Brazilian population data on the polymerase chain reaction-based loci LDLR,GYPA, HBGG,D7S8, and Gc

Gene and genotype frequencies in relation to the low density lipoprotein receptor (LDLR), glycophorin A (GYPA), hemoglobin G gammaglobin (HBGG), D7S8, and group specific component (Gc) loci were determined in a sample of 344 unrelated individuals (250 whites and 94 mulattoes) living in the city of S?o Paulo, Brazil. DNA was extracted from 5 mL of peripheral blood obtained from each of the 344 volunteers by the salting-out procedure. Polymerase chain reaction and reverse dot-blot analysis were performed with the Amplitype PM PCR Amplification and Typing Kit (Polymarker Multiplex; Applied Biosystems, Foster City, CA) under conditions recommended by the manufacturer. Estimated allele frequencies in the white sample were in the usual range of that of other United States and European population groups. In any case, genotype distributions for these loci did not deviate significantly from Hardy-Weinberg equilibrium proportions. Only 1 marginally significant (0.01 < P < 0.05) association, between loci HBGG and Gc, was detected in our mulatto sample out of a total of 20 possible pairwise comparisons of the 5 loci for both data sets. Allele frequencies were significantly different (P < 0.001) at the HBGG and Gc loci when the white and mulatto samples were compared. Biologic relationship exclusion probabilities (test powers) were calculated for the data. A Brazilian database has thus been established for the loci LDLR, GYPA, HBGG, D7S8, and Gc, 5 polymerase chain reaction-based loci systems that have been shown to be a useful tool for biologic relationship identification and exclusion.     

TEACHING METHODS IN PUBLIC ADMINISTRATION REVISITED

Methodology courses in MPA programs tend to be unpopular with students and professors alike. These same courses, however, prove to be among the most valuable when the student is completing the degree program or when the student becomes a practitioner of public administration. In this article, many of the most widely used learning models for methodology are critiqued. In addition, the authors make a case for the use of inquiry training techniques, arguing that they are most appropriate for both students and professor because of their environmental sensitivity.     

Jean Devanny

Carole Ferrier (ed), Point of Departure: the Autobiography of Jean Devanny (University of Queensland Press) St Lucia, Queensland, 1986.     

The developmentally handicapped witness

This study compared the ability of 30 developmentally handicapped (DH) and 30 nonhandicapped individuals in their ability to report on witnessed events. Participants watched a film clip and were asked to respond to five different types of questions about it. Misleading or leading questions were embedded in three of the question types Results indicated that in response to free recall and very general questions, DH participants did not provide as complete responses as did nonhandicapped participants, although the information provided by both groups tended to be quite accurate. Both groups, and particularly DH individuals, were less accurate in responding to more focused shortanswer recall questions. In response to correct leading specific and statement questions, requiring recognition of the information provided, DH and control participants were comparable in their performance. DH participants had greater difficulty with misleading recall questions and false leading specific and statement questions. Results are discussed in terms of optimizing eyewitness accuracy in DH individuals.