Reliable and Robust Molecular Sexing of the Hen Harrier (Circus cyaneus) Using PCR‐RFLP Analysis of the CHD 1 Gene

The hen harrier (Circus cyaneus) is a bird of prey that is persecuted in the United Kingdom, and there is a need for a DNA‐based individual identification and sexing system for the use in forensic investigations. This study reports a new set of PCR primers for the chromo‐helicase‐DNA‐binding protein 1 gene, which allows sexing using PCR‐RFLP. Instead of exonic primers that amplify across a large intron, this set consists of a primer within the intron, enabling reduction in amplicon sizes from 356 to 212 bp and 565 to 219 bp in W and Z chromosomes. DNA degradation and dilution experiments demonstrate that this set is significantly more robust than one that amplifies across the intron, and sequencing of the intronic primer‐binding region across several individuals shows that it is highly conserved. While our objective is to incorporate this primer set into an STR‐based individualization kit, it may in the meantime prove useful in forensic or conservation studies.     

The Significance of Adrenal Hemorrhage: Undiagnosed Waterhouse‐Friderichsen Syndrome,A Case Series

A retrospective series of five cases of nontraumatic gross adrenal hemorrhage were identified in 800 consecutive forensic autopsies. All patients were males, of different ethnicities and with ages ranging from 2 to 48 years. All patients had a clinical history and autopsy findings suggestive of sepsis. Pre‐ or postmortem microbiological cultures were variably positive for Pseudomonas aeruginosa, Streptococcus pneumoniae, Staphylococcus aureus, and methicillin‐resistant Staphylococcus aureus. The fifth case was positive for yeast and a coagulase negative staphylococcus; contamination of this culture medium cannot be excluded. No cases had a culture positive for Neisseria meningitidis. We find that the reviewed patients with grossly or microscopically identifiable adrenal hemorrhage were otherwise healthy individuals who died suddenly as a consequence of bacterial infection. In each case, signs and symptoms compatible with premortem adrenal insufficiency were reported; in no instance was the adrenal hemorrhage clinically identified.     

Statistical Evaluation of Alternative Light Sources for Bloodstain Photography

Bloodstain photography is important in forensic applications, especially for bloodstain pattern analysis. This study compares the enhancement effect of bloodstain photography using three different types of light source: fluorescent white light, near‐ultraviolet (UV) light‐emitting diode (LED) light, and 410 nm LED light. Randomized complete block designs were implemented to identify the lighting that would statistically produce the best enhancement results for bloodstains on different types of surfaces. Bloodstain samples were prepared on white cotton, brown carpet, tar road, and wood. These samples were photographed in darkroom conditions using a Canon EOS 50D digital SLR camera, with Canon EFS 60 mm f/2.8 Macro USM lens. Two‐way analysis of variance and Fisher's least significant difference test were used to analyze the contrast of the images. The statistical analysis showed that 410 nm light is the best among the tested lights for enhancing bloodstains on the tested surfaces, where the contrast of bloodstain to background was the highest.     

Modified Midi‐ and Mini‐Multiplex PCR Systems for Mitochondrial DNA Control Region Sequence Analysis in Degraded Samples

Two multiplex polymerase chain reaction (PCR) systems (Midiplex and Miniplex) were developed for the amplification of the mitochondrial DNA (mtDNA) control region, and the efficiencies of the multiplexes for amplifying degraded DNA were validated using old skeletal remains. The Midiplex system consisted of two multiplex PCRs to amplify six overlapping amplicons ranging in length from 227 to 267 bp. The Miniplex system consisted of three multiplex PCRs to amplify 10 overlapping short amplicons ranging in length from 142 to 185 bp. Most mtDNA control region sequences of several 60‐year‐old and 400–500‐year‐old skeletal remains were successfully obtained using both PCR systems and consistent with those previously obtained by monoplex amplification. The multiplex system consisting of smaller amplicons is effective for mtDNA sequence analyses of ancient and forensic degraded samples, saving time, cost, and the amount of DNA sample consumed during analysis.     

Characterization of In Vitro Metabolites of CP 47,497, a Synthetic Cannabinoid,in Human Liver Microsomes by LC‐MS/MS

CP 47,497, a potent cannabinoid receptor type 1 agonist, is the main active ingredient in the herbal mixture “Spice” sold in European countries. The illegal use of “Spice” for its psychoactive effects has become a social issue. In this study, the in vitro metabolism of CP 47,497 was investigated in human liver microsomes to characterize the metabolic fate of CP 47,497. CP 47,497 was incubated with human liver microsomes, and the reaction mixture was analyzed using liquid chromatography‐tandem mass spectrometry. A total of eight metabolites were detected in human liver microsomes and structurally characterized based on mass spectral data. The main metabolic pathways involved hydroxylations or oxygenations. The identified metabolites were mono‐oxygenated metabolites (M1 and M4), mono‐hydroxylated metabolites (M3, M5, M6, M7, and M8), and a di‐oxygenated metabolite (M2). The detection of these metabolites could confirm the presence of CP 47,497 in biological samples; therefore, collectively, they would be excellent indicators of “Spice” drug abuse.     

