TWGDAM validation of a nine-locus and a four-locus fluorescent STR multiplex system

The Gene Print PowerPlex 1.1/Amelogenin and FFFL Fluorescent STR Systems have been validated following the recommendations presented by the Technical Working Group on DNA Analysis Methods (TWGDAM). The PowerPlex 1.1/Amelogenin System supports simultaneous amplification of eight short tandem repeat loci and the Amelogenin gender identification marker. The loci D16S539, D7S820, D13S317, and D5S818 are labeled with fluorescein (FL) while the loci CSF1PO, TP0X, TH01, vWA and Amelogenin are labeled with carboxy-tetramethylrhodamine (TMR). The FFFL Multiplex System is composed of the loci F13A01, FESFPS, F13B, and LPL, each labeled with fluorescein. We have observed no overlap of alleles across loci labeled with an individual fluorescent dye. Samples of each system were amplified and labeled in a single reaction, separated by electrophoresis through a denaturing polyacrylamide gel, and amplified alleles detected using a Hitachi FMBIO Fluorescent Scanner. Alterations from the standard amplification protocols in cycle number and annealing temperature generally produced excellent results. In experiments testing sensitivity as little as 0.2 ng of DNA template could be detected. As expected, different body fluids from the same individuals generated identical DNA profile results. Template DNA derived from blood-strains deposited on a variety of matrix supports displayed robust amplification except for material derived from deposits on wood and Japanese orchid leaves. Mixtures of DNA templates could be interpreted with the minor component present in as little as ten percent of the total sample. Monoplex and multiplex amplifications produced identical amplified allele patterns, indicating that STR multiplex systems save template and increase efficiency in the amplification procedure without loss of quality. Analyses of genotype frequencies in African-American, Caucasian-American and Hispanic-American populations using all twelve loci were used to determine matching probabilities smaller than 1 in 1.14 x 10(8) and 1 in 2658 for the PowerPlex 1.1 and the FFFL Multiplex Systems, respectively. The matching probability achieved with the two systems combined is smaller than 1 in 3.03 x 10(11). The independence of alleles within loci was generally demonstrated by applying the exact test to demonstrate Hardy-Weinberg Equilibrium. All of the studies performed indicate that the PowerPlex 1.1/Amelogenin and FFFL Multiplex Systems are powerful, robust, and reliable investigative tools that can be used in the analysis of forensic samples.     

Learning Lessons and Transferring Policy across Time, Space and Disciplines

An area of recent interest in the International Relations and Comparative Politics literature concerns 'policy transfer'. This is a dynamic whereby knowledge about policies, administrative arrangements or institutions is used across time or space in the development of policies, administrative arrangements and institutions elsewhere. Policy transfer is deemed to be on the increase in an era of globalisation. Indeed, some governments and international organisations are proactive in promoting harmonisation and convergence or exporting policy lessons. This paper surveys the state of the burgeoning literature, identifies some methodological issues in studying policy transfer, and outlines some additional routes of research.     

Predictors of Attrition in a Treatment Program for Battering Men

This study examines factors associated with the high attrition rate in treatment programs for men who batter. In accord with past research, we expected demographic variables of age, race, employment status, relationship status, and socioeconomic status to predict attrition. We also hypothesized that attitudinal and personality variables, as well as contextual/program variables, might account for attrition more parsimoniously. Specifically, we hypothesized that attrition would be predicted by frequency and severity of violence, denial of a problem with violence, rigidity of thinking, low levels of self-disclosure, and higher anxiety and constriction in social situations. In addition, we predicted attrition would relate to dependency, maladaptive personality styles, and expectations regarding group counseling (e.g., whether treatment is perceived as aversive). Finally, we proposed that attrition would relate to whether batterer participation in treatment was self-motivated or the result of external pressures. Participants were 61 men enrolled in a batterer treatment program in a mid-sized city. Analyses of variance and discriminant analyses indicated that program attrition was unrelated to demographic, attitudinal, or personality variables. Only the contextual/program variables of mileage traveled to attend and external monitoring of attendance significantly differentiated treatment rejecters, drop-outs, and treatment continuers. Findings are discussed with regard to intrinsic and extrinsic motivational factors. Future directions for exploration are discussed.     

