DNA mixture genotyping by probabilistic computer interpretation of binomially-sampled laser captured cell populations: Combining quantitative data for greater identification information

Two person DNA admixtures are frequently encountered in criminal cases and their interpretation can be challenging, particularly if the amount of DNA contributed by both individuals is approximately equal. Due to an inevitable degree of uncertainty in the constituent genotypes, reduced statistical weight is given to the mixture evidence compared to that expected from the constituent single source contributors. The ultimate goal of mixture analysis, then, is to precisely discern the constituent genotypes and here we posit a novel strategy to accomplish this. We hypothesised that LCM-mediated isolation of multiple groups of cells (‘binomial sampling’) from the admixture would create separate cell sub-populations with differing constituent weight ratios. Furthermore we predicted that interpreting the resulting DNA profiling data by the quantitative computer-based TrueAllele® interpretation system would result in an efficient recovery of the constituent genotypes due to newfound abilities to compute a maximum LR from sub-samples with skewed weight ratios, and to jointly interpret all possible pairings of sub-samples using a joint likelihood function.As a proof of concept, 10 separate cell samplings of size 20 recovered by LCM from each of two 1:1 buccal cell mixtures were DNA-STR profiled using a specifically developed LCN methodology, with the data analyzed by the TrueAllele® Casework system. In accordance with the binomial sampling hypothesis, the sub-samples exhibited weight ratios that were well dispersed from the 50% center value (50 ± 35% at the 95% level). The maximum log(LR) information for a genotype inferred from a single 20 cell sample was 18.5 ban, with an average log(LR) information of 11.7 ban. Co-inferring genotypes using a joint likelihood function with two sub-samples essentially recovered the full genotype information. We demonstrate that a similar gain in genotype information can be obtained with standard (28-cycle) PCR conditions using the same joint interpretation methods. Finally, we discuss the implications of this work for routine forensic practice.     

Participation in Micro-Finance Programmes and Women’s Mental Health in South Asia: A Modified Systematic Review

Micro-finance provides financial services to poor women from developing countries where cultural and social constraints limit their opportunities for economic advancement. Using Forbes’s process of conducting systematic review, 12 quantitative studies from South Asia reporting on the impact of micro-finance on women’s mental health outcomes were analysed. Overall, studies revealed that the duration and depth of involvement in micro-finance activities would make a difference in women’s mental health and not just receiving loans; however, rigorous programme evaluation is needed. Collaborative actions by micro-finance and health care practitioners to recognise women’s mental health needs and to strengthen measures to optimise the beneficial effects of micro-finance must be considered.     

All the world is a stage: global governance,human resources,and the ‘problem’ of smallness

Abstract

The involvement of small island states (SISs) in a growing number of international organisations (IOs) has placed increased pressure on domestic bureaucracies and political systems. Rapid turnover among SIS leaders, combined with generational change and decreased local support, has amplified disadvantages. Growing complexity has therefore further exposed the long-standing vulnerabilities of SISs. They can play a creative role at the margins, and on certain issues in certain IOs, but in general asymmetries prevail. The lesson is that national sovereignty does not always equal control, and what might superficially appear to be equal access is constrained by the availability of technical expertise to the detriment of SISs.     

Incomplete Democratization and the Outbreak of Military Disputes

Whereas most research on the democratic peace has focused on relations within pairs of states, research on the relationship between democratization and armed conflict has centered primarily on the behavior of individual states. Moreover, the existing literature has placed primary emphasis on explaining the effects of democratization on war, rather than military disputes more generally. In this article, we find that certain types of democratic transitions markedly increase the risk of such disputes within dyads, even when economic and political relations between states are taken into account. Particularly prone to violence are dyads in which either state undergoes an incomplete democratic transition; that is, a shift from an autocratic to a partially democratic (or anocratic) regime that stalls prior to the establishment of consolidated democratic institutions.     

The Supreme Court and Police Practices: The Last Two Terms

The 2003–2004 term of the Supreme Court was noteworthy because it decided a relatively large number of cases dealing with police practices, many of which were of special significance. The next two terms (2004–2005 and 2005–2006 terms) were not quite as noteworthy, but still the Court decided eight police practices cases, dealing with such important issues as the detention of the residents of a home while executing a search warrant, the use of canine sniffs during a traffic stop, the validity of anticipatory search warrants, and the validity of third party consent to search when another person with authority to consent is present and objects to the search. These two terms do not provide enough cases to permit a confident prediction about the approach that the two new members of the Court, Chief Justice John Roberts and Associate Justice Samuel Alito, are likely to take in police practices cases. However, their positions in the cases discussed in this article suggest that both will take positions in support of the police in these cases.     

The Nature and Prevalence of Familicide in the United States, 2000–2009

Familicide refers to the killing of multiple family members, most commonly the homicide of an intimate partner and at least one child. This study examines the prevalence of familicide in the United States. Second, it explores the relationship between the prevalence of familicide and the prevalence of financial problems in the United States by making use of Supplementary Homicide Reports data and newspaper reports. In the period of 2000–2009, familicide involving an intimate partner and child(ren) occurred approximately 23 times per year. The majority of the perpetrators were male, who committed the offense with a firearm. Familicides involving an intimate partner and child(ren) with financial motives alone occurred 4 to 5 times per year. The results showed that the association between familicide and financial problems is not a straightforward one. Even though correlational analyses suggest a relationship between the two, the prevalence of familicide motivated by financial problems was unrelated to periods of financial downfall. Directions for future research are discussed.