Single cells for forensic DNA analysis--from evidence material to test tube

Abstract: The purpose of this project was to develop a method that, while providing morphological quality control, allows single cells to be obtained from the surfaces of various evidence materials and be made available for DNA analysis in cases where only small amounts of cell material are present or where only mixed traces are found. With the SteREO Lumar.V12 stereomicroscope and UV unit from Zeiss, it was possible to detect and assess single epithelial cells on the surfaces of various objects (e.g., glass, plastic, metal). A digitally operated micromanipulator developed by aura optik was used to lift a single cell from the surface of evidence material and to transfer it to a conventional PCR tube or to an AmpliGrid^® from Advalytix. The actual lifting of the cells was performed with microglobes that acted as carriers. The microglobes were held with microtweezers and were transferred to the DNA analysis receptacles along with the adhering cells. In a next step, the PCR can be carried out in this receptacle without removing the microglobe. Our method allows a single cell to be isolated directly from evidence material and be made available for forensic DNA analysis.     

The effects of corrosive substances on human bone, teeth, hair, nails, and soft tissue

Abstract: This research investigates the effects of household chemicals on human tissues. Five different human tissues (bone, tooth, hair, fingernails, and skin/muscle/fat) were immersed into six different corrosive agents. These agents consisted of hydrochloric acid, sulfuric acid, lye, bleach, organic septic cleaner, and Coca‐Cola^® soda. Tap water was used as a control. Tissue samples were cut to consistent sizes and submerged in the corrosive liquids. Over time, the appearance, consistency, and weight were documented. Hydrochloric acid was the most destructive agent in this study, consuming most tissues within 24 h. Sulfuric acid was the second most destructive agent in this study. Bleach, lye, and cola had no structural effects on the hard tissues of the body, but did alter the appearance or integrity of the hair, nails, or flesh in some way. The organic septic cleaner and tap water had no effect on any of the human tissue tested during the timeframe of the study.     

Reverse engineering--rapid prototyping of the skull in forensic trauma analysis

Rapid prototyping (RP) comprises a variety of automated manufacturing techniques such as selective laser sintering (SLS), stereolithography, and three-dimensional printing (3DP), which use virtual 3D data sets to fabricate solid forms in a layer-by-layer technique. Despite a growing demand for (virtual) reconstruction models in daily forensic casework, maceration of the skull is frequently assigned to ensure haptic evidence presentation in the courtroom. Owing to the progress in the field of forensic radiology, 3D data sets of relevant cases are usually available to the forensic expert. Here, we present a first application of RP in forensic medicine using computed tomography scans for the fabrication of an SLS skull model in a case of fatal hammer impacts to the head. The report is intended to show that this method fully respects the dignity of the deceased and is consistent with medical ethics but nevertheless provides an excellent 3D impression of anatomical structures and injuries.     

“He did everything he possibly could for me”: medical malpractice claimants’ experiences of lawyer–client relations

Lawyers are obliged to act in the interests of their clients, however, it is not clear how lawyers should do this in practice. Should lawyers follow their clients' instructions without deviation, should they actively manage their clients' expectations, or as many studies suggest, do lawyers place their own interests first? This paper examines how lawyers interact with medical malpractice claimants. It reveals that lawyers take a client-aligned approach, where lawyers acknowledge their client's goals, but do not necessarily do what the client says. We argue that this approach is made necessary by legal and organisational constraints which limit the ability of lawyers to produce the types of outcomes that their clients want.     

The Impact of Exchange Rate on the Growth of Nigerian Economy From 1960 to 2010

This paper examined the impact of exchange rate on the growth of Nigerian economy using time series data from 1960 to 2010. The paper utilized secondary data and explored various econometrics and/or statistical analytical （E-view 7.0） method to examine the relationship between exchange rate and economic growth. The paper tested the stationarity--through unit root test （ADF）, Vector Autoregressive Estimates （VARs）, cointegration test, Granger-Causality test of Nigeria＇s time series data, and used an error correction model through over-parameterization and parsimonious model to determine the long-run relationship among the variables examined. It was discovered from the findings that the growth rate of national income was directly related to domestic investment and economic growth. The paper recommended based on the econometric results among others that the government should ensure that prevailing economic situation in Nigeria is tolerable in order to reap the full benefit/advantage of exchange rate policy, encourage domestic production of goods and services to enable the domestic economy to benefit from the exchange rate policy, ensure the stability of macroeconomics variables, and ensure the exchange rate policy which is to encourage exports and discourage imports in order to avoid cash drain （or flight） in the economy.     

The Evolving “Nature” of Environmental Risk: A Responsible Approach for Residential and Commercial Real Estate

Environmental losses suffered by commercial and residential real estate owners are becoming more frequent and severe due to evolving regulatory regimes and the changing global climate. This article reviews the nature of environmental risk, specifically within the context of a changing climate, and proposes the large-scale installation of green infrastructure as both a business opportunity for insurers and a responsible approach.     

The use of a 3-D laser scanner to document ephemeral evidence at crime scenes and postmortem examinations

Proper documentation of physical evidence at both crimes scenes and postmortem examination is crucial for downstream analysis, interpretation, and presentation in court. Ephemeral or transient evidence poses particular challenges to investigators, as its very nature renders it difficult or impossible to seize and maintain in its original physical state. The use of a hand-held three-dimensional (3-D) laser scanner is proposed to capture and document such evidence, both in the field and at autopsy. Advantages of the scanner over traditional means of documentation such as photography or casting include the ability to obtain measurements in all dimensions, the ability to reconstruct missing elements, and the ease with which generated images can be interpreted by the jury at trial. Potential scenarios warranting the use of the scanner are identified, and the limitations of its use are discussed.     

Driving under the influence of amphetamine-like drugs

Scientific opinions differ whether the use of stimulants causes deterioration in driving skills. In 1857 of 8709 cases of driving under the influence of drugs, amphetamine-like drugs (amphetamine, methamphetamine, and methylendioxyamphetamine) were present either alone or together with other licit or illicit drugs. In 338 cases, amphetamines were the only psychoactive substance group in plasma at mean, median, and highest concentrations of 0.18, 0.12, and 1.05 mg/L, respectively. A widespread opinion is that after the consumption of amphetamines, centrally stimulating effects with corresponding consequences on safe driving are expected. In contrast, many cases were observed that rather suggested an influence of centrally sedating substances when considering the psycho-physical conditions. Relations between concentration and effect could not be established. The apparent sedation is probably the consequence of sleep deprivation during an amphetamine binge and the after-effects of the drug.     

A simple method of nose tip shape validation for facial approximation

Despite numerous papers relating to the prediction of nose projection for the purposes of facial approximation, there is little guidance for nose tip shape that has been evaluated on a known data set. This study presents a novel, simple technique for validation of the reconstructed nose tip shape based on methods used in actual approximation practice. The data set was comprised of 25 full-head computed tomography (CT) patient scans. In 22 of the 25 patients across all age and sex groups, when the head is tilted so that soft tissue pronasale is superimposed on hard tissue rhinion, the curvature of the nose tip was found to mimic the curvature of the superior portion of the nasal aperture. This occurs when the head is tilted dorsally by approximately 60° (55.87±5.91). Individuals with snub noses presented a much wider tip curvature. The method was highly repeatable and was evaluated through inter- and intra-observer studies (error=3.15%).