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91.
Detection and quantification of the age-related point mutation A189G in the human mitochondrial DNA 总被引:2,自引:0,他引:2
Thèves C Keyser-Tracqui C Crubézy E Salles JP Ludes B Telmon N 《Journal of forensic sciences》2006,51(4):865-873
Mutation analysis in the mitochondrial DNA (mtDNA) control region is widely used in population genetic studies as well as in forensic medicine. Among the difficulties linked to the mtDNA analysis, one can find the detection of heteroplasmy, which can be inherited or somatic. Recently, age-related point mutation A189G was described in mtDNA and shown to accumulate with age in muscles. We carried out the detection of this 189 heteroplasmic point mutation using three technologies: automated DNA sequencing, Southern blot hybridization using a digoxigenin-labeled oligonucleotide probe, and peptide nucleic acid (PNA)/real-time PCR combined method on different biological samples. Our results give additional information on the increase in mutation frequency with age in muscle tissue and revealed that the PNA/real-time PCR is a largely more sensitive method than DNA sequencing for heteroplasmy detection. These investigations could be of interest in the detection and interpretation of mtDNA heteroplasmy in anthropological and forensic studies. 相似文献
92.
The use of a drug to modify a person's behaviour for criminal gain is not a recent phenomenon. However, the recent increase in reports of drug-facilitated crimes (sexual assault, robbery) has caused alarm by the general public. Among the drugs that can be used, alprazolam (Xanax), an anxiolytic benzodiazepine, has been seldom observed. To document two cases involving this drug, we have developed an approach based on hair testing by LC-MS/MS. After pH 8.4 buffer incubation and extraction with methylene chloride/diethyl ether (80/20, v/v), hair extracts were separated on a XTerra MS C18 column using a gradient of acetonitrile and formate buffer. Alprazolam and diazepam-d5, used as internal standard, were detected by electrospray tandem mass spectrometry. In the first criminal case, alprazolam tested positive in two consecutive 2 cm hair segments at 4.9 and 2.4 pg/mg, from a 12-year-old girl, assaulted by her father who had sedated her three or four times. In the other case, alprazolam was detected in four consecutive 1cm hair segments at 3.1-0.4 pg/mg, obtained from an adolescent who had been forced to prostitute herself. 相似文献
93.
Friedrich Plank Britta Daum Johannes Muntschick Michèle Knodt Christian Hasse Ingrid Ott Arne Niemann 《中东政策》2023,30(3):37-52
The war in Ukraine and the looming threat of climate change are driving the strategic need to diversify sources of energy, including renewables. Therefore, the European Union aims to develop energy relations with non-EU member states, and Morocco has become a key priority. Both Brussels and Rabat are pursuing ambitious green policies and cooperation initiatives, including on hydrogen. Drawing on theories of international institutions and political economy, this article analyzes hydrogen-related relationship patterns and explains demand and supply factors as drivers of institutionalized energy cooperation. We examine the EU's hydrogen approach and development, its relations with Morocco, and the political interests of both sides. Our analysis shows that the complex interdependencies in the evolving EU-Morocco hydrogen relations indicate that demand can be met by supply. However, challenges remain, including the need to deepen the partnership and prepare the market for commercial hydrogen production. 相似文献
94.
Annick Lempérière 《议会、议员及代表》2017,37(2):148-158
SUMMARYBy looking at the various sites where successive Mexican congresses met between 1813 and the 1870s (be they constituent or legislative bodies), this article examines the role of such assemblies in the national political history and public culture of Mexico. The first part of this article follows the steps of successive congresses, identifying periods of serious political conflicts – though congress enjoyed a time of relative flourishing when it occupied a chamber within the National Palace. By analysing the ceremonies, rituals and symbols adopted by congresses, the second part of the articles underlines a historical rupture after 1857, which coincides with the laicization of government, and the secularization of the public space and parliamentary rituals. 相似文献
95.