Equity concern and the political economy of protection in New Zealand

A number of empirical models have found protection to be greatest for industries employing poorly skilled, low paid workers. This has caused some economists, notably Robert Baldwin, to suggest that equity concern by politicians in an alternative to the interest group hypothesis. This paper reports the result of a test on New Zealand data that shows that this equity concern is absent for industries with few employees or firms. Equity concern variables were only important for industries with many employees and firms. This suggests that equity concern is selective and may be reconcilable with self-interest motivations.I am grateful to John Tressler, Richard Harris and an anonymous referee for their helpful comments.     

Prevalence of xylazine in overdose cases: An analysis of Miami-Dade County medical examiner case data

Xylazine sedative, muscle relaxant, and analgesic used in a veterinary setting. Although xylazine was never approved for therapeutic use in humans, it has become popular in the street drug market as a cutting or bulking agent in the fentanyl and heroin supply. Recently, there has been a significant increase in the detection of xylazine in postmortem forensic toxicology casework. Xylazine can be identified during routine toxicology screening utilizing instrumentation such as gas chromatography–mass spectrometry and liquid chromatography–mass spectrometry. Using the Miami-Dade Medical Examiner's LIMS system, all cases received between 2015 and 2022 in which xylazine was reported were reviewed. The cases studied include accidental drug overdose deaths in Miami-Dade County as well as Collier County (Naples), Florida. In total, there are 170 cases; the majority are accidental polydrug overdoses involving White males between the ages of 25 and 44 years old. Of the 170 cases, 37% listed xylazine as the cause of death. 13% of cases contained only xylazine and fentanyl while the remaining 87% of deaths were attributed to polydrug toxicity involving two or more substances. The prevalence of xylazine can be attributed to its increasing popularity rather than an increase in caseload. In 2019, xylazine was present in only 4% of all accidental fentanyl overdoses. By 2021, this percentage has increased sixfold, with xylazine present in 24% of all accidental fentanyl overdoses. Despite a decrease in fentanyl overdoses in 2022, the percentage of xylazine detection remained the same.     

Low self-control and coworker delinquency: A research note

Since the publication of Gottfredson and Hirschi [A General Theory of Crime. Stanford, CA: Stanford Univ. Press, 1990], a large amount of research has shown a link between low self-control and delinquency. Some research has revealed that low self-control has not been able to account for the strong effects of peer delinquency on delinquency. Criminological literature has, until recently, neglected the interactional relationship between low self-control and delinquent peers in predicting delinquency. This study used a sample of employed high school seniors to assess the interaction between low self-control and coworker delinquency on occupational delinquency. Regression analyses indicated that the interaction term was a strong predictor of occupational delinquency, even after controlling for several established predictors of delinquency.     

Non-Governmental Policy Transfer: The Strategies of Independent Policy Institutes

Discussions of policy transfer have primarily focused on official actors and networks. The non-governmental mode of policy transfer via foundations, think tanks and non-governmental organizations is a relatively neglected dimension. Accordingly, this paper addresses the role of think tanks in promoting the spread of policy ideas about privatization. The importance of think tanks to policy transfer is their ability to diffuse ideas by (1) acting as a clearing-house for information; (2) their involvement in the advocacy of ideas; (3) their involvement in domestic and transnational policy networks; and (4) their intellectual and scholarly base providing expertise on specialized policy issues. Concepts from comparative public policy and international relations such as "social learning" and "epistemic communities" are used to outline the way think tanks advocate innovative policy ideas. However, not only have think tanks been effective in promoting the transfer of policy ideas, the international spread of think tanks also exhibits organizationaltransfer.