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191.
McNevin D Wilson-Wilde L Robertson J Kyd J Lennard C 《Forensic science international》2005,153(2-3):247-259
A feasibility study of short tandem repeat (STR) genotyping of telogen phase hairs in particular, and hair shaft in general, is presented. A number of extraction procedures in common use were investigated and the quantities of nuclear DNA (nuDNA) delivered were quantified via a real-time PCR assay. The extracts were subjected to two variations on AmpFlSTR Profiler Plus PCR amplification strategies (extended cycles, two rounds of PCR) and the genotypes compared. Nuclear DNA was found to persist in human hair shafts, albeit at very low levels. Full Profiler Plus profiles consistent with the hair donor were obtained from 100 mg hair shaft samples (bleached and unbleached). These were, however, mixed profiles, indicating low copy number (LCN) contamination in the extracts. Single telogen hair clubs and single hair shafts delivered partial profiles with usually only one allele of heterozygous loci. Telogen phase hairs yielded the same amount of nuDNA (and no more) as hair shafts (either anagen or telogen). Whether hair shafts dissolved or not in lysis buffer had no effect on either the quantitated yield of DNA or on the chance of obtaining a correct genotype. These results provide evidence that genomic DNA resides on the exterior of the hair shaft and we use this information to suggest an optimal procedure for nuDNA extraction from keratinised hair samples: soaking hairs in simple digestion buffers containing Tris-HCl, a salt and a chelating agent without prior cleaning of the hair shafts. It is proposed that cleaning removes most of the recoverable DNA. This procedure was applied to obtain genotypes from 3 cm hair shafts which matched reference profiles from the donors at up to 9 out of 10 AmpFlSTR Profiler Plus STR loci. When the genotyping success was measured by counting the number of matches between the two dominant alleles at each locus for each extract with the reference DNA profile of the hair donor, the success was found to be highly dependent on the donor. The number of matching alleles varied between not less than 10 for one donor to no more than two for another donor. These results may well be linked to the environmental experience of the hairs from each donor prior to removal. 相似文献
192.
Mulero JJ Chang CW Lagacé RE Wang DY Bas JL McMahon TP Hennessy LK 《Journal of forensic sciences》2008,53(4):838-852
DNA typing of degraded DNA samples can be a challenging task when using the current commercially available multiplex short tandem repeat (STR) analysis kits. However, the ability to type degraded DNA specimens improves by redesigning current STR marker amplicons such that smaller sized polymerase chain reaction (PCR) products are generated. In an effort to increase the amount of information derived from these types of DNA samples, the AmpFlSTR MiniFiler PCR Amplification Kit has been developed. The kit contains reagents for the amplification of eight miniSTRs which are the largest sized loci in the AmpFlSTR Identifiler PCR Amplification Kit (D7S820, D13S317, D16S539, D21S11, D2S1338, D18S51, CSF1PO, and FGA). Five of these STR loci (D16S539, D21S11, D2S1338, D18S51, and FGA) also are some of the largest loci in the AmpFlSTR SGM Plus kit. This informative nine-locus multiplex, which includes the gender-identification locus Amelogenin, has been validated according to the FBI/National Standards and SWGDAM guidelines. Our results demonstrate significant performance improvements in models of DNA degradation, PCR inhibition, and nonprobative samples when compared to the AmpFlSTR Identifiler and SGM Plus kits. These data support that the MiniFiler kit will increase the likelihood of obtaining additional STR information from forensic samples in situations in which standard STR chemistries fail to produce complete profiles. 相似文献
193.
194.
Dennis Kurzon 《International Journal for the Semiotics of Law》2011,24(2):195-209
The paper looks at the establishment of religion clause in the First Amendment to the US Constitution, and cases, e.g. Brown v. Gilmore, followed by Croft v. Perry and Sherman v. Koch, cases that relate to the concept of the “moment of silence” in educational institutions in which it was claimed that such
events constitute a breach of the establishment clause. Courts have been inconsistent in their decision-making, which may
indicate a lack of transparency not only in the interpretation of the relevant phrase in the Constitution but also in the
judicial interpretation of the “three-pronged test” with regard to “excessive entanglement” as laid out in Lemon v. Kurtzman of 1971. The paper discusses the “moment of silence” within the framework of a model of silence in which this type of silence
would be labeled as either textual or situational silence. 相似文献
195.
Modeling Bone Surface Morphology: A Fully Quantitative Method for Age‐at‐Death Estimation Using the Pubic Symphysis
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Dennis E. Slice Ph.D. Bridget F. B. Algee‐Hewitt Ph.D. 《Journal of forensic sciences》2015,60(4):835-843
The pubic symphysis is widely used in age estimation for the adult skeleton. Standard practice requires the visual comparison of surface morphology against criteria representing predefined phases and the estimation of case‐specific age from an age range associated with the chosen phase. Known problems of method and observer error necessitate alternative tools to quantify age‐related change in pubic morphology. This paper presents an objective, fully quantitative method for estimating age‐at‐death from the skeleton, which exploits a variance‐based score of surface complexity computed from vertices obtained from a scanner sampling the pubic symphysis. For laser scans from 41 modern American male skeletons, this method produces results that are significantly associated with known age‐at‐death (RMSE = 17.15 years). Chronological age is predicted, therefore, equally well, if not, better, with this robust, objective, and fully quantitative method than with prevailing phase‐aging systems. This method contributes to forensic casework by responding to medico‐legal expectations for evidence standards. 相似文献
196.
A behavioral power index 总被引:1,自引:0,他引:1
We propose an empirically informed measure of the voting power that relaxes the assumptions of equally probable and independent votes. The behavioral power index measures the voter’s ability to swing a decision based on the probability distributions of the others’ behavior. We apply it to the Supreme Court of the United States using roll-call data to estimate voting probability distributions, which lead us to refute the assumption of equally probable and independent votes, and estimate the equivalent number of independent Justices for the Warren, Burger and Rehnquist benches, which turns out to be very low. 相似文献
197.
198.
Dennis Vicencio Blanco 《国际公共行政管理杂志》2016,39(8):620-634
The article revisits the gun control debate policy comparing the varied and diverse perspectives and viewpoints held by both the culturalist and empiricist, pro-gun society and gun less society on how the gun debate may be properly resolved through a pragmatic approach of policy-making- putting emphasis on culture, experience and observation with regards to gun control. In the process of employing an idea-based coalition approach and argumentative discourse analysis towards the brewing gun control debate, the article consequently realizes the convergent role of both cultural worldviews and public opinion plays at the very heart of deliberation on gun debate. 相似文献
199.
Dennis J. Palumbo 《政策研究评论》1986,5(3):598-605
Because corrections is a multibillion dollar growth industry it is attractive to profitmaking firms. The issue is not whether private firms should b e involved in corrections, for they already are; the issue i s how much Involvement should there b e and in what areas. The principal argument used in favor of private involvement is that they will be more efficient than public agencies. But, while this may well be true, the problem is, what impact will this have on corrections policy? Will it increase the move toward retribution and away from rehabilitation? Existing private involvement in corrections has been primarily in the direction of finding workable alternatives to prisons. Private profitmaking firms may take us in the opposite direction. A number of other issues and problems of privatization also are discussed. 相似文献
200.