Cell line DNA typing in forensic genetics--the necessity of reliable standards

The incorporation of reference DNA is crucial to the validation of any DNA typing protocol. This paper aims to provide a panel of reference DNAs for actual forensic profiling strategies, i.e. autosomal and gonosomal STR typing as well as mtDNA sequencing. We have characterised three human lymphoid cell lines, GM9947, GM9948 and GM3657, and considered 58 autosomal and gonosomal microsatellites as well as the mitochondrial control region sequence. Well-established markers and STRs recently developed for forensic use were involved. K562 DNA samples which we purchased from two different suppliers were also analysed. They revealed conflicting results with regard to the ChrX STR marker genotype. Hence, we suggest that K562 is no longer used for the calibration of profiling techniques. Our investigation establishes a panel of one female and two male DNA samples as an STR allelic ladder calibration tool and offers information on six alleles of each autosome (AS) marker, three alleles of each X chromosome (ChrX) marker and two alleles of each ChrY marker. In addition, sequences of the mitochondrial control region of the three DNAs are communicated in order to provide sequencing quality control.     

Mitochondrial diversity of a northeast German population sample

Mitochondrial DNA sequences of the hypervariable regions HV I and HV II were analyzed in 300 unrelated individuals born and living in the northeast corner of Germany (Western Pomerania) to generate a database for forensic identification purposes in this region. Sequence polymorphism were detected using PCR and direct sequencing analysis. A total of 242 different haplotypes were found as determined by 147 variable positions. The most frequent haplotype (263G, 315.1C) was found in 10 individuals and is also the most common sequence in Europe. Three other haplotypes were shared by 5 individuals, 2 sequences by 4, 8 haplotypes by 3, 15 sequences by 2 persons, and 213 sequences were unique. The genetic diversity was estimated to be 0.99 and the probability of two random individuals showing identical mitochondrial DNA (mtDNA) haplotypes is 0.6%. A comparison with other studies from Germany showed only little differences in the distribution of haplogroups. Nevertheless, one frequent haplotype in northeast Germany (five unrelated individuals) could only rarely be found in other German and European regions. Our results may indicate that despite a high admixture proportion in the German population some regions could demonstrate certain characteristic features.     

A novel platform for the modular integration of forensic assay setup and medium- to high-throughput purification of nucleic acids

With its extraction and assay setup modules, the QIAsymphony^® provides a highly flexible solution for processing forensic samples in a medium- to high-throughput scale. We tested the sensitivity of extraction, precision of sample processing, and accuracy of automated assay setup of this integrated system. Results attest to QIAsymphony's ability to isolate DNA from a spectrum of common forensic samples and process these samples without cross-contamination. Furthermore, accurate assay setup for downstream applications, like PCR, make this system highly suited for enhancing laboratory workflow.     

“The Good,the Bad and the Ugly” Reconsidered: A Comparison of German,Canadian and Spanish Labour Migration Policies

This article compares three immigration countries that are perceived, both publicly and politically, as being fundamentally different: Canada, which is allegedly one of the most attractive destination countries for labour migrants worldwide; Germany, which is still thought to be sceptical towards immigration; and finally Spain, which is considered institutionally incapable of implementing efficient labour migration policies. Against the backdrop of various political reforms that have been adopted in these countries, however, the article argues that such a distinction is becoming increasingly inaccurate. This observation is backed empirically by analysing the technique of screening labour migrants and the temporal design of labour migration policy.     

The Interaction Effects of Bargaining Power: The Interplay Between Veto Power,Asymmetric Interdependence,Reputation, and Audience Costs

In international relations, different rationalistic theories have developed to explain negotiators’ behavior and the outcomes of negotiations. The compatibility and interaction effects between the different forms of bargaining power, however, remain unexplored. In this article, I seek to fill this gap by connecting four rationalistic concepts of bargaining power: veto power, asymmetric interdependence, reputation, and audience costs. By showing that domestic veto players are only semiveto players in international politics – because they can veto an improvement but not a deterioration of the status quo – threats based on asymmetric interdependence to disrupt a mutually beneficial cooperative relationship can be connected to veto power; the incompatibility of the factors concerned would otherwise make this impossible. The combination of veto power and asymmetric interdependence, however, raises a theoretical question: Will rational actors ever approve a deterioration of the status quo? Theories of reputation and audience costs can help answer this question. According to these approaches, threatening parties suffer ex post costs when they back down from their own threats. This theoretical analysis sheds new light on how different forms of bargaining power interact with each other and also helps to address some of the theoretical inconsistencies of the original individual concepts. Finally, this analysis suggests some of the weaknesses of empirical studies that have neglected these interaction effects.     

