103 samples were confirmed positive for methadone. The Cozart Microplate EIA for d-Methadone in oral fluid using a cutoff of 30 ng/mL in diluted oral fluid had a sensitivity of 91.3% +/- 2.8% and a specificity of 100% +/- 1.0% vs. GC-MS.     

Anthropological measurement of lower limb and foot bones using multi-detector computed tomography

Anthropological examination of defleshed bones is the gold standard for osteological measurement in forensic practice. However, multi-detector computed tomography (MDCT) offers the opportunity of three-dimensional imaging of skeletal elements, allowing measurement of bones in any plane without defleshing. We present our experiences of the examination of 15 human lower limbs in different states of decomposition using MDCT. We present our method of imaging and radiological measurement of the bones including sex assessment. The radiological measurements were undertaken by three professional groups--anthropology, radiology, and forensic pathology--both at the site of scanning and at a remote site. The results were compared to anthropological oestological assessment of the defleshed bones. We discuss the limitations of this technique and the potential applications of our observations. We introduce the concept of remote radiological anthropological measurement of bones, so-called tele-anthro-radiology and the role that this could play in providing the facility for standardization of protocols, international peer review and quality assurance schemes.     

Do street traders have the ‘right to the city’? The politics of street trader organisations in inner city Johannesburg,post-Operation Clean Sweep

Street trader organisations are paradoxical objects of study. Their claims resist being analysed through the ‘right to the city’ lens, so contested are rights to inner city spaces between multiple users, not all of them in dominant socioeconomic positions; and so ambiguous is the figure of the street trader, oppressed but also appropriating public space for profit, increasingly claiming, in neoliberalising cities, an entrepreneurial identity. In the aftermath of the 2013 ‘Operation Clean Sweep’ (in which the City of Johannesburg unsuccessfully attempted to evict street traders from its inner city), this paper unpacks the politics of street trader organisations: how they organise their constituencies, frame their claims, forge unlikely alliances and enter into disempowering conflicts in engagements with a divisive municipality.     

Self-determination: The Republican Feminist Agenda

This article defines a republican feminist agenda by reference to the key term ‘self-determination’, in its nationalist and its feminist meanings. It describes the activities of a republican feminist group, Clár na mBan, including a conference organized in March 1994, and initiatives taken since then.