A novel histological technique for distinguishing between epithelial cells in forensic casework

There are a number of forensic cases in which the identification of the epithelial cell type from which DNA originated would provide important probative evidence. This study aimed to develop a technique using histological staining of fixed cells to distinguish between skin, buccal and vaginal epithelium. First, 11 different stains were screened on formalin-fixed, wax-embedded cells from five women. Samples were analysed qualitatively by examining staining patterns (colour) and morphology (absence or presence of nuclei). Three of the staining methods – Dane's, Csaba's and Ayoub-Shklar – were successful in distinguishing skin epithelial cells from buccal and vaginal. Second, cells were smeared directly onto slides, fixed with one of five fixatives and stained with one of the three stains mentioned above. Methanol fixation, coupled with the Dane's staining method, specific to keratin, was the only technique that distinguished between all three cell types. Skin cells stained magenta, red and orange and lacked nuclei; buccal cells stained predominantly orange–pink with red nuclei; while vaginal cells stained bright orange with orange nuclei and a blue extracellular hue. This staining pattern in vaginal cells was consistent in samples collected from 50 women aged between 18 and 67. Identification of cell type from unlabelled micrographs by 10 trained observers showed a mean success rate of 95%. The results of this study demonstrate that histological staining may provide forensic scientists with a technique for distinguishing between skin, buccal and vaginal epithelial cells and thus would enable more conclusive analyses when investigating sexual assault cases.     

Hurricane Mitch, Alemán, and Other Disasters for Women in Nicaragua

Hurricane Mitch caused unprecedented destruction when it swept through Nicaragua and Honduras in October 1998. Official reports estimated that 3,332 were reported killed or missing and an estimated $1.5 billion damages were caused in Nicaragua alone. Though Mitch did not cause Nicaragua's impoverishment, the severity of its destruction revealed a deeper crisis that has been troubling Nicaragua's economy for over a decade—an economic crisis aggravated by heavy external debts and structural adjustment policies. Using feminist analysis, the case of Nicaragua is explored to consider the effects of natural disasters from a global perspective and to rethink the long-term merits of structural adjustment and foreign aid.     

Jealousy,intimate abusiveness,and intrusiveness

An examination of self-report scales of 160 men and 76 of their partners or former partners found significant correlations between jealousy and abusiveness (for coupled dyads) or intrusiveness (for separated dyads). Jealousy was related to borderline personality and to MCMI-II measures of Post Traumatic Stress Disorder. Rejection sensitivity leading to pathological acts, such as abusiveness and intrusiveness, is seen as originating in early insecure attachment and exposure to shaming experiences.     

“Legitimate Strengths in Criminal Networks”

Extending from the organizing crime perspective, we study how legitimate world actors contribute to structuring a criminal network. This focus also underscores the facilitating role that some participants have in criminal settings. Based on a case study of an illegal drug importation network that was monitored by law-enforcement investigators over a two-year period, we find that although most legitimate actors (or non-traffickers) had nominal roles and contributed minimally to the criminal network's structure, a minority of these actors were critical to the network in two ways: (1) they were active in bringing other participants (including traffickers) into the network; and (2) they were influential directors of relationships with both non-traffickers and traffickers. The presence of such influential participants from legitimate occupational settings illustrates how upperworld figures can facilitate criminal enterprise beyond the mere contributions of legitimate status and expertise.