FDI Liberalisation,Firm Heterogeneity and Foreign Ownership: German Firm Decisions in Reforming India

The paper investigates the role of firm-level productivity and industry-level R&D for multinational enterprises' (MNEs') choice of undertaking foreign direct investment (FDI), and the share of ownership in foreign affiliates. Two firm-specific datasets on German MNEs with varying equity stakes in Indian affiliates are used to account for the two-step decision process. The paper also analyses how German firm decisions were affected by the liberalisation of FDI regulations in India. Results show remarkable differences between the selection and the ownership share equation, and also between the pre-reform and post-reform periods. The evidence clearly reveals the trade-offs involved in selective FDI approvals and foreign ownership restrictions.     

Trust in Politicians and Satisfaction with Government – A Reciprocal Causation Approach for European Countries

Trust and satisfaction with the government are the most important indicators of a functioning democratic system. Scholars diverge in their opinions of whether satisfaction causes trust or vice versa or whether both variables reinforce each other simultaneously. This study applies an instrumental variable approach to shed light on this discussion. We employ a sample of 7166 individuals in 3 European countries (i.e., Germany, France, and Great Britain) obtained from the European Social Survey to test this reciprocal relationship. By comparing these three countries with fundamentally different political systems, we investigate the contingency of the reciprocal effect with regard to the political context. The results confirm the reciprocal relationship but reveal differences between the three investigated countries.     

Soziale Gerechtigkeit und der Wirkungsgrad der Mitbestimmung

Der Beitrag diskutiert die Frage, warum Betriebsräte in Unternehmen mit ähnlicher rechtlicher, ökonomischer oder organisatorischer Struktur in jeweils unterschiedlichem Ausmaß an den Entscheidungen ihrer Geschäftsleitungen beteiligt sind. Im Anschluss an eine kritische Diskussion der motivationalen Basisannahmen des Mitbestimmungshandelns wird behauptet, dass Gerechtigkeitseinstellungen zur Formierung und zur Legitimation von Interessen gegenüber der Verteilung von Gütern und Lasten im Unternehmen beitragen. Je nachdem, in welcher Konstellation ergebnis- und ordnungsbezogene Gerechtigkeitseinstellungen von einer Belegschaft vertreten werden, ergibt sich eine andere Machtposition des Betriebsrats und somit ein anderer Wirkungsgrad der Mitbestimmung im jeweiligen Unternehmen. Inwiefern die postulierten Zusammenhänge empirisch Bestand haben, wird unter Rückgriff auf Daten aus einer Beschäftigtenbefragung untersucht, die 1999 in 21 Unternehmen der deutschen Metallindustrie durchgeführt wurde.     

Does Coup-Proofing Work? Political–Military Relations in Authoritarian Regimes amid the Arab Uprisings

The popular mass uprisings in the Middle East and North Africa (MENA) call into question the assumption, widespread prior to the “Arab Spring”, that militaries in these countries were subservient to civilianized and consolidated authoritarian regime incumbents. In most countries militaries have stepped in to suppress uprisings, replace incumbents, or cause civil wars. The analysis of political-military relations explains the immediate outcome of popular mass mobilization in the MENA region and helps re-conceptualize coup-proofing as an important authoritarian survival strategy. Accounting for variation in the degree of officers' loyalty toward incumbents provides an opportunity to test the efficacy of coup-proofing. The article accounts for questions largely ignored in the theoretical literature: which coup-proofing mechanisms work best, and under which circumstances? In a qualitative comparison of Egypt and Syria, the article illustrates that authoritarian regimes have applied fundamentally different coup-proofing strategies. The Syrian regime has engineered integrative strategies to tie officers closer to the incumbent, provoking a greater degree of loyalty during regime crisis than in Egypt where officers were excluded from politics.