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We describe a novel method for rapidly identifying and distinguishing between different DNA sequences using short tandem repeat (STR) analysis and DNA microarrays. The method can be used to deduce identity, length, and number of STRs of the target molecule. We refer to this technique as the "variable-length probe array" method for STR profiling (VLPA). The method involves hybridization of the unknown STR target sequence to a DNA microarray displaying complementary probes that vary in length to cover the range of possible STRs. A post-hybridization enzymatic digestion of the DNA hybrids is then used to selectively remove labeled single-stranded regions of DNA from the microarray surface. The number of repeats in the unknown target is then deduced based on the pattern of target DNA that remains hybridized to the array. This DNA profiling technique is useful for performing forensic analysis to uniquely identify individual humans or other species. 相似文献
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Allan, Loughlin and Walker represent leading theorists within the realm of public law analysis. Accordingly, when such theorists write on a similar topic, such as that of the theory of constitutionalism, it can be assumed that their analysis and evaluation of the theory represents a ‘realistic’ account. However, close examination of their writings does not reveal similarity but instead much divergence, even incompatibility. This then raises the question, how can such diversity represent reality? If all three theorists are examining the same phenomenon then surely there must be some similarity between their accounts for there to be reality? Alternatively, if all the perceptions of the theorists are indeed real, then perhaps it is the way that public lawyers represent reality that needs to be examined. 相似文献
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AN INTRODUCTION TO THE LAW OF TRUSTS. By Simon Gardner. [Clarendon Press. Oxford. 1990. First Edition. 250 pp. £25.00 (hardback), £11.95 (paperback).] EXPLAINING CONSTRUCTIVE TRUSTS. By Gbolahan Elias. [Clarendon Press. Oxford. 1990. First Edition. 177 pp. £25.00 (hardback).] CHARLESWORTH &; PERCY ON NEGLIGENCE. By R. A. Percy. [Sweet &; Maxwell. Eighth Edition. 1,232 pp. incl. index. £135.00 (hardback).] CLARITY FOR LAWYERS. By Mark Adler. [The Law Society. 1990. First edition. 128 pp. £10.00 (paperback).] PLANNING LAW AND PROCEDURE. By A. E. Telling. [Butterworths. Eighth edition. 385 pp. incl. index. £16.95 (paperback).] PRACTICAL APPROACH TO PLANNING LAW. By V. Moore. [Blackstone Press Ltd. Second edition. 401 pp. incl. index &; appendices. £19.95 (paperback).] THE EVIDENCE OF CHILDREN. By J. R. Spencer and R. Fun. [Blackstone Press. 1990. 362 pp. £19.50 (paperback).] CROSS ON EVIDENCE. By C. Tapper. [Butterworths. 1990. Seventh edition. 757 pp. £22.95 (paperback).] EMPLOYMENT LAW. By C. J. Carr and P. J. Kay. [M. &; E. Handbook. Pitman Publishing. 1990. Fifth edition, xxiv &; 232 pp. (inc. index). £7.99 (paperback).] REVISION WORKBOOK: LAND LAW. By Gordon Henry. A PARENTS’ GUIDE TO THE LAW. By Jeannie Mackie and Lesley Taylor [Penguin Books. 1990. 419 pp. (inc. index). £7.99 (paperback).] A GUIDE TO INHERITANCE CLAIMS. By Nasreen Pearce. [Fourmat Publishing. 1989. 162 pp. £12.95 (paperback).] 相似文献
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