DNA-based and taxonomic identification of forensically important Sarcophagidae (Diptera) in southeastern Spain

Studying dipterans at the scene of a death can provide essential information for interpreting the evidence and help to reconstruct the events happened to a corpse in the past. Molecular tools have been employed for identification at specific levels in the cases of cryptic species or poorly conserved specimens. Identification of specimens is essential in forensic entomology since each species has a specific growth rate, which determines the calculation of the minimum post mortem interval (minPMI). In addition, phylogeographic reconstruction within a species can help to differentiate the haplotypes from a geographic area, thereby helping to clarify the possible relocation of a corpse. The morphological identification of Sarcophagidae species is often difficult, especially for the females. This is an important Diptera family since some of its species are among the first to reach a corpse, especially in warm areas. In this study, we compared the sarcophagids found in human corpses in forensic cases in Alicante (southeast of Spain) with specimens collected from baited traps in the same area and surrounding provinces. In total, 189 specimens were collected, comprising 72 from forensic cases and 117 from baited traps. Molecular identification was conducted by sequencing the cox1 mitochondrial gene and analyzing the sequences using ABGD, GMYC, and BIN species delimitation methods. The median joining algorithm in the PopART program was used to construct phylogeographic networks. Eight species in the family Sarcophagidae were identified. The most widely collected species were Sarcophaga argyrostoma and Sarcophaga tibialis. The haplotype networks obtained for these species did not indicate a clear geographic distribution of haplotypes. The S. argyrostoma samples from Alcoy were clearly isolated. The results demonstrated that this method is useful for identifying Sarcophagidae samples in forensic investigations and it can be employed for minPMI estimation.     

Associational Patterns of Scavenger Beetles to Decomposition Stages

Beetles associated with carrion play an important role in recycling organic matter in an ecosystem. Four experiments on decomposition, one per season, were conducted in a semirural area in Bahía Blanca, Argentina. Melyridae are reported for the first time of forensic interest. Apart from adults and larvae of Scarabaeidae, thirteen species and two genera of other coleopteran families are new forensic records in Argentina. Diversity, abundance, and species composition of beetles showed differences between stages and seasons. Our results differed from other studies conducted in temperate regions. Four guilds and succession patterns were established in relation to decomposition stages and seasons. Dermestidae (necrophages) predominated in winter during the decomposition process; Staphylinidae (necrophiles) in Fresh and Bloat stages during spring, summer, and autumn; and Histeridae (necrophiles) and Cleridae (omnivores) in the following stages during those seasons. Finally, coleopteran activity, diversity and abundance, and decomposition rate change with biogeoclimatic characteristics, which is of significance in forensics.     

The Application of Flow Cytometry as a Rapid and Sensitive Screening Method to Detect Contamination of Vitreous Humor Samples and Avoid Miscalculation of the Postmortem Interval

Research into maximizing the speed, precision, and reliability of estimating the postmortem interval (PMI) has been a recurring object of investigation and methodologies based on the vitreous humor (VH) have provided good results. However, contamination from causes not readily apparent, such as blood, can occur, and thus lead not only to an erroneous estimation of PMI, but also interfere with the correct identification of other substances in the VH. We have developed a flow cytometry method which quantifies blood contamination and is able to detect erythrocytes in 1:750,000 dilution of contaminated VH which affects the results of hypoxanthine. It is an improvement on the previous more complex mass spectrometry method, being faster, more sensitive, and readily available. As such, it could be proposed for the rapid screening of appropriate samples by detecting and eliminating blood contaminated samples from PMI estimation.     

Prefatory Note

In 1954, the Supreme Court of the United States ruled that segregatedschools were inherently unequal in the historic Brown v. Boardof Education decision. At that time, schools in the South weresegregated by law, and those in many other districts were defacto segregated by either official policy or housing patterns(Clotfelter, 2004). In addition     

The trust “builders” in the technology transfer relationships: an Italian science park experience

This study proposes and assesses the effectiveness of a solution for disseminating trust in the technology transfer services. Focusing on the original experience of a science park located in Northern Italy, we propose a model focused on interface figures capable of conducting a direct dialogue with an enterprise and gaining its trust thanks to their reputation of reliability and expertise, the informal-personalised nature of the contact and their geographic, social, cultural and professional proximity. These factors stem from the features of this particular “boundary spanner” who identifies in the case-study with retired business owners/managers. This solution has proven to be particularly effective for small and medium-sized enterprises that typically show a preference for local and informal contacts within the networks and the need to refer to guide-figures when interacting with external knowledge sources because often they are not used to such scenarios.     

Challenger Quality and the Incumbency Advantage

Most estimates of the incumbency advantage and the electoral benefits of previous officeholding experience do not account for strategic entry by high‐quality challengers. We address this issue by using term limits as an instrument for challenger quality. Studying US state legislatures, we find strong evidence of strategic behavior by experienced challengers. However, we also find that such behavior does not appear to significantly bias the estimated effect of challenger experience or the estimated incumbency advantage. More tentatively, using our estimates, we find that 30–40% of the incumbency advantage in state legislative races is the result of “scaring off” experienced challengers.     

From the Language of Class to the Rhetoric of Development: discourses of ‘nationality’ and ‘ethnicity’ in China

In the People's Republic of China's (PRC) continuous state-building project since its establishment in 1949, the notion of nation and nationality (minzu) has been uninterruptedly utilised by the leading elite in its political programmes. The notion of the ‘nationality question’ (minzu wenti) was employed especially for addressing the issues of the officially identified minority nationalities at the time when the multinational nature of the state was made fundamental. However, how a multinational character of the Chinese state is interpreted by the state elite and how this interpretation is shaped by their policies have arguably changed along with the political and economic preferences of the regime. This paper aims to explore the meaning of ‘nationality question’ during the period of socialist construction, and traces the shift in its notion during the reform period which started in the 1970s. The essay pays attention to the change in the preferred corresponding English term of minzu wenti. I argue that the substitution of the ‘nationality question’ term, which was dominant during the socialist period, with the concept of the ‘ethnic question’ during the reform period points not only to the changing character of the government policies towards minorities, but also legitimises them as the only suitable and rightful in the context of economic reforms.     

Can Facebook really be a tool for supporting citizen engagement during emergencies?

The present study explores how Italian regions and their presidents employed Facebook during the Covid-19 pandemic to encourage citizen engagement. To do so, it adopts quantitative methodologies. Our results show that most public actors increased their levels of social activity during the pandemic but mainly for promoting public communication and that citizens much prefer interacting with presidents. Moreover, citizen engagement was higher when posts were published during nonbusiness hours or weekends, while it decreased when posts contained photos or videos.