The poor in Singapore: Issues and options

This article examines the changing patterns of poverty in Singapore. As Singapore's population ages, the poor increasingly includes the elderly. It appears that ascribed factors, such as gender and race, have significant influence on financial security at old age. As the population ages, the adequacy of existing anti-poverty policy is challenged.     

Book reviews

Poverty, Progress and Exclusion: an Economic History of Latin America in the Twentieth Century. By Rosemary Thorp. Baltimore, MD: Johns Hopkins University Press for the Inter‐American Development Bank and the European Union, 1998. Pp.xiv + 369. £20.50. ISBN 1 886938 35 0

Economic Dynamism in the Asia‐Pacific: The Growth of Integration and Competitiveness. Edited by Graham Thompson. London: Routledge, 1998. Pp.402. £50 and £14.99. ISBN 0 415 17273 X and 17274 8

India and Pakistan: The First Fifty Years. Edited by Selig S. Harrison, Paul H. Kreisberg, and Dennis Kux. Cambridge: Cambridge University Press, 1999. Pp.xii + 217. £32.50 and £10.95. ISBN 0 521 64185 3 and 64585 9

Agencies in Foreign Aid: Comparing China, Sweden and the United States in Tanzania. Edited by Goran Hyden and Rwekaza Mukandala. New York: St. Martin's Press, 1999. Pp.ix + 246. $69.95. ISBN 0 312 22098 7

Market Integration, Regionalism and the Global Economy. Edited by Richard E. Baldwin, Daniel Cohen, Andre Sapir and Anthony Venables. Cambridge: Cambridge University Press, 1999. Pp. xix + 344. £45 and £16.95. ISBN 0 521 64589 1 and 0 521 64181 0

Instruments of Change: Motivating and Financing Sustainable Development. By Theodore Panayotou. Earthscan in association with the United Nations Environment Programme, 1998. Pp.240. £19.95. ISBN 1 85383 467 X

Famine in Africa: Causes, Responses and Prevention. By Joachim von Braun, Tesfaye Teklu and Patrick Webb. Baltimore, MD and London: The Johns Hopkins University Press, 1999. Pp.xvii + 218. £32.50. ISBN 0 8018 6121 7

The East Asian Welfare Model: Welfare Orientalism and the State. Edited by Roger Goodman, Gordon White and Huck‐ju Kwon. London and New York: Routledge, 1998. Pp.206. £17.99. ISBN 0 415 17211 X

Critical Development Theory: Contributions to a New Paradigm. Edited by Ronaldo Munck and Denis O'Hearn. London and New York: Zed Books, 1999. Pp.xx + 217. £39.95 and £22.50. ISBN 1 85649 637 6 and 638 4

Streetlife China: Transforming Culture, Rights and Markets. By Michael Dutton. Cambridge: Cambridge University Press, 1999. Pp.xvi + 304. £13.95 (US$19.95) and £37.50 (US$54.95). ISBN 0 521 63719 8 and 63141 6     

Analyzing the Multidirectional Relationships Between the President,News Media,and the Public: Who Affects Whom?

In order to study presidential leadership and responsiveness, this research focuses on the role of the news media and examines the multidirectional relationships between the president, the news media, and the public. One of the purposes of this study is to examine competing theoretical expectations about the causal direction between the three actors by focusing on their issue stances. The potentially reciprocal influences between the three actors are estimated by using vector autoregression and moving average representation simulations. According to the statistical results, the news media significantly interact with the public and the president. In contrast, the direct relationship between the president and the public is weak or insignificant.     

Gender and E-Participation in Local Governance: Citizen E-Participation Values and Social Ties

Drawing on motivation theories and social network literature, this research analyzes the relationship between each gender’s perceived intrinsic and instrumental value of participation and the strength of offline social ties and active e-participation in a local governance setting. Based on E-Participation Survey data collected from the members of an e-participation program run by local government in South Korea, the study finds that male participants who perceive greater instrumental value of e-participation are more likely to use e-participation actively. On the other hand, women who perceive a greater intrinsic value of e-participation are more likely to be active users. Female respondents were also more likely to use e-participation when they were connected to offline social groups through weak rather than strong ties, presumably because they use e-participation as a supplementary channel to their social networks. There was no significant relationship between male respondents’ social ties and their active e-participation.     

Promoting Positive Youth Development by Examining the Career and Educational Aspirations of African American Males: Implications for Designing Educational Programs

African American males experience poor academic performance, high absenteeism at school, and are at increased risk of being involved in violence than other racial groups. Given that the educational outlook for African American males appears bleak, it is important to assess the aspirations of these adolescent males in order to find the gap between aspirations and educational attainment. In order to promote positive development within this population, it is essential that factors that affect African American males be identified. A survey was administered to male students attending elementary, middle, and high schools in a local school district. A cross-sectional study was conducted to examine the career and educational aspirations of African American males. A total of 473 males were surveyed: 45% African American, 22% Caucasian, 13% biracial, and 19% Other (including Asian American, Hispanic, Native American). The results revealed that African American males aspired to attend college at the same rate as other ethnic groups. Also, African American males were more likely to aspire to be professional athletes than males from other ethnic groups. Important factors to consider when designing a program are discussed as well as future research and limitations.