Evaluating Bioinformatic Pipeline Performance for Forensic Microbiome Analysis*,†,‡

Microbial communities have potential evidential utility for forensic applications. However, bioinformatic analysis of high-throughput sequencing data varies widely among laboratories. These differences can potentially affect microbial community composition and downstream analyses. To illustrate the importance of standardizing methodology, we compared analyses of postmortem microbiome samples using several bioinformatic pipelines, varying minimum library size or minimum number of sequences per sample, and sample size. Using the same input sequence data, we found that three open-source bioinformatic pipelines, MG-RAST, mothur, and QIIME2, had significant differences in relative abundance, alpha-diversity, and beta-diversity, despite the same input data. Increasing minimum library size and sample size increased the number of low-abundant and infrequent taxa detected. Our results show that bioinformatic pipeline and parameter choice affect results in important ways. Given the growing potential application of forensic microbiology to the criminal justice system, continued research on standardizing computational methodology will be important for downstream applications.     

The promises and pitfalls of polysemic ideas: ‘One Health’ and antimicrobial resistance policy in Australia and the UK

Policy Sciences - Recent scholarship posits that ambiguous (‘polysemic’) ideas are effective for coalition building between diverse stakeholders: their capacity to be interpreted...     

Chinese migrant women and families in Britain

This article examines the mode of understanding and experiences of family relationships of Chinese migrant women in Britain. In contrast to much existing research work on the patterns and experiences of postwar settlement of unskilled Chinese male labourers in Britain, the focus here is on the life stories of 41 Chinese women with different migration trajectories and varying economic and cultural capital. Their oral testimonies reveal Chinese women's diverse expectations and experiences of migrant family relationships and their different strategies to achieve self-fulfillment both within and outside the confines of the migrant family. For some women, migration brings opportunities for a fulfilling and independent lifestyle. They are successful in negotiating their way around and sometimes out of their initial familial and social position. For others, they bear the disproportionate cost and labour of familial strategies of advancement and remain vulnerable to the most constraining aspects of diasporic existence.     

Fatal accident with a special automobile

The authors report on a traffic accident in which the passenger of a Light Four-wheeled Vehicle (Microcar) approved for use by a disabled person was killed after a rear collision with a compact car. The severity of the accident trauma was critically determined by the vehicle's light construction. Consideration is also given to the legal situation regarding registration and driver's license issues.     

Social policy in rich and poor countries

Nomination: Does politics matter? A perennial question! by Hans Keman, p.159
Reflections: Politics does indeed matter! by Manfred G. Schmidt, p.164     

An unusual case of self-harm with petroleum distillate

After surgical treatment of an inguinal hernia, a 26-year-old male was discharged from hospital with a well healing surgical wound. Four weeks later he was admitted again with a major localized painful swelling of the soft tissues, whereas the clinical examination revealed no further local or systemic signs of inflammation. The immediate operation disclosed no abscess formation, but a large colliquation necrosis of the subcutaneous tissue with an outstanding petroleum-like odour. On toxicological examination of the necrotic tissue, a respective petroleum distillate was found. The patient was confronted with the suspicion of having injected the petroleum preparation himself to prolong the wound healing and finally admitted the self-mutilating behaviour. The pathophysiology, clinical history and morphological findings are discussed in comparison with case reports of chemically induced self-mutilation.     

Obtaining fingerprints from mummified fingers: a method for tissue rehydration adapted from the archeological literature

Our laboratory was asked to help with the rehydration of mummified human fingertips that had been removed from a recently deceased, unidentified female. Using a solution that was found in the archeological literature, we were able to successfully rehydrate dermal tissues to the extent that fingerprints could be taken. We believe that this solution, which until now has not been described in the forensic literature, is effective, affordable, and relatively easy to produce and use.