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81.
82.
Valente-Campos S Yonamine M de Moraes Moreau RL Silva OA 《Forensic science international》2006,159(2-3):218-222
The objective of the present work was to compare previously published methods and provide validation data to detect simultaneously cocaine (COC), benzoylecgonine (BE) and norcocaine (NCOC) in nail. Finger and toenail samples (5mg) were cut in very small pieces and submitted to an initial procedure for external decontamination. Methanol (3 ml) was used to release analytes from the matrix. A cleanup step was performed simultaneously by solid-phase extraction (SPE) and the residue was derivatized with pentafluoropropionic anhydride/pentafluoropropanol (PFPA/PFP). Gas chromatography-mass spectrometry (GC-MS) was used to detect the analytes in selected ion monitoring mode (SIM). Confidence parameters of validation of the method were: recovery, intra- and inter-assay precision, as well as limit of detection (LOD) of the analytes. The limits of detection were: 3.5 ng/mg for NCOC and 3.0 ng/mg for COC and BE. Good intra-assay precision was observed for all detected substances (coefficient of variation (CV)<11%). The inter-assay precision for norcocaine and benzoylecgonine were <4%. For intra- and inter-assay precision deuterated internal standards were used. Toenail and fingernail samples from eight declared cocaine users were submitted to the validated method. 相似文献
83.
Frigi S Pereira F Pereira L Yacoubi B Gusmão L Alves C Khodjet el Khil H Cherni L Amorim A El Gaaied A 《Forensic science international》2006,160(1):80-83
The 17 Y-chromosomal short tandem repeats (STRs) included in the AmpFLSTR Yfiler PCR Amplification Kit (AB Applied Biosystems) (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635 and GATA H4) were typed in two Berber communities, a small village (Takrouna) and a town (Sejenane), from North Tunisia. As expected, diversity was higher in the town, even when compared with a pool of three small Berber communities, probably due to the combination of different founder effects and genetic drifts operating in the small villages. 相似文献
84.
The Advent of Internet Surveys for Political Research: A Comparison of Telephone and Internet Samples 总被引:5,自引:0,他引:5
Berrens Robert P.; Bohara Alok K.; Jenkins-Smith Hank; Silva Carol; Weimer David L. 《Political Analysis》2003,11(1):1-22
The Internet offers a number of advantages as a survey mode:low marginal cost per completed response, capabilities for providingrespondents with large quantities of information, speed, andelimination of interviewer bias. Those seeking these advantagesconfront the problem of representativeness both in terms ofcoverage of the population and capabilities for drawing randomsamples. Two major strategies have been pursued commerciallyto develop the Internet as a survey mode. One strategy, usedby Harris Interactive, involves assembling a large panel ofwilling respondents who can be sampled. Another strategy, usedby Knowledge Networks, involves using random digit dialing (RDD)telephone methods to recruit households to a panel of Web-TVenabled respondents. Do these panels adequately deal with theproblem of representativeness to be useful in political scienceresearch? The authors address this question with results fromparallel surveys on global climate change and the Kyoto Protocoladministered by telephone to a national probability sample andby Internet to samples of the Harris Interactive and KnowledgeNetworks panels. Knowledge and opinion questions generally showstatistically significant but substantively modest differenceacross the modes. With inclusion of standard demographic controls,typical relational models of interest to political scientistsproduce similar estimates of parameters across modes. It thusappears that, with appropriate weighting, samples from thesepanels are sufficiently representative of the U.S. populationto be reasonable alternatives in many applications to samplesgathered through RDD telephone surveys. 相似文献
85.
Sánchez-Diz P Gusmão L Beleza S Benítez-Páez A Castro A García O Solla LP Geada H Martín P Martínez-Jarreta B de Fátima Pinheiro M Raimondi E Silva de la Fuente SM Vide MC Whittle MR Zarrabeitia MT Carracedo A Amorim A;GEP-ISFG 《Forensic science international》2003,135(2):158-162
A collaborative exercise was carried out by the Spanish and Portuguese ISFG Working Group (GEP-ISFG) in order to evaluate the performance of two Y-chromosome STR PCR tetraplexes, which include the loci DYS461, GATA C4, DYS437 and DYS438 (GEPY I), and DYS460, GATA A10, GATA H4 and DYS439 (GEPY II). The participating laboratories were asked to type three samples for the eight markers, using a specific amplification protocol. In addition, two control samples, with known haplotypes, were provided. The results obtained by the 13 different participating laboratories were identical, except for two laboratories that failed to type correctly the same two samples for GATA C4. By sequence analyses, two different GATA C4 allele structures were found. One control sample (allele 21) and two questioned samples (allele 22, correctly typed by all the laboratories, and allele 25) presented the following repeat structure: (TCTA)4(TGTA)2(TCTA)2(TGTA)2(TCTA)n, but different from the one found for allele 26 in one sample included in this exercise, as well as in the second control sample (allele 23), namely (TCTA)4(TGTA)2(TCTA)2(TGTA)2(TCTA)2(TGTA)2(TCTA)n. The collaborative exercise results proved that both Y-tetraplexes produce good amplification results, with the advantage of being efficiently typed using different separation and detection methodologies. However, since GATA C4 repeat presents a complex structure, with alleles differing in sequence structure, efficient denaturing conditions should be followed in order to avoid typing errors due to sizing problems. 相似文献
86.
