Informativeness of NGS Analysis for Vaginal Fluid Identification

The identification of vaginal fluids in forensic examinations plays an important role in crime scene reconstruction. Molecular detection of vaginal bacterial communities can lead to the correct discrimination of body fluids. These kinds of studies can be performed through multiplex real‐time PCR using primers for a specific selection of bacteria. The availability of next‐generation sequencing (NGS) protocols provided for the extension of the analysis to evaluate the prokaryotes present in specimens. In this study, DNA was extracted from 18 samples (vaginal, oral, fecal, yoghurt) and analyzed by real‐time PCR and NGS. The comparison between the two approaches has demonstrated that the information developed through NGS can augment the more conventional real‐time PCR detection of a few key bacterial species to provide a more probative result and the correct identification of vaginal fluid from samples that are more forensically challenged.     

Choosing the scope of trade secret law when secrets complement patents

We present a model where an incumbent firm has a proprietary product whose technology consists of at least two components, one of which is patented while the other is kept secret. At the patent expiration date, an entrant firm will enter the market on the same technological footing as the incumbent if it is successful in duplicating, at certain costs, the secret component of the incumbent's technology. Otherwise, it will enter the market with a production cost disadvantage. We show that under some conditions a broad scope of trade secret law is socially beneficial.     

Reviews

Pekka Sutela, Economic Thought and Economic Reform in the Soviet Union. Cambridge: Cambridge University Press, 1991, 197 pp., £30.00 h/b, £10.95 p/b.

Stephen White, Gorbachev and After. Cambridge: Cambridge University Press, 1991, ix+310 pp., £27.95 h/b, £8.95 p/b.

Catherine Merridale & Chris Ward, eds, Perestroika. The Historical Perspective. London, New York, Melbourne, Auckland: Edward Arnold, 1991, xiii+253 pp., £12.95 p/b.

Guy Standing, ed., The New Soviet Labour Market. In Search of Flexibility. Geneva: ILO, 1991, xiv+440 pp., SF45.00.

Leonard Geron, Soviet Foreign Economic Policy under Perestroika. London: Chatham House Papers, The Royal Institute of International Affairs, Pinter Publishers, 1990, 126 pp., £19.50, h/b, £7.95 p/b.

Malcolm R. Hill, Soviet Advanced Manufacturing Technology and Western Export Controls. Aldershot: Avebury, 1991, xv+256 pp., £35.00.

Gerhard Simon, Nationalism and Policy toward the Nationalities in the Soviet Union: From Totalitarian Dictatorship to Post‐Stalinist Society, translated by K. and O. Forster. Boulder, CO, and Oxford: Westview Press, 1991, xvii+483 pp., £22.50 p/b.

A. Kemp‐Welch, Stalin and the Literary Intelligentsia 1928–39. London: Macmillan, 1991, vi + 338 pp., £45.00.

Jelena Milojkovic‐Djuric, Aspects of Soviet Culture: Voices of glasnosf, 1960–1990. New York: East European Monographs, Columbia University Press, 1991, iv+190 pp., $29.00.

Landon Pearson, Children of Glasnost. Growing up Soviet. Seattle, WA: University of Washington Press, 1991, xv+505 pp., $16.95, p/b.

Robert Rand, Comrade Lawyer. Inside Soviet Justice in an Era of Reform. Oxford: Westview Press, 1991, x+166 pp., £9.50 p/b.

Sheila Fitzpatrick, Alexander Rabinowitch & Richard Stites, eds, Russia in the Era of NEP: Explorations in Soviet Society and Culture. Bloomington and Indianapolis, IN: Indiana University Press, 1991, viii+344 pp., £22.50 h/b, £9.50 p/b.

Efraim Karsh, Soviet Policy towards Syria since 1970. Basingstoke and London: Macmillan, 1991, 229 pp.+index, £35.00.

Ronald D. Bachman, ed., Romania: A Country Study, 2nd edition. Washington, DC: US Library of Congress Federal Research Division, 1991, xxxvi+356 pp.     

