STR analysis of artificially degraded DNA-results of a collaborative European exercise

Degradation of human DNA extracted from forensic stains is, in most cases, the result of a natural process due to the exposure of the stain samples to the environment. Experiences with degraded DNA from casework samples show that every sample may exhibit different properties in this respect, and that it is difficult to systematically assess the performance of routinely used typing systems for the analysis of degraded DNA samples. Using a batch of artificially degraded DNA with an average fragment size of approx. 200 bp a collaborative exercise was carried out among 38 forensic laboratories from 17 European countries. The results were assessed according to correct allele detection, peak height and balance as well as the occurrence of artefacts. A number of common problems were identified based on these results such as strong peak imbalance in heterozygous genotypes for the larger short tandem repeat (STR) fragments after increased PCR cycle numbers, artefact signals and allelic drop-out. Based on the observations, strategies are discussed to overcome these problems. The strategies include careful balancing of the amount of template DNA and the PCR cycle numbers, the reaction volume and the amount of Taq polymerase. Furthermore, a careful evaluation of the results of the fragment analysis and of automated allele calling is necessary to identify the correct alleles and avoid artefacts.     

„Spielarten des Kapitalismus“ und institutionelle Komplementaritäten in der Makroökonomie — Eine empirische Analyse

Der Beitrag geht der Frage nach, wie national unterschiedliche politökonomische Institutionen die Leistungskraft entwickelter Marktwirtschaften beeinflussen und ob institutionelle Komplementaritäten in der Makroökonomie existieren. Der Ansatz der ?Spielarten des Kapitalismus“ behauptet, dass es diese Komplementaritäten gibt und sich die Länder entsprechend dem in den ökonomischen Sphären vorherrschenden Modus der Koordination in klare Gruppen unterscheiden lassen. In dem Artikel werden die Kernaussagen des Ansatzes einer Reihe von empirischen Tests unterzogen, die auf einer zusammenfassenden Analyse einer Reihe von Fallbeispielen beruht, Die empirischen Ergebnisse bestätigen die Annahmen in überzeugender Weise. Dieliberalen undkoordinierten Marktwirtschaften variieren systematisch entsprechend dem relativen Verhältnis zwischen marktförmiger und strategischer Koordination. Institutionelle Komplementaritäten finden sich in beiden Typen, und sie bringen gesamtwirtschaftliche Leistungszuwächse hervor. Abschließend werden die Muster des institutionellen Wandels in den entwickelten politischen Ökonomien analysiert und einige politische Implikationen der Befunde diskutiert.     

Toward an Empirical Approach to Evidentiary Ruling

This paper responds to criticisms/misconstruals of our measure of the maximum probative value of evidence (D. Davis & W. C. Follette, 2002), and our conclusions regarding the potentially prejudicial role of intuitive profiling evidence, including motive. We argue that R. D. Friedman and R. C. Park's (2003) criticisms and example cases are largely based on inappropriate violation of the presumption of innocence. Further, we address the merits of our absolute difference measure of probative value versus those of the Bayesian likelihood ratio championed by D. H. Kaye and J. J. Koehler (2003). We recommend methods for presentation of measures of evidence utility that convey complexities of interdependence between new and existing evidence. Finally, we propose a probable cause standard for admission of potentially prejudicial evidence, dictating that admissibility of such evidence should be contingent upon other substantial evidence of guilt.     

Polyclonal systemic immunoblast proliferation: an unusual hematologic entity presenting as a medical examiner case

A 43-year-old woman who was receiving oral antibiotics for several days for a superficial foot infection developed a persistent rash, fever, and lymphadenopathy, despite discontinuation of the antibiotic and administration of steroids for a presumed drug reaction. Hours after a subsequent visit to the emergency room for worsening symptoms, she died at home. At autopsy, there was a florid, systemic proliferation of polyclonal plasma cells and immunoblasts infiltrating nearly every organ and tissue of the body, most notably the lymph nodes and spleen. The polyclonal nature of the process was confirmed by immunofixation electrophoresis and immunohistochemistry. Cases of fatal polyclonal systemic immunoblast proliferations are extremely rare, and the trigger for such proliferations is not always known. We review the literature on this unusual entity and discuss the clinical and pathologic findings.     

