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101.
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Christina Valgren Erika Edenberger 《Forensic Science International: Genetics Supplement Series》2008,1(1):78-79
Differential extraction is an efficient method to separate sperm cells from epithelial cells. A manual Chelex®-100 based method is used at the Swedish National Laboratory of Forensic Science, SKL. The Differex™ System (Promega) uses a Proteinase K digestion of epithelial cells followed by centrifugation and phase separation. The sperm- and epithelial fractions are further purified with DNA IQ™ System (Promega) or with phenol/chloroform. The Differex™ System in combination with DNA IQ™ System were evaluated and compared to the Chelex®-100 method used routinely. After modifications, the Differex™ System gave comparable results to the Chelex®-100 method. The modifications included additional Proteinase K and DTT, longer incubation time and additional steps when removing the solid support from the Digestion Solution. In the Chelex®-100 based method microscopic examination is done on the sperm pellet in a total volume of 50 μl. It was not possible to do a microscopic examination in less than 100 μl using the Differex™ System. Additionally the sperms were in clusters of epithelial cell debris. Microscopic examination is an important part of the differential extraction at SKL. Therefore, the Differex™ System will not be implemented at our laboratory. 相似文献
103.
Abstract:
An alarming number of drownings occur in lifeguarded swimming areas, where one might presume swimmers are protected from injury. One reason drownings occur in lifeguarded swimming areas is because lifeguard surveillance is a highly difficult task. Observational research suggests lifeguards are usually alert, but researchers also report egregious examples of inattention. We offer three strategies that have initial empirical support to reduce risk of drowning at lifeguarded swimming areas: (a) regular training to help lifeguards recognize they are vulnerable to drowning events and to raise their confidence; (b) regular practice via simulated emergency responses, and (c) addressing staff schedules so lifeguards can devote full attention to protecting swimmer safety while on duty. 相似文献104.
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POLICY MODELING TO SUPPORT ADMINISTRATIVE DECISIONMAKING ON THE NEW YORK STATE HIV TESTING LAW
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Erika G. Martin Roderick H. MacDonald Lou C. Smith Daniel E. Gordon James M. Tesoriero Franklin N. Laufer Shu‐Yin J. Leung Daniel A. O'Connell 《Journal of policy analysis and management》2015,34(2):403-423
A recent New York law requires medical providers to offer HIV tests as part of routine care. We developed a system dynamics simulation model of the HIV testing and care system to help administrators understand the law's potential epidemic impact, resource needs, strategies to improve implementation, and appropriate outcome indicators for future policy evaluations once postlaw data become available. Policy modeling allowed us to synthesize information from numerous sources including quantitative administrative data sets and practitioners’ content expertise, structure the information to be viewed both numerically and visually, and organize consensus for decisionmaking purposes. This case illustrates how policy modeling can provide an integrated framework for administrators to examine policy problems in complex systems, particularly when data time lags limit pre–post comparisons and key outcomes cannot be measured directly. 相似文献
107.
Portillo Shannon Sexton Lori Smith Sarah M. Dinsmore Renee Reyes Erika Garcia 《Crime, Law and Social Change》2022,77(2):159-184
Crime, Law and Social Change - Justice as a concept has permeated sociolegal scholarship in the U.S. since the founding of Law and Society as a field in the mid 1960s. Much of this scholarship has... 相似文献