Messenger RNA profiling: a novel method for body fluid identification by real-time PCR

Conventional methods for the identification of different body fluids like blood, semen and saliva from biological stains involve immunological or enzymatic detection of certain proteins. In this study, we investigated potential RNA markers with the aim of developing Real-Time polymerase chain reaction (PCR) based methods to allow differentiation between several body fluids. Total RNA samples from artificially stained swabs and from various pieces of evidence from case work were extracted, amplified and analyzed with several RNA markers. Three assays detecting the body fluids of interest were selected: hemoglobin-alpha locus 1 (HBA), kallikrein 3 (KLK) and mucin 4 (MUC). With this approach, we demonstrate that specific Real-Time PCR assays are useful in identifying the source of the biological stain. Furthermore, RNA profiling of various body fluids was even possible on samples stored over a long period of time at ambient temperature. The stability and sensitivity of the applied method outlines a novel application for Real-Time PCR within the forensic field.     

Hispanic perception of police performance: An empirical assessment

This exploratory study examines relationships between the police and Hispanics in Texas. Specifically, data were collected on Hispanic evaluations of police performance, expectations of police performance, and overall satisfaction with the police. A sample of 500 Hispanics throughout the state of Texas were surveyed on their experienced and perceived relationships with three levels of police agencies: local (municipal) police, sheriff's officers, and the state's Department of Public Safety. Findings are reported based upon overall ratings of the police, victim-related data, and data from individuals who had any form of contact with the police. Significant findings show that any form of contact with the police appeared to lower the rating of police performance. This appears to be a product of the interaction between high public expectations and qualitatively poor police performance. Further findings indicate that an increase in the fear of crime among Hispanics lowered evaluations of local police. Similarly, victimization lowered the evaluation of local police, the county sheriff, and the Department of Public Safety. Hispanics also perceived that officers have a “bad attitude,” that the police need to patrol and investigate more, that response time should be improved, and that there should be less discrimination against Hispanics. These findings appear to be largely influenced by ineffective communication (both symbolic and linguistic) and cultural conflict.     

Justice Beliefs in School: Distinguishing Ultimate and Immanent Justice

This paper combines two recent developments in just world research: the conception of belief in a just world (BJW) as a resource for coping and well-being in everyday life, and the distinction between two different facets of BJW, namely belief in immanent justice (BIJ) and belief in ultimate justice (BUJ). Moreover, these two aspects are adapted to the school context and educational psychology. Scales for measuring ultimate and immanent justice in schools were developed within a German pilot reform project with a sample of 1274 pupils. The two facets could be distinguished by means of factor analysis. The reliability of the two scales is satisfactory, and their validity is confirmed by their differential correlational patterns. Results show that not every form of BJW automatically functions as a coping resource. In fact, only BUJ is able to protect pupils' mental well-being, whereas immanent justice can actually jeopardize well-being. Similarly, BUJ is associated with the perception of solidarity and learning enjoyment in class, whereas BIJ is more closely associated with the experience of rivalry and competition in class. Finally, the socialization of the two justice beliefs is considered. The cross-sectional data provide some evidence to suggest that friendly and supportive parenting styles can promote BUJ, whereas strict parenting styles may further BIJ. More longitudinal research is needed to obtain further insights into these phenomena.     

Brothers’ Keepers?

Relying on rarely analyzed public opinion data from the 1930s and early 1940s, we take issue with the notion popular in contemporary liberal circles that the New Deal era represented a period of expansive commitment to the security and well-being of the poor and politically disenfranchised. At least where the public is concerned—as opposed to the progressive policy makers in the Roosevelt administration—the jobless were regarded with suspicion, immigrants should be forced to “go home,” women belong in the kitchen not on the shop floor. The harsher the economic conditions (by state), the more conservative were the public attitudes. Hence New Deal legislative victories accrued despite rather than because of public support.

Assessing the psychological and mechanical impact of electoral rules: A quasi-experiment

The paper assesses the influence of electoral rules on vote choice and election outcomes using a quasi-experiment conducted during a recent Canadian provincial election. Respondents were invited to vote under three voting systems (first past the post, alternative voting and proportional representation) and to answer a short questionnaire. We examine how the distribution of votes and seats is affected, and we ascertain how much of the total difference is due to psychological and mechanical effects. We find that a PR system would have increased legislative fractionalization by the equivalent of one effective party and that the mechanical effect is much more important than the psychological effect. As for AV, its mechanical and psychological effects act in opposite directions.     

Constructing “The Same Rights With the Same Names”: The Impact of Spanish Norm Diffusion on Marriage Equality in Argentina

This article argues that Spain has been the driving external force in the advancement of LGBT rights in Latin America, from marriage in Argentina to the regional recognition of “sexual diversity rights” as human rights. Acting as “norm entrepreneurs,” Spanish activists and organizations, relying on development aid, have promoted their perspectives through two approaches: strategic consulting and resource transfer. Their diffusion is illustrated primarily by the Argentine case. There, activists underwritten by Spanish resources have borrowed Spanish strategies to achieve “the same rights with the same names.” Besides broadening our understanding of the struggle for LGBT equality in Latin America, this article deepens the explanation of norm diffusion, focusing on emergence. In this stage, specific individuals and organizations deliberately select appropriate “targets” for and moments of intervention. But norm “receptors” must also be ready for action.     

Potential carcass enrichment of the University of Tennessee Anthropology Research Facility: A baseline survey of edaphic features

The University of Tennessee Anthropology Research Facility (ARF) is known for its unique contribution to forensic science as a site of human decomposition research. Studies conducted at ARF are integral in our understanding of the processes of human decomposition. As such, the authors are interested in the long-term effects of continuous human decomposition on the soil environment. Soil samples collected from within and outside the ARF were evaluated for moisture content, pH, organic content, total carbon and nitrogen content, and biomass by lipid-bound phosphorus, and total extracted DNA. Analyses revealed no significant differences (p<0.05) among the sampled areas within the facility, and yet demonstrated a possible trend toward increased levels of total N, Lipid-P, and water, suggesting an influx of high-quality nutrients into the ARF soil. Furthermore, elevated pH readings, presumably resulting from ammonification of the soil, were observed in areas of high decomposition. The negative control samples proved significantly different from nearly all samples collected within the facility, the exceptions being total carbon content and extractable DNA. These findings indicate that while landscape samples inside may be similar to themselves, they are dissimilar to those taken in a similar temperate forest biome with no recorded history of human decomposition.     

Does Self-Control Influence Maternal Attachment? A Reciprocal Effects Analysis from Early Childhood Through Middle Adolescence

Objectives

The purpose of this study is twofold. First, this study assesses the extent to which self-control and maternal attachment mutually influence one another. Second, it investigates whether this process continues to occur during adolescence. To date, studies of the etiology of self-control have yet to adequately address these issues, despite the fact that a number of theoretical perspectives emphasize the reciprocal nature of the parent-child relationship.

Methods

The current study seeks to shed light on these issues by examining the relationship between self-control and maternal attachment using structural equation modeling for eight waves of data spanning a period of time that encompasses early childhood through middle adolescence.

Results

The results yield two findings bearing on the adequacy of Gottfredson and Hirschi??s model of self-control development. First, measures of self-control and maternal attachment were found to mutually influence one another during childhood. Second, these effects were reduced to nonsignificance during adolescence.

Conclusions

This study finds that self-control emerges during childhood in a complex manner in which it both shapes and is shaped by parental attachment.