Building resilient supply chains in uncertain times: a comparative study of EU and ASEAN approaches to supply chain resilience

Asia Europe Journal - Amid rising uncertainty in the global economy and unprecedented economic disruption caused by the COVID-19 pandemic, the concept of supply chain resilience has gained...     

Evaluation of four oral fluid devices (DDS®, Drugtest 5000®, Drugwipe 5+® and RapidSTAT®) for on-site monitoring drugged driving in comparison with UHPLC-MS/MS analysis

New Italian legislation on driving under the influence of drugs considers oral fluid (OF) as a possible alternative drug testing matrix. On this basis, the present research was carried out to evaluate the applicability of four commercial on-site OF drug screening devices, namely DDS(?), Drugtest 5000(?), Drugwipe 5+(?) and RapidSTAT(?), in a real operative context. Preliminarily trained police officers tested randomly stopped drivers with two different kits side-by-side during roadside patrols. A central laboratory confirmed on-site kits' results by UHPLC-MS/MS analysis of the saliva specimen remaining after the screening analysis. 1025 drivers were submitted to the OF tests: 11.6% were positive for cocaine and metabolites, 11.1% for THC, 6% for amphetamines and amphetamine-type designer drugs and 2.3% for ketamine. The sensitivities of the kits were 81% (RapidSTAT(?)), 82% (DDS(?)), 90% (Drugwipe 5+(?)) and 97% (Drugtest 5000(?)) for cocaine and 38% (DDS(?)), 47% (Drugwipe 5+(?)), 72% (RapidSTAT(?)) and 92% (Drugtest 5000(?)) for THC. Drugtest 5000 was the only kit showing an acceptable sensitivity for on-site application. Only Drugtest 5000(?) and RapidSTAT(?) could be evaluated for amphetamines and methamphetamines: Drugtest 5000(?) showed a sensitivity of 100% in the case of amphetamines and 86% for methamphetamines, while RapidSTAT(?) 90% and 76% respectively. Nowadays, ketamine is not included in the target analytes of any on-site devices, but it was systematically included in the UHPLC-MS/MS confirmatory analysis. To ensure adequate reliability, MS confirmation of on-site OF screening tests is anyway always necessary, due to the presence of a significant number of false positive results even when using the commercial kit with the best performance.     

Are non-traditional security challenges leading regional organizations towards greater convergence?

How are regional organizations responding to the emergence of non-traditional security (NTS) challenges? Are they engaging in more cooperative efforts to meet new threats? Or, on the contrary, do they react in different manners according to their distinctive values, principles and internal structures? This article attempts to investigate how the threats posed by NTS are compelling different regional organizations to reconsider their security thinking and to find new innovative ways of cooperation. This is done by comparing two diverse regional organizations, the EU and the Association of Southeast Asian Nations (ASEAN), whose models of security cooperation have significantly varied reflecting the preference for different security approaches. The EU's security system has been more formalized and institutionalized; conversely, the "ASEAN way" has traditionally been rooted in the principles of informality and consensus. It is argued, however, that the emergence of NTS threats is acting as a catalyst behind a normative and operational shift of the modus operandi of both organizations. In so doing, this empirical analysis will try to shed light on the effects of exogenous factors on the emergence of patterns of convergence within the security sphere of distinctive regional processes.     

Styling the self online: semiotic technologization in weblog publishing

ABSTRACT

Testing on digital semiotic production the concepts of (self-)styling and technologization of discourse, developed for offline linguistic phenomena, the article investigates the role of digital platforms in shaping the relation between self-expression online, semiotic regulation and the social construction of taste. By focusing on the use of semiotic resources of webdesign for identity expression, the study analyses the semiotic regimes emerging from regulatory practices and webdesign styles on WordPress, and their influence on the changes in the projected identity of a personal blogger. In spite of the participatory character of WordPress, results show the role of the platform in objectifying/technologizing hegemonic semiotic preferences, with consequent normalising effects in bloggers’ self-styling practices. The conclusions relate the findings to broader power dynamics in the social construction of taste and their implications for both online and offline forms of self-expression.