大熊猫轮状病毒CH-1株外衣壳蛋白VP4基因的克隆与生物信息学分析

利用MA-104细胞培养增殖大熊猫轮状病毒CH-1株,提取总RNA,运用RT-PCR扩增外衣壳蛋白VP4基因,将其与pMD19-T simple vector连接并转化DH5α,经PCR扩增和测序分析进行鉴定,用生物信息学软件预测其功能,并构建系统进化树。结果显示,成功获得大熊猫轮状病毒VP4蛋白基因,长2 362bp,包含一个2 331bp的开放阅读框,编码776个氨基酸,测序结果在GenBank中的登录号为HQ641296。生物信息学分析表明,VP4蛋白基因编码产物分子质量为86 768.8u,理论等电点为5.56,半衰期为30h(体外哺乳类网状细胞),不稳定系数为28.64,脂肪指数为82.53,总平均亲水性为-0.265,最大疏水性为2.244,最小疏水性为-2.911;无信号肽序列;跨膜结构分析显示VP4蛋白无跨膜区,位于病毒膜外区;在VP4序列中的抗原决定簇主要位于VP4的N-末端和C-末端。预测其可能包含8个N-糖基化作用位点,15个蛋白激酶C磷酸化作用位点、15个酪蛋白激酶Ⅱ磷酸化作用位点、12个N-豆蔻酰化位点。系统进化分析显示,大熊猫轮状病毒的VP4基因与猪轮状病毒的VP4基因的进化距离最...     

人体血液体外电击致血细胞穿孔的扫描电镜观察

目的探讨血细胞体外电击时细胞膜的结构变化。方法对成人血液分别分组通过220V交流电电击5,10,20,30s和1min后,用扫描电镜观察血细胞膜的变化。结果电击后,即可以观察到红细胞与白细胞的细胞膜穿孔,其中红细胞穿孔可为一个或多个,形状多为圆形或卵圆形,白细胞穿孔则为多个,呈筛网状;穿孔细胞数随电击时间延长而增多;电击5s时为6%左右,以后逐渐增加,至电击1min时,穿孔细胞数可达40%左右。结论电流作用可以使血细胞膜发生穿孔,穿孔细胞数随通电时间延长而增多。     

Reducing intimate partner homicides: The effects of federally-funded shelter service availability in California

Over twenty-five years of policy and scholarly attention to the problem of intimate partner violence appears to have generated greater victim safety. Intimate partner homicides, the most serious form of violence between intimates, have been declining for nearly three decades in many contexts, but not all. This study built on a small number of studies that had advanced knowledge about the policy-relevant factors behind the intimate partner homicide decline. The analysis described here utilized a set of county-level data from California from 1987 to 2000 to estimate the effects of shelter-based service availability on race/ethnicity-specific female intimate partner homicide victimization. The study relied on a new measure of service availability in an attempt to overcome limitations with previously used indicators. This analysis did not reveal an effect of shelter-based service availability and criminal justice system responses on changes in female intimate partner homicide victimizations. Reasons for these findings and suggestions for future research are discussed.     

昆明地区复方地芬诺酯片滥用情况分析

目的:了解昆明地区复方地芬诺酯片的滥用情况。方法:自行设计调查表,对2006年1月-2006年5月入所发现的复方地芬诺酯片滥用者进行调查。结果:在2152例海洛因滥用者中共发现复方地芬诺酯片滥用者208例,占9.67%。在208例复方地芬诺酯片滥用者中,男162例,占77.88%,女46例,占22.12%;年龄17-50(33.5±s6.2)a;婚姻状况:未婚134例,占64.42%,同居24例,占11.54%,已婚29例,占13.94%,离异21例,占10.10%;文化程度:小学84例,占40.38%,初中105例,占50.48%,高中及以上19例,占9.13%;职业:无业174例,占83.65%,个体15例,占7.21%,驾驶员8例,占3.85%,服务人员5例,占2.40%,其他6例,占2.88%。滥用复方地芬诺酯片的主要方式:口服(100%);初始滥用的主要原因:"戒毒"(49.04%),目前滥用的主要原因:"替代海洛因"(46.63%);主要来源:药店(83.66%)和小诊所(47.52%);有19例(9.13%)曾发生过滥用复方地芬诺酯片急性中毒。结论:昆明地区药物滥用者中出现复方地芬诺酯片滥用情况;复方地芬诺酯片容易获得;滥用的主要原因与海洛因滥用有关;希望有关部门予以重视。     

