Effectiveness of Single‐Nucleotide Polymorphisms to Investigate Cattle Rustling

Short tandem repeats (STR)s have been the eligible markers for forensic animal genetics, despite single‐nucleotide polymorphisms (SNP)s became acceptable. The technology, the type, and amount of markers could limit the investigation in degraded forensic samples. The performance of a 32‐SNP panel genotyped through OpenArrays^TM (real‐time PCR based) was evaluated to resolve cattle‐specific forensic cases. DNA from different biological sources was used, including samples from an alleged instance of cattle rustling. SNPs and STRs performance and repeatability were compared. SNP call rate was variable among sample type (average = 80.18%), while forensic samples showed the lowest value (70.94%). The repeatability obtained (98.7%) supports the used technology. SNPs had better call rates than STRs in 12 of 20 casework samples, while forensic index values were similar for both panels. In conclusion, the 32‐SNPs used are as informative as the standard bovine STR battery and hence are suitable to resolve cattle rustling investigations.     

The talk of women friends

The talk of women friends has, for a variety of reasons, received little serious attention. We review (1) the sources of prejudice against this topic, (2) the dimensions of social context that shape women's friendships, and (3) the major differences between male friendships and female friendships. On the basis of prior research and an interview study, we propose that talk is central to close friendships between women. Our interview data reveal a broad range of conversational topics among women friends. The women we interviewed report that talk with their close friends creates a mosaic of noncritical listening, mutual support, enhancement of self-worth, relationship exclusiveness, and personal growth and self-discovery. We conclude by addressing issues pertaining to research methodology, to cross-cultural and sub-cultural differences, and to the politics of female friendship.     

Neo-liberalism,big business and the evolution of interest group activity in Latin America

In the 1980s and 1990s, the confluence of the third wave of democracy, neo-liberal economic reforms and economic crises in Latin America, produced several significant consequences for the region's underdeveloped interest group systems. By using an international political economy approach, this article examines these developments and particularly how neo-liberal policies affected the political fortunes of big business plus the broader political fall-out from neo-liberal policies. In essence, we make the argument that, for three reasons, the consequences of the confluence of these three developments for Latin America's emerging interest group system are mixed in terms of a more pluralist, open-access system. First, the influence of big business persists and in many ways has been enhanced as the economically and politically privileged position of large private companies since the 1980s has given way to economic concentration, transnationalization and the rise of multilatinas (Latin American multinational companies, which primarily operate across the region). Second, political opportunities have been opened for a range of interests, many from the left, that likely would not otherwise have emerged so early in the region. Third, the election of leaders opposed to neo-liberal policies may transform Latin America's political economy and aid in the democratization of its interest group system. Copyright © 2012 John Wiley & Sons, Ltd.     

Genetic polymorphisms at four STR loci from the HLA region in a Venezuelan population

POPULATIONS: Whole blood samples from 74 unrelated healthy individuals were collected. The donors' sample included Venezuelan mestizos from various regions of the country, but mostly from the resident population of Caracas City. A Venezuelan mestizo is the offspring of a mating between a native Venezuelan and a person born in Europe, mainly in Spain.     

Psychological treatment of men convicted of gender violence: a pilot study in Spanish prisons

In this article, the effectiveness of a psychological treatment program for men who are in prison for committing a serious offence of gender violence is tested. The sample consisted of 52 men who were imprisoned in eight Spanish prisons. The psychological treatment was a cognitive-behavioral program, in a group format, with 20 weekly sessions that lasted for 8 months. The results showed a significant improvement of irrational beliefs, about both women and violence, as a strategy to cope with everyday difficulties and a significant decrease of psychopathological symptomatology, anger, and hostility. On the other hand, results indicated that the only difference between the patients who dropped out of treatment and those who completed it was the level of initial motivation for treatment. Implications of this study for clinical practice and future research in this field are discussed.     

Aggressors against women in prison and in the community: an exploratory study of a differential profile

The current study compares the demographic and psychopathological characteristics of 54 men, who were in prison because of a serious offence of violence against women, and of 42 men, who belonged to a program of community treatment for violence against women in the home. There were no significant differences in demographic variables between the two samples. However, from a psychopathological point of view, psychiatric antecedents and current emotional instability were much more frequent and severe in aggressors within the community. Therefore, two possible differential profiles among the violent men are presented. Implications of these results for further research and clinical practice are commented on.     

Latex agglutination for bacterial antigens and meningococcus PCR: two useful tools in legal sudden deaths

Bacterial infections are considered to be a major cause of sudden deaths. The recognition of infections caused by Neisseria meningitidis is an essential duty of medicolegal offices due to the risk of secondary cases. Since other microorganisms, such as Haemophilus influenzae and Streptococcus pneumoniae, are also involved in infectious sudden deaths, the identification of the pathogen responsible for death is essential in order to establish a positive diagnosis while also preventing secondary meningococcal cases. However, because of the unreliability of culture methods used for autopsy specimens and the fragile nature of the microorganisms, other techniques were used. In this study, the detection of specific antigens of N. meningitidis (serogroups A, B, C, Y and W135), H. influenzae type b, S. pneumoniae and Group B Streptococcus was undertaken in 40 samples from sudden death cases in legal procedures with a latex agglutination test. In addition, a meningococcus polymerase chain reaction (PCR) assay (ctrA, crgA and siaD genes) was also used as a corroboration method for positive N. meningitidis agglutinations. Eleven cases of sudden death were confirmed to be due to meningococcus while one case was confirmed to have been caused by H. influenzae type b fulminant epiglottitis. Rapid laboratory diagnosis of meningococcal infection allowed contacts management and notification to the health authorities. From the point of view of the authors, forensic diagnosis of unascertained deaths should include latex agglutination and meningococcus PCR when a fulminant infection by N. meningitidis or H. influenzae is suspected as well as in deaths where the cause is unclear.     

Population frequency distribution of HumF13A01, HumFXIIIB, and HumLIPOL loci in the Basque Country (Northern Spain)

A population study of unrelated individuals from the Basque Country (Northern Spain) was carried out using the GenePrint STR System. The PCR products were separated on denaturing polyacrylamide gels and visualized by silver staining. Three tetrameric loci were evaluated: HumF13A01, HumFXIIIB, and HumLIPOL. All loci fit Hardy-Weinberg expectations, and independence of alleles was found between these STR loci. A comparison with other population groups indicated allele frequencies are well conserved in Caucasians, but differ from other racial groups. The calculated parameters a priori probability of exclusion (Pex) and "power of discrimination" (PD) show how informative these loci are for the determination of identity and relatedness of individuals.