Multiplex PCR and minisequencing of SNPs--a model with 35 Y chromosome SNPs

We have developed a robust single nucleotide polymorphism (SNPs) typing assay with co-amplification of 25 DNA-fragments and the detection of 35 human Y chromosome SNPs. The sizes of the PCR products ranged from 79 to 186 base pairs. PCR primers were designed to have a theoretical Tm of 60 +/- 5 degrees C at a salt concentration of 180 mM. The sizes of the primers ranged from 19 to 34 nucleotides. The concentration of amplification primers was adjusted to obtain balanced amounts of PCR products in 8mM MgCl2. For routine purposes, 1 ng of genomic DNA was amplified and the lower limit was approximately 100 pg DNA. The minisequencing reactions were performed simultaneously for all 35 SNPs with fluorescently labelled dideoxynucleotides. The size of the minisequencing primers ranged from 19 to 106 nucleotides. The minisequencing reactions were analysed by capillary electrophoresis and multicolour fluorescence detection. Female DNA did not influence the results of Y chromosome SNP typing when added in concentrations more than 300 times the concentrations of male DNA. The frequencies of the 35 SNPs were determined in 194 male Danes. The gene diversity of the SNPs ranged from 0.01 to 0.5.     

Quality of Laypersons' Assessment of Forensically Relevant Stimuli,

The current study examined the potential for cognitive bias in lay examiners' comparisons of footwear impressions within the technical review process while addressing limitations of previous research. Prior research has found inconsistent results regarding the extent to which cognitive bias may influence forensic comparisons, often asking non-experts to review forensic stimuli above their competency level. Furthermore, past research has largely ignored the potential for cognitive bias during the technical review process. In collaboration with the Miami-Dade Police Department's Forensic Services Bureau, we examined the effects of previous examiners' level of experience and prior knowledge of the previous examiner's decision on the technical review stage of footwear impression stimuli. Before lay examiners were presented with pairs of known match and nonmatch footwear impressions, they were either told that an expert or a novice had previously examined them and determined them to be either a match, nonmatch, or inconclusive (plus a no-information condition). Participants then evaluated each pair of footwear impressions to make their own determinations of match, nonmatch, or inconclusive. Results support the technical review process for all decision types, as known nonmatch stimuli were generally more difficult for lay examiners to assess than known match stimuli. Knowledge of a prior examiner's decision and status was observed only when the prior decision was inconclusive, suggesting the need for inclusion of inconclusive decisions in future research examining cognitive bias in forensic examination.     

IMF flexibility or neoliberal adaptation: A discursive content analysis of Article IV policy biases in Argentina

At the turn of the century, the consensus among scholars was that the International Monetary Fund's (IMF) policy preferences, which centered on deflation via austerity, privatization, and deregulation, were indicative of its neoliberal bias. However, a subsequent wave of literature has challenged this view by suggesting that the IMF has demonstrated flexibility. While these accounts arrive at their conclusion via different analytical and empirical focuses, this article posits that the flexibility or breath of ideas found within the discourse itself is key to gauging policy biases (previous study). Herein, this article contributes to the question of whether the IMF can be considered a “flexible” institution via its analysis on Argentina (1989–2006; 2016–2017). Extending a previous study's methods, this one provides a “discursive content analysis,” on the IMF–Argentine Article IV consultations. Ultimately, the findings show that policy discourse remained neoliberal throughout.     

‘Light Weapons’ and the Dynamics of Art 47 EU – The EC's Armoury of Ever Expanding Competences

This case note analyses the constitutional scope of the recent Luxembourg ruling of Light weapons and small arms, Case 91/05, Commission v Council delivered on 20 May 2008 (Grand Chamber).     

A "politically robust" experimental design for public policy evaluation, with application to the Mexican universal health insurance program.

We develop an approach to conducting large-scale randomized public policy experiments intended to be more robust to the political interventions that have ruined some or all parts of many similar previous efforts. Our proposed design is insulated from selection bias in some circumstances even if we lose observations; our inferences can still be unbiased even if politics disrupts any two of the three steps in our analytical procedures; and other empirical checks are available to validate the overall design. We illustrate with a design and empirical validation of an evaluation of the Mexican Seguro Popular de Salud (Universal Health Insurance)program we are conducting. Seguro Popular, which is intended to grow to provide medical care, drugs, preventative services, and financial health protection to the 50 million Mexicans without health insurance, is one of the largest health reforms of any country in the last two decades. The evaluation is also large scale, constituting one of the largest policy experiments to date and what may be the largest randomized health policy experiment ever.     

Introduction

SUMMARY

Previously homeless women (17) and men (12) were surveyed on their experience as a caregiver to physically challenged senior citizen clients (16 men, 13 women: age M= 64.7, SD = 16.8). Caregivers had training in service delivery and experience before the survey. More satisfaction than stress from providing care was reported by caregivers. Caregiving satisfaction was significantly positively related to global life satisfaction and a sense of personal vulnerability to being disabled. Caregiving stress was significantly negatively related to perceived knowledge of the disability. Overall, these previously homeless caregivers reported that providing care to the disabled was positive, and would be repeated and recommended to Others.     

Remitting subjects: migrants,money and states

Abstract

Scholars’ and policy-makers’ interest in the remittances that migrants send home to relatives has increased dramatically in the past two decades. Focusing on remittances from the United States to El Salvador, we examine how academic studies, public discourse, and state accounting practices simultaneously produce and reveal the nature of this phenomenon. By treating the money that migrants send home as both national resource and foreign currency, central banks and international financial institutions define remittances as a ‘cost-free’ source of national income. Further, debates about remittances’ social and economic impact focus on whether remitting promotes or undermines particular values associated with neoliberalism, such as self-sufficiency, entrepreneurship and empowerment. Our analysis thus sheds light on new configurations through which money, states and migrant citizens are linked.     

Global History: A View from the South

John Bellamy Foster, MARX'S ECOLOGY: MATERIALISM AND NATURE (New York: Monthly Review Press, 2000)

Greg Grandin, THE BLOOD OF GUATEMALA: A HISTORY OF RACE AND NATION (Durham, N.C.: Duke University Press, 2000)

John L. Hammond, FIGHTING TO LEARN: POPULAR EDUCATION AND GUERRILLA WAR IN EL SALVADOR (New Brunswick, N.J.: Rutgers University Press, 1998)

César J. Ayala, AMERICAN SUGAR KINGDOM. THE PLANTATION ECONOMY OF THE SPANISH CARIBBEAN 1898–1934 (Chapel Hill: University of North Carolina Press, 1999)

Christopher David Brady, “Mid‐Century American Marxist: The Progessive Education of Leo Huberman.” M.A. Thesis in History, University of Oregon, Eugene, 1999; (for ordering information, call 800–521–3042 [order #1396671])