Quantitation of human genomic DNA through amplification of the amelogenin locus

An alternate method for quantitation of human genomic DNA is presented. Quantitative template amplification technology (abbreviated "Q-TAT") estimates the quantity of human DNA present in an extract by comparing fluorescence in X and Y amplicons produced from unknowns with fluorescence in a standard curve amplified from known quantities of reference DNA. Q-TAT utilizes PCR and electrophoresis with fluorescent detection/quantitation, precluding the need for new instrumentation, methodology, or quality assurance associated with slot-blot or real-time PCR. In a comparison study incorporating shared samples, Q-TAT was found to be more sensitive than widely used slot-blot methods but somewhat less sensitive than real-time PCR. Among samples containing DNA concentrations ranging from 100 pg/microL to 2-4 ng/microL, Q-TAT produced DNA concentration estimates that agreed reasonably well with either Quantiblot or real-time PCR. Q-TAT was reproducible with a typical coincidence of variation of about 35%. Quantitation of human DNA in this study involved summing fluorescence in X and Y amplicons in unknowns and quantitation standards. However, analyzing fluorescence in X and Y amplicons individually could allow estimates of male and female DNA present in mixtures to be made. Moreover, since X and Y amplicons exhibit sizes of 210 and 216 bp, respectively, the integrity as well as the concentration of the genomic DNA template can be assessed. Q-TAT represents an alternate method useful for the quantitation of human genomic DNA prior to amplification of STR loci used for identity testing purposes. The method uses existing equipment and procedures in conjunction with a well-characterized DNA standard to produce concentration estimates for unknowns that reliably produce STR profiles suitable for analysis.     

Theoretical and Methodological Challenges of Studying Refugee Children in the Middle East and North Africa: Young Palestinian, Afghan and Sahrawi Refugees

This article aims to address a number of conceptual and methodologicalchallenges facing the study of young refugees. Much of the researchon refugees has, until recently, been focused on adults, andto a lesser degree, on young children. Those studies that doinclude children are largely carried out in the domain of psychologyand psychiatry and tend to pathologize and individualize. Thisarticle is based on observations derived from a six-year, multi-disciplinaryanthropological and participatory research programme that examinedthe impact of forced migration on young people in the MiddleEast and North Africa: Palestinian refugee youth in Lebanon,Syria, Jordan, the West Bank and Gaza, Sahrawi youth in Algeria,and Afghan youth in Iran. It argues that despite the challenges,an anthropological and participatory approach contributes toa greater, more holistic understanding of refugee youth.     

The EDNAP mitochondrial DNA population database (EMPOP) collaborative exercises: organisation, results and perspectives

This paper presents an overview of the organisation and the results of the collaborative exercises (CE) of the European DNA Profiling (EDNAP) Group's mitochondrial DNA population database project (EMPOP). The aim of the collaborative exercises was to determine whether uniformity of mtDNA sequencing results could be achieved among different laboratories. These were asked to sequence either the complete mtDNA control region or the two hypervariable regions HVI (16024-16365) and HVII (73-340) from DNA extracts, buccal swabs or bloodstains, proceeding in accordance with the protocol and strategies used in each individual laboratory. The results of the collaborative exercises were employed to identify possible sources of errors that could arise during the analysis and interpretation of mtDNA profiles. These findings were taken as a basis to tentatively make suitable arrangements for the construction of a high quality mtDNA database. One hundred fifty mtDNA profiles were submitted to the evaluating laboratory, and disaccording profiles were classified into four groups corresponding to the source of error: clerical errors, sample mix-ups, contaminations and discrepancies with respect to the mtDNA nomenclature. Overall, 14 disaccording haplotypes (16 individual errors) were observed. The errors included 10 clerical errors, 3 interpretation problems, 2 cases of sample mix-up and 1 case of point heteroplasmic mixture, where the 2 sequencing reactions brought inconsistent base calls. This corresponds to an error rate of 10.7% in a virtual mtDNA database consisting of the collaborative exercise results. However, this estimate is still conservative compared to conclusions drawn by authors of meanwhile numerous publications critically reviewing published mtDNA population databases. Our results and earlier published concerns strongly emphasize the need for appropriate safety regulations when mtDNA profiles are compiled for database purposes in order to accomplish the high standard required for mtDNA databases that are used in the forensic context.     

Y-STR haplotypes from eight south Indian groups based on five loci

Five Y-chromosome short tandem repeat (STR) loci (DYS389I, DYS389II, DYS390, DYS391, and DYS393) were typed in 81 males from seven tribal populations of south India, including 30 Koragas, 23 Yeravas, 5 Mullukurunan, 2 Mullukurumba, 4 Paniya, 3 Kuruchian and 3 Bettakurumba; and 11 south Indian caste individuals of mixed origins.     

The Changing Limits and the Limits of Change: the state,private firms,international industry and China in the evolution of Taiwan's electronics industry

This paper examines the changing role of the state and private enterprise in Taiwan's electronics industry as the industry responds to growth of intra-firm capabilities and the rise of China as a manufacturing base. The industry has a sharp dichotomous outcome between the technologically strong IC sector, which has been able to create interdependence with global lead firms, and the systems manufacturing firms, which find themselves trapped in a dependent position vis-à-vis the global lead firms. Due to different positions in global value chains, the IC industry firms have been better able to create advantages out of the opportunities of new firm capabilities and China than the systems firms even as the balance of policy has shifted somewhat away from the state and towards private enterprise.     

The differentiation of toners used in photocopy processes by infrared spectroscopy

Toners (inks) used in plain paper photocopying machines may be discriminated by infrared spectroscopy using small samples of solid toner removed from the paper surface.     

Navigating the minefield: legal ethics and healthcare law

Practicing law in the healthcare field is a daunting task due to the highly-regulated nature of the field and the increasing scrutiny of the conduct of industry providers, payors, and vendors. Attorneys must provide difficult opinions regarding matters with civil, criminal, and reimbursement implications and often are asked to represent multiple parties in healthcare-related settings. This article discusses some legal ethics issues for the healthcare practitioner and touches on some of the recent changes to the Model Rules of Professional Conduct, which were adopted by the American Bar Association's House of Delegates at its mid-February 2002 meeting. The authors conduct their analysis by applying the model rules to a number of hypothetical fact situations typical of those encountered in the day-to-day practice of healthcare law.