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61.
An Optimized DNA Analysis Workflow for the Sampling,Extraction, and Concentration of DNA obtained from Archived Latent Fingerprints
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April D. Solomon M.S. Madison E. Hytinen B.S. Aryn M. McClain M.S. Marilyn T. Miller Ed.D. Tracey Dawson Cruz Ph.D. 《Journal of forensic sciences》2018,63(1):47-57
DNA profiles have been obtained from fingerprints, but there is limited knowledge regarding DNA analysis from archived latent fingerprints—touch DNA “sandwiched” between adhesive and paper. Thus, this study sought to comparatively analyze a variety of collection and analytical methods in an effort to seek an optimized workflow for this specific sample type. Untreated and treated archived latent fingerprints were utilized to compare different biological sampling techniques, swab diluents, DNA extraction systems, DNA concentration practices, and post‐amplification purification methods. Archived latent fingerprints disassembled and sampled via direct cutting, followed by DNA extracted using the QIAamp® DNA Investigator Kit, and concentration with Centri‐Sep? columns increased the odds of obtaining an STR profile. Using the recommended DNA workflow, 9 of the 10 samples provided STR profiles, which included 7–100% of the expected STR alleles and two full profiles. Thus, with carefully selected procedures, archived latent fingerprints can be a viable DNA source for criminal investigations including cold/postconviction cases. 相似文献
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Abel G. Aganbegyan Ed A. Hewett Abram Bergson Gregory Grossman Herbert S. Levine Jan Vanous 《后苏联事务》2013,29(4):277-297
Three basic directions of the economy's perestroyka (social orientation, uskoreniye of technological readjustment, and restructuring of management in enterprises) are outlined by its leading architect and advisor to General Secretary Gorbachev. The presentation, commencing the first joint Soviet Economy round-table, is followed by questions and comments by American participants relating to sequence, momentum, transition, anticipated consequences and contradictions in the reform. A rejoinder covering such issues as priorities, price formation, adaptation to change, allocation of resources and comparison with the Hungarian and other models, is followed by a concluding exchange of views on competition. Journal of Economic Literature, Classification Numbers: 027, 052, 124. 相似文献
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Ed A. Hewett Valeriy L. Makarov Leonid I. Abalkin Abram Bergson Revold M. Entov Gregory Grossman 《后苏联事务》2013,29(4):332-352
A part of the proceedings of the first joint Soviet Economy roundtable, focused on the pivotal role of modern technology in perestroyka, is presented in the form of questions and answers reflecting a lively debate between distinguished Soviet and American economists. Adaptation to new technologies is analyzed in light of uskoreniye, plan priorities, and a variety of factors influencing economic growth. Also debated are issues relating to enterprises and industries, particularly within the context of contrasts between the civilian and military economies. Specific issues such as the tradeoffs between centralization and autonomy are discussed in some detail. Journal of Economic Literature, Classification Numbers: 052, 124, 621. 相似文献
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Ed A. Hewett 《后苏联事务》2013,29(4):285-305
Gorbachev's economic strategy for the remainder of this decade is analyzed using the information contained in the Twelfth Five-Year Plan covering the years 1986-1990. The plan calls for an acceleration in the rate of economic growth achieved through a modernization of industry, a restructuring of investment, and increased labor effort on the part of the entire labor force. The economic reforms which accompany the plan are fairly modest. Analysis of the plan shows that it is very ambitious, and probably unachievable, although some improvement in economic performance is not excluded. Journal of Economic Literature, Classification Numbers: 052, 113, 124. 相似文献