Authors Index Volume 12

Authors Index

Authors Index Volume 12     

Microscopic characteristics of hacking trauma

The purpose of this study was to determine if it is possible to associate machetes, axes, and cleavers with the microscopic parallel striations they leave on the cut surfaces of the bone. Hacking trauma was experimentally inflicted on pig bones using machetes, axes, and cleavers. Negative impressions of both the cut surfaces of the bone and the weapon blades were analyzed using scanning electron microscopy. The results of this investigation indicate that it is possible to correlate a class of hacking weapons to trauma inflicted on bone by these weapons.     

The Evolving Structure of the Environmental Justice Movement in the United States: New Models for Democratic Decision-Making

Over the last two decades in the United States, mainstream environmental organizations have reduced, rather than increased, democratic participation by citizens in environmental problem-solving. The environmental justice movement, on the other hand, has served to enlarge the constituency of the environmental movement by incorporating poorer communities and oppressed people of color into environmental decision making process; build community capacity by developing campaigns and projects that address the common links between various social and environmental problems; and facilitate community empowerment by emphasizing grassroots organizing over advocacy. This paper outlines the different components in the environmental justice movement. It is our contention that if researchers and policymakers continue to conceive of the ecological crisis as a collection of unrelated problems, then it is possible that some combination of regulations, incentives, and technical innovations can keep pollution and resource destruction at tolerable levels for more affluent socioeconomic populations. However, poor working class communities and people of color which lack the political–economic resources to defend themselves will continue to suffer the worst abuses. However, if the interdependency of issues is emphasized as advocated by the environmental justice movement, then a transformative environmental politics can be invented.     

A comparative evaluation of a new generation jail

This study reports findings from an evaluation of a new generation jail in a large southwestern state. The jail is one component of a complex containing a traditional jail, an indirect supervision facility (barracks), and the new generation jail. Using survey and operational data, we compare the new generation jail to the other two facilities. The findings provide generally positive support for the effectiveness of the new generation jail. Inmates and staff were much more satisfied with the physical facilities. Staff perceived it as more secure, though they reported only limited advantages in safety and security. Violence and disciplinary problems were substantially lower. However, no savings in staffing levels were noted, nor were there differences in job satisfaction for staff in the new generation jail. We discuss the implications of the findings and suggest additional directions for jail evaluations.     

The relation between the ethics of personal conscience—social responsibility and principled moral reasoning

Three samples of college students were tested on Hogan's Survey of Ethical Attitudes (SEA) and Rest's Defining Issues Test (DIT), a test of moral reasoning ability in the Kohlbergian tradition. In addition, one of the samples took Collins's revision of Rotter's Internal-External Scale (I-E) while another sample took Snyder's Self-Monitoring Scale. There was a small but reliable tendency for subjects who endorsed the ethics of personal conscience, as measured by the SEA, to show greater maturity in moral reasoning, as assessed by the DIT. This result was seen as raising some interesting questions about the relation between liberalism-conservatism and moral maturity. The subjects who advocated the ethics of social responsibility tended to show more internal locus of control as measured by the I-E scale. There also was a tendency for subjects who preferred the morality of conventional role-conformity, as measured by the DIT, to have high scores on the Self-Monitoring Scale. The correlations between personal conscience and mature moral reasoning, while significant, were small enough to make it seem that people of various social and political attitudes are likely to achieve maturity in moral reasoning.Received his Ph.D. from Michigan State University in 1973. Current research interests include moral judgment, family interaction, and alcoholism.Received his Ph.D. from Temple University in 1972. Research interests include attribution theory, social comparison theory, moral judgment, and the relation between personality and cardiac health.