Interpretation of a 3,4-methylenedioxymethamphetamine (MDMA) blood level: discussion by means of a distribution study in two fatalities

The amphetamine derivative 3,4-methylenedioxymethamphetamine (MDMA, "Ecstasy" is a currently used or abused designer drug and fatalities are frequently encountered in forensic practice. However, the question remains open whether an MDMA blood level can be toxic or even potentially lethal. In order to provide insight in the interpretation of a detected MDMA concentration, the distribution of MDMA and its metabolite 3,4-methylenedioxyamphetamine (MDA) in various body fluids and tissues was studied and discussed in two different fatalities. Apart from peripheral blood samples (such as femoral and subclavian blood), various blood samples obtained centrally in the human body and several body fluids (such as vitreous humour) were examined. In addition, various tissues such as cardiac muscle, lungs, liver, kidneys, and brain lobes were analysed. In contrast to the peripheral blood levels, high MDMA and MDA levels were found in cardiac blood and the majority of the organs, except for the abdominal adipose tissue. The high concentrations observed in all lung lobes, the liver and stomach contents indicate that post-mortem redistribution of MDMA and MDA into cardiac blood can occur and, as a result, blood sampled centrally in the body should be avoided. Therefore, our data confirm that peripheral blood sampling remains "the golden standard". In addition, a distinct difference in peripheral blood MDMA concentrations in our two overdose cases was established (namely 0.271 and 13.508 microg/ml, respectively). Furthermore, our results suggest that, if a peripheral blood sample is not available and when putrefaction is not too pronounced, vitreous humour and iliopsoas muscle can be valuable specimens for toxicological analysis. Finally, referring to the various mechanisms of death following amphetamine intake, which can result in different survival times (e.g. cardiopulmonary complications versus hyperthermia), the anatomo-pathological findings and the toxicological results should be considered as a whole in arriving at a conclusion.     

Suicide with inert gases: addendum to Final Exit

Suicide by asphyxiation with a plastic bag placed over the head is a method that has been advocated by right-to-die groups. Recently, such groups have proposed the introduction of helium into the plastic bag as a means of hastening death. Helium is readily available at toy stores, where it is sold in tanks for balloon inflation. It produces asphyxiation by the exclusion of oxygen in enclosed spaces. We report 7 fatalities throughout an 18-month period involving plastic bag suffocation in conjunction with helium use. These fatalities coincide with publication of an update to a popular right-to-die text in which this method is described. Although right-to-die literature was absent from all scenes, this method was not previously observed in our jurisdiction, and the deaths likely reflect exposure to this information. Because of analytical difficulties in testing for helium in biologic specimens, death certification rests on scene investigation.     

Population genetic study of eight short tandem repeat loci CSF1PO,TPOX, TH01, F13A01, FESFPS,vWA, F13B and LPL in the Western Romanian population

Allele frequencies for eight tetranucleotide short tandem repeat (STR) loci-CSF1PO, TPOX, TH01, F13A01, FESFPS, vWA, F13B, LPL-were obtained from a population sample of 105-122 unrelated individuals born in Transylvania and Banat (Romania).     

Taphonomic mycota: fungi with forensic potential

Forensic archaeologists and criminal investigators employ many different techniques for the location, recovery, and analysis of clandestine graves. Many of these techniques are based upon the premise that a grave is an anomaly and therefore differs physically, biologically, or chemically from its surroundings. The work reviewed in this communication demonstrates how and why field mycology might provide a further tool towards the investigation of scenes of crime concealed in forest ecosystems. The fruiting structures of certain fungi, the ammonia and the postputrefaction fungi, have been recorded repeatedly in association with decomposed mammalian cadavers in disparate regions of the world. The ecology and physiology of these fungi are reviewed briefly with a view to their potential as a forensic tool. This application of mycology is at an interface with forensic archaeology and forensic taphonomy and may provide a means to detect graves and has the potential to estimate postburial interval.     

An alternative method to screen for pepper spray residue

A method was developed to screen for pepper spray residue using instruments and methods other than those techniques commonly employed to analyze chemical residue (i.e.. gas chromatography mass spectrometry-GCMS or liquid chromatography mass spectrometry-LCMS). The method employed gas chromatography (GC), thin layer chromatography (TLC), and diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) to screen for dried pepper spray stains. Pepper sprays from nine different manufacturers were investigated. Capsaicin and dihydrocapsaicin were identified and unique IR reflectance spectra are presented. An additional five compounds were presumptively found. Results showed that a particular stain could be characterized as a pepper-based stain.     

Further studies on spot tests and microcrystal tests for identification of cocaine

The presence of cocaine in illicit drug samples is still being determined in some laboratories using spot tests and microcrystal tests. Seventeen chemical species were tested using three different spot tests (Wagner, Marquis, and cobalt thiocyanate followed by stannous chloride reactions) and two microcrystal tests (gold chloride and platinic chloride) to determine whether the results could be differentiated from the results of these tests on cocaine. The data obtained indicated that nine of the 17 compounds gave results similar to those from cocaine using the three spot tests, but that the results from microcrystal testing allowed for differentiation of all nine compounds from cocaine.     

Forensic medical assessment of the skull bone fractures owing to blow impacts depending on anatomical characteristics and a nature of a trauma-causing objects

Fractures in bones of the skull base (BSB) are preconditioned by the contact zone of interaction between the two below surfaces, i.e. the trace-forming one (object) and the trace-receiving one (bone). On the basis of their independent research, the authors suggest a set of criteria for the expert evaluation of fractures in BSB for the purpose of specifying the properties of the trauma-causing object, which is made with due regard for the anatomic specific features of the trauma zone.     

The ordinal localization of multiple mono-type carpal bones

The case study was based on the osteologic collection of the chair for anthropology, State Moscow University, including the male carpal bones (67) and female carpal bones (13), aged above 18 to 20. Four measurements were made according to Martin's scheme for metacarpal bones and finger phalanxes. A diagnostic method was worked out on the bases of the discriminative analysis, with such method enabling the remodeling of the ordinal localization of metacarpal bones as well as of main, medium and distal phalanxes. The maximally accurate classification was ensured for metacarpal bones, ranging from 81.5% to 100%; and the minimally accurate one was achieved for distal phalanxes, ranging from 20% to 95%. The discriminative analysis results were checked for distal phalanxes by Neklyudov's series (male--100, female--85); they were described according to 7 sizes. However, an attempt to enhance the accuracy of the classification of these phalanxes failed. The offered method should be applied in combination with the traditional anatomic-and-morphological method; besides, if possible, it is necessary to check the congruence of joint surfaces in the metacarpal interphalangeal joints.