Crisis and vulnerability in adolescent development

It was argued that adolescent development should be studied from a more relational and structural approach (cf. Damon and Hart, 1982). In the first study of 5986 Chinese students, two distinct self-concept dimensions were included and their relations to one another and to locus of control, extraversion, and test anxiety were compared across six primary and secondary grade levels. This was in contrast to past studies' focus on the overall change of single or global aspects of self-concept. It was found that self-concept of academic ability increased with age, whereas self-concept of appearance decreased with age. Both self-concepts were closely related. Locus of control was more related to self-concept appearance, and test anxiety to self-concept of academic ability. Extraversion was related only to self-concept of appearance. These relations existed mainly in adolescents (especially girls) and not in younger children. Midadolescence was found to be a critical period as both self-concepts showed quite drastic changes. Tentative evidence showed that the transition from sixth to seventh grade tended to have a dampening effect on the two self-concepts. Results from a second study of 701 secondary school students that included more self-concept dimensions lent further support to the findings of the first study. Crisis and vulnerability in adolescent development are discussed in the qualitative analysis of the relational changes.Ph.D. in social psychology, Purdue University, West Lafayette, Indiana. Research interests: Value and self-concept development, parental infuences, and deviant behaviors.     

The attentive spectators: Political participation of the Hong Kong Chinese

Based on a large-scale territory-wide questionnaire survey, this study purports to delineate the pattern of political participation of the Hong Kong Chinese. It is found that though the overall level of participation remains low, political activism has nonetheless increased since the early 1980s. Political participation in Hong Kong is primarily parochial and conventional in nature, yet there also exists a significant expressive and unconventional component. The mode of participation is fragmented and largely individualistic. Hong Kong Chinese are increasingly inclined to take collective actions, but participation mobilized by political groups is still limited. Hong Kong Chinese however pay much attention to politics. In view of the coexistence of high cognitive participation and low behavioral participation, Hong Kong Chinese can be appropriately described as “attentive spectators.” He is the author ofSociety and Politics in Hong Kong (1982) andHong Kong Politics in the Transitional Period (in Chinese, 1993)     

Construct accessibility and electoral choice

Chronic accessibility refers to a long-term bias to notice, process, and have available for recall certain types of information across a variety of different stimulus objects in a variety of different situations. This paper illustrates the usefulness of studying the chronic accessibility of political constructs in the field of political behavior. The chronic accessibility of four generic political constructs are operationalized: candidates, issues, groups, and parties. The accessibility of these four political constructs is shown to be relatively stable over time and to guide the processing of information about a wide variety of political objects. Next, a voting model is tested that identifieswhich voters will rely chiefly on issue orientations, group orientations, candidate orientations, and/or party orientations in making their vote decision. The voting model is validated across two distinct ways of operationalizing the political chronicities and three different election studies spanning a 28-year period. Finally, although this paper has focused onindividual political behavior, several ways that an information processing approach could shed light on macrolevel political questions are discussed.     

检警一体化与检察指导侦查机制比较研究

检警一体化是大陆法系国家一种比较成熟的刑事犯罪追诉模式 ,检察指导侦查则是我国理论界和司法实务界所积极探索与实践的一种刑事犯罪追诉创新机制。检警一体化机制与检察指导侦查机制的相同之处在于提高刑事司法效率、查清案件事相、预防侦查违法和防止诉讼偏差 ;不同之处在于产生的法律制度和理论背景不同、检察官的主导作用和对犯罪案件的决定权限不同、侦查主体的能动性发挥不同