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One of the most important longstanding problems in the field of forensic medicine is the determination of the time of death upon the discovery of a possible homicide victim. With a majority of homicide victims discovered within the first 48h, it is critically important to be able to determine time of death quickly, and with accuracy and precision. Current methods of determining postmortem interval (PMI) vary, but none can provide better than an 8-h window time estimate. In this paper, the potential application of single-cell gel electrophoresis (SCGE), also known as the comet assay, to evaluate postmortem cell death processes, specifically nuclear DNA fragmentation, is assessed. Upon the death of an organism, internal nucleases contained within the cells should cause chromosomal DNA to degrade into increasingly smaller fragments over time, and if these fragments can be isolated and visualized, the fragmentation should prove to be measurable and quantifiable. An original study providing proof of the concept of postmortem DNA fragmentation between early and late time periods was conducted using human leukocytes. With an established trend seen in the leukocyte results, this study was then expanded using a porcine animal model, over a longer time period, with more frequent time-points evaluated. DNA degradation in all samples was revealed by SCGE and quantified by the use of DNA-specific quantitative stains, as measured by digital camera affixed to a microscope. The comet 'tail-moment' gave a measure of the proportion of fragmented to non-fragmented DNA, while the 'tail-length' provided the relative size of degraded DNA fragments. In both models, an increase in DNA fragmentation was found to correlate with an increased PMI from 0 to 56h postmortem, as evaluated by comet-tail-moment and by comet-tail-length, with tail-length providing the strongest statistical correlation, based upon regression analysis. The postmortem DNA fragmentation observed in this study, reveals a sequential, time-dependent process with the potential for use as a predictor of PMI in homicide cases. 相似文献
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John Ferris 《Intelligence & National Security》2013,28(1):53-84
This essay examines the evidence and the literature on British signals intelligence between 1892 and 1945. It assesses the relative significance of the documents on signals intelligence released since the Waldegrave Initiative. It criticizes many conventional assumptions in the literature and argues that signals intelligence has been a normal practice of the British government throughout the twentieth century. The text sketches an alternative history of British signals intelligence during 1892–1945 and analyses its value for the British state in various aspects of the two world wars and diplomacy during the inter-war period. 相似文献
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In this paper we reexamine the apparently conflicting empirics of Borcherding et al. (1977) versus those of Barry and Lowery (1984). The latter, designed to test the cost disease versus bureau voting power hypotheses on US Citibase annual data between 1947 to 1979, was retested for the longer period available through 1989. Second, and more importantly, we isolate and test for the presence of a second channel for the exercise of bureaucratic power. That channel is the bureau's ability to use its information advantage to capture a portion of newly generated government rents through higher personal benefits (such as higher salaries). Such an analysis (following West, 1991) requires first that those factors generating new rents for government actually result in successful bureaucratic rent-seeking in the form of higher compensation levels. In addition, the analysis requires that these “artificial” increases in bureaucratic wages show up as significant determinants of the higher cost of providing government services. Incorporating a constructed Kau/Rubin variable into the Barry and Lowery database is then shown to improve the predictive power of both the cost disease and bureaucratic power hypotheses for US annual data between 1948 and 1989. 相似文献
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Determined to combat high levels of crime in two neighborhoods, a southern city implemented the Neighborhood Enhancement Team
(NET) to focus on gang and drug activity through a combination foot and bicycle patrol. With the support and assistance of
the community and its various agencies, the program's initiatives and outcomes have been successful. Compared to arrest rates
before the implementation of NET, the communities experienced declining arrest numbers far surpassing those of national trends:
The total number of arrests for these two communities over the first five years of NET implementation declined by 40%. Furthermore,
the annual arrest numbers are continuing their downward trend. Since the inception of the NET program, many youth are now
choosing alternatives to gang affiliations, and as a result, social order is returning to the communities. It is the purpose
of this article to delineate those initiatives and present findings of the outcomes.
Authors' Note: Ferris Byxbe, Ph.D., is Associate Professor, Director of Criminal Justice and Graduate Coordinator, in the Division of Social
Sciences, Criminal Justice Program, Delta State University, Cleveland, MS 38733. Philip Carlan, Ph.D., is Assistant Professor,
Department of Social Work & Criminal Justice, University of North Alabama, Florence. 相似文献
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John Ferris 《Intelligence & National Security》2013,28(5):675-696
The roles of intelligence, communications and signals in crisis decision-making routinely are mentioned in passing but rarely assessed in detail. This study examines these issues in three international crises: the great eastern question, 1877–78, Chanak, 1922 and Munich, 1938, and briefly compares these findings to two others, July 1914 and Cuba, 1962. It demonstrates that intelligence, communications and signals are more problematical in crises than is generally believed. This study challenges the conventional view that crises are essentially something to manage. Instead, it argues, crises are explosive, unpredictable and high in risk, dominated by emotion, factionalization, communication failures, missed signals and unintended consequences. 相似文献
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