Fixation of cells for analysis by laser microdissection--comparative studies in forensic trace material

This paper is focused on the preparation of samples for laser microdissection (LM) in forensic casework. In forensic genetics, it is essential to preserve and separate cellular traces during sample preparation, as they are usually gathered in very small amounts and are often contaminated with undesired cells. This is made possible by laser microdissection, a technique developed to cut cells or tissue of a certain type from a microscopical specimen by UV laser and catapult them directly into a PCR reactor. This method minimizes the risk of getting inconclusive, mixed DNA profiles due to contamination by foreign DNA and also supplies information about the cellular origin of a DNA profile. A method for optimized fixation and staining of spermatozoa for laser microdissection was established. Four different fixation methods combined with two staining methods were tested on two different microscope slides. Moreover, the effect of a blocker pen to contain the specimen on the slide was investigated.     

Literatur

Ohne Zusammenfassung     

Issue Importance and Performance Voting

Issue importance mediates the impact of public policy issues on electoral decisions. Individuals who consider that an issue is important are more likely to rely on their attitudes toward that issue when evaluating candidates and deciding for whom to vote. The logic behind the link between issue importance and issue voting should translate to a link between issue importance and performance voting. Incumbent performance evaluations regarding an issue should have a stronger impact on the vote choice of individuals who find that issue important. The analysis demonstrates that there is a significant interaction between performance evaluations and issue importance. People concerned about an issue assign more weight to their evaluations of the government's performance on that issue when making up their mind.     

Is Inspiring Group Members an Effective Predictor of Social Dominance in Early Adolescence? Direct and Moderated Effects of Behavioral Strategies,Social Skills,and Gender on Resource Control and Popularity

Dominance in the peer group is important for adolescents. Resource Control Theory posits that both coercive and prosocial (positively assertive) strategies are associated with dominance. Combining Resource Control Theory with Socioanalytic Theory on personality, we hypothesized that inspiring group members would be an additional effective strategy. This study examined whether the three behavioral strategies and two types of social skills (social competence and manipulation) predicted dominance (resource control and popularity). Participants were 619 Dutch adolescents (M_age?=?13.1; 47% female) in the first grade of secondary school. They completed peer reports (behavioral strategies and dominance) and self-reports (social skills). Only inspirational and coercive strategies substantially predicted dominance. Main effects of social skills emerged. Moderation between strategies and social skills was only observed for girls (e.g., coercive strategy use was associated with more popularity for girls with higher levels of social manipulation skills). This study furthered our understanding of the predictors of dominance in adolescence by including inspirational behavior and examining prosocial and antisocial skills.     

Feminism and Constructivism: Worlds Apart or Sharing the Middle Ground?

The discipline of international relations (IR) is witnessing a "constructivist turn." In this article, we argue that the new preoccupation with constructivism provides a unique opportunity to further understanding between feminism and the IR mainstream. Feminism and constructivism share a commitment to an ontology of becoming that can serve as a common basis for conversation. Yet there are also profound differences between feminists and constructivists. First, most IR feminists approach gender and power as integral elements in processes of construction, whereas most constructivists consider power to be external to such processes. This failure to conceptualize power and gender as social and pervasive leads constructivists to miss an important part of the empirical reality of power politics. Second, constructivists tend to ignore the implications of a postpositivist epistemology, whereas for feminists the question of "Who knows?" is crucial. We argue that the constructivist failure to problematize the research process as a social (and therefore political) process of construction is logically inconsistent with an ontology of becoming. We introduce empirical materials to illustrate the advantages of feminist approaches. We hope to advance a dialogue between feminism and constructivism because the two approaches add to each other and in combination can yield better theoretical and empirical understandings of the world.     

Rechtsanwälte,Disziplinarrecht, Werbung,Meinungsäußerungsfreiheit

Die zugrundeliegende Disziplinarstrafbestimmung ist hinreichend bestimmt. Eine demokratische Gesellschaft kann die inkriminierte Werbung hinnehmen, ohne dass der Schutz des guten Rufes oder das Ansehen und die Unparteilichkeit der Rsp Schaden erleiden. Die Disziplinarstrafe verletzt die Bf im Grundrecht auf Meinungs?u?erungsfreiheit, indem die bel Beh dem Gesetz einen verfassungswidrigen Inhalt unterstellt hat.     

Reviews

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