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11.
Bill M Gill P Curran J Clayton T Pinchin R Healy M Buckleton J 《Forensic science international》2005,148(2-3):181-189
Several years ago, a theory to interpret mixed DNA profiles was proposed that included a consideration of peak area using the method of least squares. This method of mixture interpretation has not been widely adopted because of the complexity of the associated calculations. Most reporting officers (RO) employ an experience and judgement based approach to the interpretation of mixed DNA profiles. Here we present an approach that has formalised the thinking behind this experience and judgement. This has been written into a computer program package called PENDULUM. The program uses a least squares method to estimate the pre-amplification mixture proportion for two potential contributors. It then calculates the heterozygous balance for all of the potential sets of genotypes. A list of "possible" genotypes is generated using a set of heuristic rules. External to the programme the candidate genotypes may then be used to formulate likelihood ratios (LR) that are based on alternative casework propositions. The system does not represent a black box approach; rather it has been integrated into the method currently used by the reporting officers at the Forensic Science Service (FSS). The time saved in automating routine calculations associated with mixtures analysis is significant. In addition, the computer program assists in unifying reporting processes, thereby improving the consistency of reporting. 相似文献
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Since 1995 the Forensic Science Service (FSS) has carried out DNA profiling of reference samples for the UK National DNA Database and in forensic casework using two multiplex STR profiling systems. During this period, profiles with anomalous banding patterns, although comparatively rare, have been encountered regularly. The FSS has collected instances of triallelic patterns and aberrant diallelic patterns. A systematic examination of these patterns has provided insight into their underlying genetic cause. The triallelic patterns could be classified into two types based on the relative intensities of their component alleles. In the Type 1 pattern the alleles were of uneven intensity, whereas in the Type 2 pattern, all three alleles were of even intensity. Evidence is presented that the more frequent Type 1 pattern is the result of somatic mutation at a heterozygous locus, and the Type 2 pattern is the result of a localized chromosomal rearrangement at a heterozygous locus. Directly from the Type 1 pattern, it was possible to deduce the size difference between the progenitor and mutated allele. All mutational changes were found to be multiples of four nucleotides, suggesting the loss or addition of one or more tetrameric repeat units. Aberrant diallelic patterns were identified by analysts due to an unexpectedly large difference in intensity between alleles at a heterozygous locus. While some of these diallelic patterns are likely caused by the same genetic phenomena described above occurring at a homozygous locus, others are demonstrated to be caused by a mutation in the primer binding sequence, leading to a reduction in amplification efficiency of one allele. It is concluded that based on a visual inspection of a profile, it is possible to infer a likely genetic basis directly from the triallelic pattern. By contrast, the aberrant diallelic patterns can be due to any one of a number of possible genetic effects. 相似文献
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Through a discussion of the work of Marie Cardinal and Annie Ernaux, this article aims to problematize the anglophone academic world's tendency to associate French feminisms predominantly with avant-garde or highly theoretical texts. The work of Ernaux and Cardinal is presented alongside a discussion of its reception by readers and critics in France, and by academics in English-speaking countries. The first part of the article identifies aspects of Ernaux's and Cardinal's works which cannot be encompassed within a critical framework based on the dichotomy between naive realism on the one hand and the politically and linguistically radical text on the other. Ernaux's plain language, for instance, is clearly very unlike the linguistic experimentation of ‘feminine writing’ nonetheless the emphasis on social class in her writing constitutes a political intervention which is at least equally valid.The reception study in the second part of the article provides further evidence of the relevance to gender politics in France of Cardinal's The Words to Say It (1975) and texts published by Annie Ernaux in the 1980s and 1990s. The ambivalent response of critics seems to indicate the troubling nature of writing which combines the codes of realism and autobiography (or autobiographical fiction in Cardinal's case) with the depiction of taboo subjects such as menstruation, or a daughter's response to her mother's debilitating illness and death. The article also charts the widespread popularity of these texts in France, particularly with women readers, and gives some examples of the pleasures described in letters to the authors. In conclusion, we argue that the ambivalent space between popular and high culture occupied (albeit differently) by Ernaux and Cardinal may be particularly effective in terms of gender politics, and that even in the late 1990s, the personal may be as political as ever. 相似文献
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Managing incidental findings in human subjects research: analysis and recommendations. 总被引:8,自引:8,他引:0
Susan M Wolf Frances P Lawrenz Charles A Nelson Jeffrey P Kahn Mildred K Cho Ellen Wright Clayton Joel G Fletcher Michael K Georgieff Dale Hammerschmidt Kathy Hudson Judy Illes Vivek Kapur Moira A Keane Barbara A Koenig Bonnie S Leroy Elizabeth G McFarland Jordan Paradise Lisa S Parker Sharon F Terry Brian Van Ness Benjamin S Wilfond 《The Journal of law, medicine & ethics》2008,36(2):219-48, 211
No consensus yet exists on how to handle incidental findings (IFs) in human subjects research. Yet empirical studies document IFs in a wide range of research studies, where IFs are findings beyond the aims of the study that are of potential health or reproductive importance to the individual research participant. This paper reports recommendations of a two-year project group funded by NIH to study how to manage IFs in genetic and genomic research, as well as imaging research. We conclude that researchers have an obligation to address the possibility of discovering IFs in their protocol and communications with the IRB, and in their consent forms and communications with research participants. Researchers should establish a pathway for handling IFs and communicate that to the IRB and research participants. We recommend a pathway and categorize IFs into those that must be disclosed to research participants, those that may be disclosed, and those that should not be disclosed. 相似文献
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What makes people join a political party is one of the most commonly studied questions in research on party members. Nearly all this research, however, is based on talking to people who have actually joined parties. This article simultaneously analyses surveys of members of political parties in Britain and surveys of non-member supporters of those same parties. This uniquely enables us to model the decision to join parties. The results suggest that most of the elements that constitute the influential ‘General Incentives Model’ are significant. But it also reveals that, while party supporters imagine that selective benefits, social norms and opposing rival parties’ policies are key factors in members’ decisions to join a party, those who actually do so are more likely to say they are motivated by attachments to their party’s values, policies and leaders, as well as by an altruistic desire to support democracy more generally. 相似文献
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Angela Mashford-Pringle Denise Webb 《Canadian public administration. Administration publique du Canada》2023,66(1):62-77
Aiming to improve the health of First Nations in 1979, the federal government implemented the Indian Health Policy to strengthen community development and the relationship with First Nations peoples, and nurture a trilateral relationship in the healthcare landscape. The Indian Health Policy remains the foundation for First Nations health policy and program development today, even without ever having an implementation plan. We critically appraise the Indian Health Policy to identify gaps and evaluate its impact and progress on subsequent policy evaluations, developments, and recent events in light of the new distinctions-based Indigenous health legislation underway. Based on our findings, we provide recommendations to inform the federal government's efforts to co-develop distinctions-based Indigenous health legislation. 相似文献
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