Krous HF Nadeau JM Silva PD Blackbourne BD 《The American journal of forensic medicine and pathology》2003,24(1):1-8
Upper respiratory infection and pulmonary inflammation are common in sudden infant death syndrome, but their role in the cause of death remains controversial. Controlled studies comparing clinical upper respiratory infection and inflammation in sudden infant death syndrome with sudden infant deaths caused by accidents and inflicted injuries (controls) are unavailable. Our aim was to compare respiratory inflammation and upper respiratory infection within 48 hours of death and postmortem culture results in these two groups. A retrospective analysis of upper respiratory infection and pathologic variables in the trachea and lung of 155 infants dying of sudden infant death syndrome and 33 control infants was undertaken. Upper respiratory infection was present in 39% of sudden infant death syndrome cases and 40% of control cases. Upper respiratory infection was more likely to have occurred in association with more severe lymphocytic interstitial pneumonitis when sudden infant death syndrome cases and control cases were combined ( P=.04). Proximal and distal tracheal lymphocytic infiltration was more severe in control cases than in sudden infant death syndrome cases ( P=.01 and.01, respectively). Lymphocytic infiltrations of the bronchi, bronchioles, and pulmonary interstitium were similar between groups. Bronchial associated lymphoid tissue was more prominent in control cases ( P=.04). Cultures were positive in 80% of sudden infant death syndrome cases, 78% of which were polymicrobial. Among control cases, 89% were positive, with 94% being polymicrobial. This study confirms that microscopic inflammatory infiltrates in sudden infant death syndrome are not lethal. 相似文献
87.
Allele frequency data for the 15 STR systems and Amelogenine were determined in a population sample of healthy Mestizo unrelated individuals. All loci met Hardy-Weinberg expectations and the high discrimination power of combined system showed the forensic efficiency of these genetic markers. There is a lack of information on Ecuadorian population from a genetic point of view and therefore no previous publications on the distribution of STRs on Mestizos is available. Mestizos are descendents of Spanish and Amerindian people, however, significant differences were found between the former and the populations from Spanish peninsula, that have been analyzed for these genetic markers. 相似文献
88.
Bydlowski SP de Moura-Neto RS Soares RP Silva R Debes-Bravo AA Morganti L 《Forensic science international》2003,135(1):67-71
Allelic frequencies for 12 short tandem repeats (STRs) (F13A01, F13B, FESFPS, LPL, CSF1PO, TPOX, TH01, vWA, D16S539, D7S820, D13S317 and D5S818) were estimated, also as forensic parameters, from a sample of 916 unrelated Brazilian subjects classified into four ethnic groups: European-derived, African-derived, Brazilian Mulattos and Asian-derived. 相似文献
89.
Surprisingly, the association of neuroleptic-induced akathisia and aggressive behavior was not formally recognized until nearly two and one-half decades of antipsychotic prescribing had passed. Using a search of the anglophonic literature, this phenomenon is reviewed. Advances in psychopharmacology have reduced neuroleptic-induced akathisia and hold promise to eliminate it altogether. Nonetheless, important clinical and forensic aspects of neuroleptic-induced akathisia and aggression remain and are explored. 相似文献
90.
Silva MC 《Health matrix (Cleveland, Ohio : 1991)》1984,2(2):55-63
Using Levy's criteria of appraisal for a code of professional ethics, this article assesses the 1976 American Nurses' Association's Code for Nurses with Interpretive Statements in terms of purposes, specificity, inclusiveness, and enforceability. The assessment shows that the Code for Nurses meets generally accepted purposes for a code, contains enough specificity and inclusiveness to serve as a useful guide to professional conduct, and describes initial and follow-up action nurses can take when they witness questionable or inappropriate conduct that may adversely affect the patients' health care or safety. However, limited data are available regarding how the professional body has applied sanctions for violations of the Code. Further study on this matter is indicated and, fortunately, is forthcoming by ANA in an ongoing, planned survey. 相似文献