Valrie Martin Marie‐Hlne Jobin 《Canadian public administration. Administration publique du Canada》2004,47(3):304-331
Au cours des deux derniéres décennies, plusieurs gouvemements ont voulu réformer leur administration publique en mettant en place un cadre de gestion axée sur les résultats. Jusqu à tout récemment, la documentation scientifique n'abor‐dait que trés peu ce théme et, outre les travaux de l'OCDE, le concept de gestion axée sur les résultats et son contenu n' avaient pas été définis. Nous avons donc voulu éla‐borer une définition générique de la gestion axée sur les résultats et comprendre la façon dont elle s'intègre à différents aspects de la gestion des organisations. Pour ce faire, nous avons exploré le cadre de gestion proposé par les réformes mises de l'avant dans huit juridictions. Les pays et provinces canadiennes choisis ont une approche‐type de gestion anglo‐américaine, soit le Canada, le Québec, l'Ontario, l'Alberta, les États‐Unis, l'Australie, le Royaume‐Uni et la Nouvelle‐Zélande. Les modèles étudiés présentent plusieurs similarités. On observe que la gestion de la performance dans ces juridictions se déploie dans des cycles de gestion composés de plusieurs étapes. Les éléments communs de ces cycles sont la planification stratégique, la planification opérationnelle, la réalisation des plans et des programmes, la mesure ds résultats, la reddition de comptes et l'analyse et l'interpréta‐tion des résultats. Ces processus se concrétisent par la publication de documents. Par ailleurs, ces cadres de gestion ont tendance à s'intégrer aux autres enjeux de la gestion des organisations tels que la qualité du service à la clientèle, la gestion des res‐sources humaines et financières et la vérification. Abstract: Over the last two decades, several governments have wanted to reform their public administration by implementing a results‐based management framework. Until very recently, the scientific literature hardly addressed this issue and, other than the OECD work, the concept of results‐based management and its content had not been defined. We therefore wanted to develop a generic definition for results‐based management and to understand how it fits into various aspects of organizational management. To do so, we explored the management framework put forward through reforms introduced in eight jurisdictions. The countries and Canadian provinces chosen, i.e., Canada, Quebec, Ontario, Alberta, United States, Australia, United Kingdom and New‐Zealand, use the typical Anglo‐American management approach. The models considered have several similarities. We found that performance management in those jurisdictions was implemented throughout several steps of management cycles. Common elements in these cycles include strategic planning, operational planning, plan and program delivery, results measurement, accountability, and results analysis and interpretation. The process is documented through published documents. Moreover, these management frameworks tend to become part of the other organizational management components, such as quality of customer service, management of human and financial resources, and auditing. 相似文献
96.
Abstract The way in which, in the special case of self-defense, intent to harm, consequences of the negative act and information regarding the dangerousness of the victim were integrated in a judgment of blame was studied. The sample consisted of men on the street, and also of two sub-populations directly concerned with the special issue of self-defense: police officers and prisoners. Overall, the way in which information was integrated appeared to conform to the proposed model: Blame = f [(Intent + Consequences) × Dangerousness]. The strength of the main effects and of the interactions were, however, extremely variable from one participant to another. A noticeable effect of dangerousness was observed in only onethird of the men on the street and the prisoners, and in only one police officer out of 19. 相似文献
97.
AbstractWe use mixed methods and first-hand household data in Mexico to investigate credit practices by households engaged in agricultural wage employment. Quantitative analysis shows evidence of rationing in the formal sector but also suggests the existence of mitigating mechanisms. Qualitative analysis provides additional insights. First, income patterns associated with agricultural wage generate income smoothing needs that are sometimes better met by the informal sector. Second, family networks can perform key functions as gateways to the formal sector, through specific informal arrangements that inject flexibility into formal rules and procedures. 相似文献
98.
