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261.
C. Tomàs J.J. Sanchez A. Barbaro A. Hernandez M.M. Ramon 《Forensic Science International: Genetics Supplement Series》2008,1(1):170-172
The X-chromosome has valuable characteristics for population genetic studies. In order to investigate the genetics of the human Mediterranean populations further, we developed a 25 X-chromosome SNP-multiplex typing system. The system was based on PCR multiplex amplification and subsequent multiplex single base extension with the SNaPshot reaction, capillary electrophoresis and multicolor fluorescence detection.We investigated 11 Mediterranean populations with the 25 X-chromosome SNPs. A high overall homogeneity was found among the Mediterranean populations except for Moroccans, who differed genetically from the rest of the populations in the Mediterranean area. This result supports the hypothesis of a low incidence of the south-north genetic interchange at the western shores of the Mediterranean basin. A low genetic distance was found between populations in the Middle East and the western part of the Mediterranean area most likely reflecting the strong effect of the Neolithic wave.A certain level of background linkage disequilibrium among the 25 SNPs on the X-chromosome in Ibiza and Cosenza was observed, possibly as a consequence of their demographic history. 相似文献
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Christopher Phillips Anna Barbaro Luís Fernandez Formoso David Ballard Denise Syndercombe Court Ángel Carracedo Maviky Lareu 《Forensic Science International: Genetics Supplement Series》2009,2(1):25-26
We constructed a simple STR pentaplex of new loci recommended as next generation markers for the European Standard Set (ESS) comprising normal-amplicon STRs: D12S391 and D1S1656, plus mini-amplicon STRs: D2S441, D10S1248 and D22S1045. Validation of the pentaplex included evaluation of its ability to amplify DNA from a variety of degraded forensic casework samples. Although the ESS-pentaplex was designed in the first instance to generate allele frequency data to supplement existing databases of established STRs, the multiplex proved to be a valuable tool for the analysis of challenging DNA when certain markers of Identifiler or MiniFiler occasionally failed. 相似文献
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Stephen L. Gould Alan A. Oldaïl Fred Thompson 《Canadian public administration. Administration publique du Canada》1979,22(2):251-260
Abstract. This paper describes the consequences of an experiment conducted with four governmental programs intended to evaluate the utility of ZBB for improving public sector decision-making. The results of this experiment were mixed. On the one hand, we clearly observed greater participation by operating managers in the formulation of budgets. This, we were frequently told, had positive consequences for staff development and program planning. The zbb experiment also resulted in the generation of more and better information in support of budget requests. However, information has value only to the extent that it increases the probability that decision makers will choose better options than they would without it. In no case was it possible to identify changes in budget amounts or program content that might not have occurred following standard budgeting practices. We do not believe the absence of fiscal or program impact resulting from this experience is in any way conclusive evidence against zbb , but it should serve as a warning to the unwary or over-enthusiastic user. Sommaire. Cet article fait état d'une expérience conduite sur quatre programmes gouvernementaux dans le but d'évaluer l'utilité de la méthode zbb comme moyen d'améliorer le processus de décision à l'intérieur du secteur public. Les résultats de l'expérience sont partagés. D'une part, il apparaît évident que cette méthode suscite plus de participation chez les gestionnaires dans la formulation des budgets; cette participation accrue produit — nous a-t-on affirméà plusieurs reprises — des effets positifs sur le perfectionnement du personnel et sur la planification des programmes. L'expérience a eu aussi pour effet d'engendrer des informations plus nombreuses et de meilleure qualité aux fins de justification des demands budgétaires. Cependant, l'information n'a de valeur que dans la mesure où elle augmente la probabilité que de meilleurs choix seront effectués. Or, dans aucun cas, il n'a été possible d'identifier des changements dans les budgets alloués ou dans le contenu des programmes qui ne seraient pas survenus avec l'emploi des méthodes traditionnelles de préparation des budgets. Nous ne croyons pas que cette absence d'impact sur la fiscalité ou sur les programmes témoigne de façon concluante contre la méthode du zbb , mais c'est une mise en garde pour ceux qui seraient tentés de surestimer les avantages de cette méthode. 相似文献
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Presciuttini S Cerri N Turrina S Pennato B Alù M Asmundo A Barbaro A Boschi I Buscemi L Caenazzo L Carnevali E De Leo D Di Nunno C Domenici R Maniscalco M Peloso G Pelotti S Piccinini A Podini D Ricci U Robino C Saravo L Verzeletti A Venturi M Tagliabracci A 《Forensic science international》2006,156(2-3):266-268
Results from a collaborative exercise with proficiency testing conducted by 20 Italian laboratories on the 15 loci included in the Identifiler kit were analyzed by allele sharing methods and by standard population genetics tests. The validated database, including about 1500 subjects, was merged with that of a previous exercise conducted on nine loci, and the resulting allele frequencies, subdivided by Italian region, were published on-line. 相似文献
270.
Robert M. Malow Jessy G. Dévieux Ligia Martinez Fred Peipman Barbara A. Lucenko Seth C. Kalichman 《Journal of family violence》2006,21(2):127-135
Epidemiological studies in the United States estimate HIV seroprevalence rates ranging between 4% and 23% for serious mentally ill adults (SMIA; i.e., schizophrenia, bipolar disorder, and other chronic disorders with psychotic features), with substantially greater estimates for risky sexual behaviors. Among the most consistent factors linked to HIV risk in non-SMIA populations is a history of emotional, physical, or sexual abuse. The current study examined the intersection of abuse history and HIV sexual risk behavior in SMIAs with a group of men (n = 64) and women (n = 115) recruited from eight geographically diverse and representative clinical sites around Florida. A cross-sectional interview revealed a high prevalence of alcohol and other drugs problems, as well as a history of emotional, physical, or sexual abuse in 75% of the participants, with 68% of these reporting multiple types of abuse. Compared to nonabused counterparts, those with a history of abuse reported significantly greater: (a) lifetime and current psychiatric symptoms, (b) recent unprotected sexual intercourse, and (c) crack cocaine, heroin, and marijuana use. No gender differences were detected in the associations of abuse history and sexual risk behaviors. These results underscore the need for HIV prevention efforts targeted to SMIAs and the critical importance of addressing abuse history in interventions with this underserved population. 相似文献