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Jon Wilson M.S.F.S. Valerie Fuller Ph.D. Gifty Benson M.S.F.S. D.D.S. Denise Juroske M.S.F.S. Eric Duvall M.S.F.S. Jun Fu Ph.D. Jane Pritchard M.T. C.L.S. Robert W. Allen Ph.D. 《Journal of forensic sciences》2010,55(4):1050-1057
Abstract: A method is described for the quantitation of total human and male DNA. Q‐TAT utilizes end‐point, multiplex polymerase chain reaction (PCR) amplification of the amelogenin and SRY loci to quantify DNA and incorporates a cloned nonhuman template to detect PCR inhibition. Standard curves of fluorescence from amelogenin or SRY amplicons were generated from amplification of known amounts of NIST traceable SRM‐female or SRM‐male DNA. Curves showed good linearity up to 500 pg of SRM‐template (R2 > 0.99) and reliably estimated total and male DNA content in casework samples. The nonhuman pRLnull template included in each PCR was a sensitive indicator of known PCR inhibitors including EDTA, hemin, blue denim dye, and humic acid. Finally, the SRY amplicon was a sensitive indicator of male DNA and, in mixtures, could reliably estimate male DNA present in an excess of female DNA. The Q‐TAT multiplex is a reliable quantitation method for forensic DNA typing. 相似文献
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Classroom recording systems (systems that capture audio or video footage of a taught session) are being adopted in universities globally in part encouraged by studies that suggest that use of recording technology is associated with enhanced student engagement and perceptions of a course. Notwithstanding increased adoption as a result of perceived benefits, approximately only 10% of higher education institutions have adopted comprehensive lecture recording systems. This study considered the benefits and advantages of classroom recording systems. Academic concerns over student attendance and use of recordings are discussed with the implications for teachers, cognisant of the synergistic relationship between teachers, students and their learning. 相似文献
236.
Jane A. Flegal Aarti Gupta 《International Environmental Agreements: Politics, Law and Economics》2018,18(1):45-61
This paper examines how notions of equity are being evoked by expert advocates of more research into solar geoengineering. We trace how specific understandings of equity figure centrally—although not always explicitly—in these expert visions. We find that understandings of equity in such “vanguard visions” are narrowly conceived as epistemic challenges, answerable by (more) scientific analysis. Major concerns about equity are treated as empirical matters, requiring scientific assessment of feasibility, risks, or “win–win” distributive outcomes and optimizations, with concurrent calls to delimit risk or reduce scientific uncertainties. We argue that such epistemic framings sidestep, inter alia, the inequality in resources available to diverse non-experts—including the “vulnerable” evoked in expert visions—to project their own equity perspectives onto imagined technological pathways of the future. These may include concerns relating to moral or historical responsibility and/or lack of agency in shaping the directions of innovation. We conclude that the performative power and political implications of specific expert visions of equity, evoked as a rationale to undertake solar geoengineering research, require continued scrutiny. 相似文献
237.
In the post-human rights era the question has arisen on several occasions as to whether the automatic and arbitrary termination
of the registered owner’s title through the common law and statutory principles governing adverse possession of land is contrary
to the Article 1, Protocol 1 of the European Convention. The matter fell to be decided in J.A. Pye (Oxford) Ltd v United Kingdom ([2005] 3 EGLR 1) where the European Court of Human Rights held that the automatic termination of a registered owners title
after 12 years possession was indeed a violation of Article 1, Protocol 1. More recently, the decision of the European Court
has been overturned by the Grand Chamber of the European Court of Human Rights where the Grand Chamber has held that a squatters’
right to another persons land are not disproportionate (J. A. Pye (Oxford) Ltd and Another v United Kingdom, The Times, October 1st 2007). This short article examines the decision of the Grand Chamber.
相似文献
Jane WoodEmail: |
238.
Jane J. Bolitho 《Critical Criminology》2012,20(1):61-78
This paper is concerned with the nature and complexities of restorative justice. It uses Braithwaite’s (Br J Criminol 42:563–577, 2002a) framework of constraining, maximising and emerging restorative standards to understand the interactions that underpin success and failure in practice, i.e., ‘restorativeness’. Using qualitative data from observations of youth justice conferences in New South Wales, Australia, the roles of empowerment (as an example of a constraining standard), restoration of communities (as an example of a maximising standard) and remorse over injustice (as an example of an emergent standard) are examined. Findings confirm that restorative justice is best conceived as a continuum of dynamic process and outcome related values. Non-domination is paramount to achieving restorative justice. However, the presence, absence, and nature of other values such as storytelling, respectful listening, victim and support attendance, and apology are also important. They affect where a restorative event falls on the restorative continuum, and they affect the likelihood of other standards being met. 相似文献
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Budgeting and Financial Management play a crucial role in organizing and disciplining the federal management culture. Consequently, addressing improvements in their practice is essential in the National Performance Review's examination of government-wide management systems. This article briefly describes the recommendations and anticipated implementing actions relating to budgeting and financial management within the context of the National Performance Review's themes of cutting red tape, putting customers first, empowering employees to get results, and cutting back to basics. External factors, such as innovations in private sector management practices, rapid changes in information technology, and statutory reforms such as the Government Performance and Results Act, also influence needed changes in budgetary and financial management practices. 相似文献