The propensity of individuals to deposit DNA and secondary transfer of low level DNA from individuals to inert surfaces

We have shown that there is a difference between individuals in their tendency to deposit DNA on an item when it is touched. While a good DNA shedder may leave behind a full DNA profile immediately after hand washing, poor DNA shedders may only do so when their hands have not been washed for a period of 6h. We have also demonstrated that transfer of DNA from one individual (A) to another (B) and subsequently to an object is possible under specific laboratory conditions using the AMPFISTR SGM Plus multiplex at both 28 and 34 PCR cycles. This is a form of secondary transfer. If a 30 min or 1h delay was introduced before contact of individual B with the object then at 34 cycles a mixture of profiles from both individuals was recovered. We have also determined that the quantity and quality of DNA profiles recovered is dependent upon the particular individuals involved in the transfer process. The findings reported here are preliminary and further investigations are underway in order to further add to understanding of the issues of DNA transfer and persistence.     

The Paung Ku model: encouraging change through learning

It is well known within international development practice that the terms “capacity building” or “capacity development” are often used but infrequently (and inconsistently) defined; whether in funding applications, program strategies, staff training programmes, or field work. This article outlines the way in which one development organisation working in Burma/Myanmar wrestled with the issue of meaning, and practice, in relation to capacity development; it also reports on the resulting “Paung Ku model: encouraging change through learning.”