Work and mental health: learning from return-to-work rehabilitation programs designed for workers with musculoskeletal disorders 总被引:1,自引:0,他引:1
Briand C Durand MJ St-Arnaud L Corbière M 《International journal of law and psychiatry》2007,30(4-5):444-457
Despite the high costs associated with mental health problems in the workplace, few studies have yet been published on the design and evaluation of return-to-work rehabilitation programs for workers with mental health problems. In fact, the best-documented return-to-work rehabilitation programs concern workers with musculoskeletal disorders (MSKD). For this clientele, a disability paradigm has been adopted which explains the multicausality of work disability. Long-term work disability is no longer seen simply as the consequence of impairment, but rather as the result of interactions between the worker and three main systems: the health care, work environment and financial compensation systems. A return to work is thus influenced by a complex set of interrelated factors that must be taken into account in any intervention. Parallels can inevitably be drawn in the field of mental health in the workplace, where individual and organizational factors are involved and must be taken into account in the return-to-work process. This paper presents the first results of an exploratory study aimed at determining the possible links between work rehabilitation programs for workers with MSKD and those for workers with mental health problems. To this end, the components of a work rehabilitation program for workers with MSKD, the Therapeutic Return to Work (TRW) program which addresses psychological factors, work environmental factors and factors related to the involvement of the various stakeholders in the rehabilitation process, are described through a multiple-case analysis and mapping of interventions. The results support the relevance of adopting the disability paradigm and considering the return-to-work clinical activities conducted with workers with MSKD (and their mechanisms of action) in the design of work rehabilitation programs for workers with mental health problems. 相似文献
99.
Chèze M Deveaux M Martin C Lhermitte M Pépin G 《Forensic science international》2007,170(2-3):100-104
A rapid and sensitive method using LC-MS/MS triple stage quadrupole for the determination of traces of amphetamine (AP), methamphetamine (MA), 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxymethamphetamine (MDMA, "ecstasy"), 3,4-methylenedioxyethamphetamine (MDEA), and N-methyl-1-(3,4-methylenedioxyphenyl)-2-butanamine (MBDB) in hair, blood and urine has been developed and validated. Chromatography was carried out on an Uptisphere ODB C(18) 5 microm, 2.1 mm x 150 mm column (Interchim, France) with a gradient of acetonitrile and formate 2 mM pH 3.0 buffer. Urine and blood were extracted with Toxitube A (Varian, France). Segmented scalp hair was treated by incubation 15 min at 80 degrees C in NaOH 1M before liquid-liquid extraction with hexane/ethyl acetate (2/1, v/v). The limits of quantification (LOQ) in blood and urine were at 0.1 ng/mL for all analytes. In hair, LOQ was <5 pg/mg for MA, MDMA, MDEA and MBDB, at 14.7 pg/mg for AP and 15.7 pg/mg for MDA. Calibration curves were linear in the range 0.1-50 ng/mL in blood and urine; in the range 5-500 pg/mg for MA, MDMA, MDEA and MBDB, and 20-500 pg/mg for AP and MDA. Inter-day precisions were <13% for all analytes in all matrices. Accuracy was <20% in blood and urine at 1 and 50 ng/mL and <10% in hair at 20 and 250 pg/mg. This method was applied to the determination of MDMA in a forensic case of single administration of ecstasy to a 16-year-old female without her knowledge during a party. She suffered from hyperactivity, sweating and agitation. A first sample of urine was collected a few hours after (T+12h) and tested positive to amphetamines by immunoassay by a clinical laboratory. Blood and urine were sampled for forensic purposes at day 8 (D+8) and scalp hair at day 60 (D+60). No MDMA was detected in blood, but urine and hair were tested positive, respectively at 0.42 ng/mL and at 22 pg/mg in hair only in the segment corresponding to the period of the offence, while no MDA was detectable. This method allows the detection of MDMA up to 8 days in urine after single intake. 相似文献
100.
Campaign Strategies in the 2015 Swiss National Elections: Nationalization,Coordination, and Personalization 下载免费PDF全文
This research note examines parties' campaign strategies in the 2015 Swiss elections. We base our analyses on a collection of more than 5000 party advertisements, which were published in the forefront of the national elections in more than 50 daily and weekly national and cantonal print media. By comparing the amount of party and candidate ads, as well as the content and nature of the political advertisements, we explore the degree of professionalization of electoral campaigns in the most recent federal elections in terms of nationalization, coordination and personalization. First results show that although national campaign coordination exists, Swiss elections are to a considerable extent still cantonal and personal affairs. 相似文献